##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805201_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23635335
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.80585987040167	37.0	35.0	37.0	35.0	37.0
2	36.09510540045233	37.0	35.0	37.0	35.0	37.0
3	37.94953297679089	39.0	38.0	39.0	35.0	39.0
4	37.938266244163664	39.0	38.0	39.0	35.0	39.0
5	37.93038080484156	39.0	38.0	39.0	35.0	39.0
6	37.90138168128355	39.0	38.0	39.0	35.0	39.0
7	37.865162224271415	39.0	38.0	39.0	35.0	39.0
8	38.809638619465304	40.0	39.0	40.0	36.0	40.0
9	38.693886039694384	40.0	39.0	40.0	36.0	40.0
10	38.683279462719696	40.0	39.0	40.0	36.0	40.0
11	38.673181573267314	40.0	39.0	40.0	36.0	40.0
12	38.433686258307745	40.0	38.0	40.0	36.0	40.0
13	38.65520069844578	40.0	39.0	40.0	36.0	40.0
14	38.683064403360476	40.0	39.0	40.0	36.0	40.0
15	38.61257688964425	40.0	39.0	40.0	36.0	40.0
16	38.58456615910035	40.0	39.0	40.0	36.0	40.0
17	38.1452600946845	40.0	38.0	40.0	34.0	40.0
18	38.262475865055436	40.0	38.0	40.0	34.0	40.0
19	38.34353238488052	40.0	38.0	40.0	35.0	40.0
20	38.275745573312165	40.0	38.0	40.0	35.0	40.0
21	38.27860036678135	40.0	38.0	40.0	35.0	40.0
22	38.17236019713704	40.0	38.0	40.0	35.0	40.0
23	37.89704114623296	40.0	38.0	40.0	34.0	40.0
24	37.84035567932504	40.0	38.0	40.0	34.0	40.0
25	37.76815776040407	40.0	38.0	40.0	34.0	40.0
26	37.547587542127076	39.0	37.0	40.0	34.0	40.0
27	37.509666861078976	39.0	37.0	40.0	33.0	40.0
28	37.43650961579347	39.0	37.0	40.0	33.0	40.0
29	37.132767527940686	39.0	37.0	40.0	33.0	40.0
30	37.089312759899535	38.0	36.0	40.0	33.0	40.0
31	36.986677108659556	38.0	36.0	40.0	33.0	40.0
32	36.798552083141615	38.0	36.0	40.0	32.0	40.0
33	36.68403701491855	38.0	36.0	40.0	32.0	40.0
34	36.57814099102043	38.0	36.0	40.0	31.0	40.0
35	36.32771945055993	38.0	35.0	40.0	31.0	40.0
36	36.19594810058753	38.0	35.0	39.0	31.0	40.0
37	36.03870268815737	38.0	35.0	39.0	31.0	40.0
38	36.00154865585785	38.0	35.0	39.0	31.0	40.0
39	35.87620145853655	38.0	35.0	39.0	31.0	40.0
40	35.78433536059463	38.0	35.0	39.0	30.0	40.0
41	35.54058929141474	37.0	34.0	38.0	30.0	40.0
42	35.334128964112416	37.0	34.0	38.0	30.0	40.0
43	35.04571278553911	37.0	34.0	38.0	29.0	40.0
44	34.88847685044447	36.0	34.0	38.0	29.0	39.0
45	34.79989879559566	36.0	33.0	38.0	29.0	39.0
46	34.7109043303173	36.0	33.0	38.0	29.0	39.0
47	34.52662257590172	36.0	33.0	38.0	28.0	39.0
48	34.36040018049247	36.0	33.0	38.0	28.0	39.0
49	34.05811908314394	36.0	33.0	38.0	28.0	39.0
50	33.81841522449333	35.0	32.0	38.0	27.0	39.0
51	33.53585747779754	35.0	32.0	38.0	27.0	39.0
52	33.051080384517505	34.0	31.0	37.0	26.0	38.0
53	35.27285320897715	37.0	34.0	39.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	5.0
12	29.0
13	75.0
14	397.0
15	1098.0
16	2356.0
17	3878.0
18	5780.0
19	9294.0
20	14873.0
21	23223.0
22	33848.0
23	48131.0
24	65203.0
25	86625.0
26	110910.0
27	142720.0
28	180936.0
29	227140.0
30	286323.0
31	366408.0
32	477543.0
33	643592.0
34	918984.0
35	1408982.0
36	2486424.0
37	5535995.0
38	9766803.0
39	787757.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.838897354321404	37.76997025851336	14.803784249303003	18.587348137862232
2	29.767105056898917	29.47261377932659	25.795187586721326	14.965093577053171
3	24.961478227408243	24.899676691699103	26.003447803891927	24.135397277000727
4	22.495082045589793	30.42880500741792	23.66949738601124	23.40661556098105
5	22.685948813503174	30.572894354998564	22.80176693074162	23.93938990075664
6	24.372195274575123	25.363317253595092	28.03340422295686	22.231083248872928
7	21.77212212139155	26.578738993968145	27.172384059713984	24.47675482492632
8	23.731345462207326	25.34337253946263	27.02215559881	23.90312639952004
9	24.96968204597058	25.924629373774476	26.15537287709271	22.950315703162236
10	22.31334990597764	27.158933858986977	28.518449177894027	22.00926705714135
11	22.319666719342035	29.184198150777213	26.556213398286932	21.93992173159382
