FastQCFastQC Report
Thu 26 May 2016
SRR805201_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805201_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23635335
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1237310.5235000900135327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT677300.039.4014471
CAACGCA744600.035.54982813
TCAACGC742950.035.41052612
GTGGTAT757100.035.006346
AACGCAG756600.034.99530814
GGTATCA761150.034.8571248
ACGCAGA759550.034.84082815
GTATCAA764400.034.7119989
AGTGGTA769100.034.652655
TGGTATC767400.034.511997
ATCAACG766000.034.4646211
TATCAAC776300.034.17989310
CGCAGAG781800.033.91839216
AGAGTAC773750.033.88252319
CAGTGGT804400.033.175784
AGCAGTG814050.032.831582
GCAGTGG816400.032.73423
CAGAGTA808400.032.54651318
GCAGAGT832100.031.64493417
ACATGGG549250.031.10514524
Produced by FastQC (version 0.11.5)