12	22.636099721032092	25.865497569634616	28.610167784801867	22.888234924531428
13	22.571793460934657	26.581006784968352	25.919433763050108	24.92776599104688
14	22.532695220947787	28.791946464900963	26.062283441296685	22.613074872854565
15	22.705715827594574	28.096999682890043	25.846280579479835	23.351003910035548
16	23.35369902732498	26.018687697889618	25.461864619223718	25.165748655561682
17	25.364095749013078	26.07148999580501	25.50042552813404	23.06398872704787
18	22.911268234615672	25.988525231396125	25.597018193310987	25.503188340677212
19	23.57011652257097	28.31234251598296	25.28250604444574	22.83503491700033
20	25.402923207984994	26.22330083326511	25.639687357932516	22.73408860081738
21	23.433947519677638	27.95891828907862	25.57488607629213	23.032248114951617
22	25.55871537255554	25.903195364059783	25.70774647365904	22.830342789725638
23	23.225695764413747	26.154535148327707	27.990062336751308	22.62970675050724
24	23.026760568445507	27.914044797757253	25.565095650220314	23.49409898357692
25	23.20215473992647	26.067026340011683	25.399496135764522	25.33132278429732
26	23.25574822611992	27.386838392601586	26.723877617981724	22.633535763296774
27	23.3846865297234	25.42171287185056	28.04951992429978	23.14408067412626
28	25.111321671556592	25.87889699892132	26.11401107705899	22.8957702524631
29	25.245743290712824	25.794248315075713	26.30660407394268	22.653404320268784
30	24.840921442408156	25.52796903449856	26.4291705617881	23.20193896130518
31	24.435790734508313	26.125785820255988	26.184473374293194	23.253950070942512
32	23.87727950545232	26.397810735494126	26.578383593886016	23.146526165167533
33	23.691371414875228	25.90142259460253	26.669700260224786	23.737505730297457
34	23.841396790017995	26.24652876720385	26.454403967618823	23.457670475159333
35	23.658598450159477	26.187367346390477	26.768408402081036	23.38562580136901
36	23.64138270094331	25.913861597476828	26.70204589865132	23.742709802928537
37	23.87905227490958	26.220660718369338	26.41173903395065	23.48854797277043
38	23.711955002964842	26.203411121526308	26.74796866640562	23.336665209103234
39	23.73924465212784	25.880999782740545	26.699460786149213	23.6802947789824
40	23.869972649002015	26.24970621317616	26.345135366179495	23.535185771642332
41	23.658018809549347	26.339030100483026	26.705426430384843	23.29752465958278
42	23.731531624155107	25.943105947091503	26.694760196967803	23.630602231785588
43	24.0254136444438	26.212761528448823	26.283088435175557	23.478736391931825
44	23.75283447431568	26.17620186047712	26.638704295919645	23.432259369287554
45	23.73463714392032	25.813858783892847	26.645291890298996	23.80621218188784
46	23.921222187034793	26.145683993901503	26.33605574027193	23.597038078791773
47	23.729056516440323	26.135161612898656	26.649654003211715	23.48612786744931
48	23.784909331727263	25.77666870387071	26.647805076594004	23.79061688780802
49	23.91302259942582	26.220432246887977	26.30256774443857	23.563977409247638
50	23.7536298935471	26.19721700580931	26.6218651015524	23.42728799909119
51	23.78048798546752	25.851404263997104	26.55414446209457	23.813963288440803
52	24.085061624893406	26.137607103939924	26.1475752300528	23.62975604111387
53	23.900257813142904	26.183195626378897	26.42866284738507	23.48788371309313
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6625.0
1	4466.0
2	2307.0
3	1895.0
4	1483.0
5	1486.5
6	1490.0
7	1670.0
8	2296.5
9	2743.0
10	3883.0
11	5023.0
12	7494.0
13	9965.0
14	13120.0
15	16275.0
16	23013.0
17	29751.0
18	41388.5
19	53026.0
20	70495.5
21	87965.0
22	189408.0
23	290851.0
24	257293.5
25	253629.5
26	283523.0
27	323731.5
28	363940.0
29	406994.0
30	450048.0
31	492902.0
32	535756.0
33	594306.0
34	652856.0
35	729371.0
36	805886.0
37	890939.0
38	975992.0
39	1051934.5
40	1127877.0
41	1216174.5
42	1382946.5
43	1461421.0
44	1530815.0
45	1600209.0
46	1649045.0
47	1697881.0
48	1725702.0
49	1753523.0
50	1746443.5
51	1739364.0
52	1700689.5
53	1662015.0
54	1596343.0
55	1530671.0
56	1432185.5
57	1333700.0
58	1211833.5
59	964671.0
60	839375.0
61	732160.0
62	624945.0
63	532262.5
64	439580.0
65	361410.0
66	283240.0
67	225861.5
68	168483.0
69	129498.0
70	90513.0
71	66929.5
72	43346.0
73	32756.0
74	22166.0
75	16724.5
76	8227.0
77	5171.0
78	3828.5
79	2486.0
80	1961.0
81	1436.0
82	1049.5
83	663.0
84	457.5
85	252.0
86	170.5
87	89.0
88	72.0
89	55.0
90	44.0
91	33.0
92	23.5
93	8.5
94	3.0
95	5.0
96	7.0
97	5.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.3635335E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.108079411117515
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.05748512952671	30.459930380252302
2	15.172145497423692	13.384283968747862
3	5.864667990919878	7.760377243899989
4	2.874394359937626	5.071360587479884
5	1.6678703568407023	3.67832790734893
6	1.0588662138368627	2.8022733027399407
7	0.7560778934980185	2.334440063718073
8	0.5215244994496226	1.8402755229253813
9	0.39312625010246677	1.5606039472303002
>10	2.3921644358036396	20.09919696915992
>50	0.18259000800377417	5.477484794834381
>100	0.05685400064458975	4.044501836533366
>500	0.0017605004885112238	0.5159069348388461
>1k	4.247982608261055E-4	0.2955664130709627
>5k	2.883915779030063E-5	0.08684216137584774
>10k+	1.9226105193063755E-5	0.588627965843857
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	123731	0.5235000900135327	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3115955411674936E-4	0.0	0.0	0.0	4.230953358604817E-6
2	1.3962146083395898E-4	0.0	0.0	0.0	1.2692860075814454E-5
3	1.3962146083395898E-4	0.0	0.0	0.0	1.2692860075814454E-5
4	1.3962146083395898E-4	0.0	0.0	4.230953358604817E-6	2.115476679302409E-5
5	1.4808336755116862E-4	0.0	0.0	1.2692860075814454E-5	2.115476679302409E-5
6	1.5231432090977344E-4	4.230953358604817E-6	0.0	1.692381343441927E-5	2.115476679302409E-5
7	1.5654527426837826E-4	8.461906717209635E-6	0.0	2.115476679302409E-5	2.115476679302409E-5
8	1.6077622762698308E-4	1.2692860075814454E-5	0.0	2.115476679302409E-5	3.384762686883854E-5
9	1.6077622762698308E-4	1.2692860075814454E-5	0.0	2.115476679302409E-5	5.500239366186263E-5
10	1.6077622762698308E-4	1.692381343441927E-5	0.0	2.5385720151628907E-5	6.346430037907227E-5
11	1.650071809855879E-4	2.115476679302409E-5	0.0	2.5385720151628907E-5	6.769525373767708E-5
12	1.6923813434419272E-4	2.115476679302409E-5	0.0	2.5385720151628907E-5	6.769525373767708E-5
13	1.7346908770279751E-4	2.115476679302409E-5	0.0	2.9616673510233725E-5	8.885002053070117E-5
14	1.9462385449582162E-4	2.115476679302409E-5	0.0	2.9616673510233725E-5	8.885002053070117E-5
15	2.0731671457163608E-4	2.115476679302409E-5	0.0	2.9616673510233725E-5	8.885002053070117E-5
16	2.0731671457163608E-4	2.115476679302409E-5	0.0	2.9616673510233725E-5	9.308097388930599E-5
17	2.157786212888457E-4	2.115476679302409E-5	0.0	3.384762686883854E-5	1.0154288060651563E-4
18	2.2424052800605534E-4	2.115476679302409E-5	0.0	5.0771440303257815E-5	1.0154288060651563E-4
19	2.538572015162891E-4	2.5385720151628907E-5	0.0	5.500239366186263E-5	1.2269764739953972E-4
20	2.707810149507083E-4	2.5385720151628907E-5	0.0	5.500239366186263E-5	1.2692860075814454E-4
21	3.046286418195469E-4	2.5385720151628907E-5	0.0	5.923334702046745E-5	1.2692860075814454E-4
22	3.4270722204699026E-4	2.9616673510233725E-5	0.0	5.923334702046745E-5	1.3115955411674936E-4
23	3.7232389555722395E-4	2.9616673510233725E-5	0.0	5.923334702046745E-5	1.3115955411674936E-4
24	4.1463342914327216E-4	2.9616673510233725E-5	0.0	6.346430037907227E-5	1.3115955411674936E-4
25	4.780977295223444E-4	2.9616673510233725E-5	0.0	6.769525373767708E-5	1.3539050747535415E-4
26	5.373310765428119E-4	2.9616673510233725E-5	0.0	6.769525373767708E-5	1.3962146083395898E-4
27	6.177191903563034E-4	3.384762686883854E-5	0.0	6.769525373767708E-5	1.6077622762698308E-4
28	7.150311176042142E-4	3.807858022744336E-5	0.0	7.615716045488672E-5	1.6923813434419272E-4
29	7.996501847763105E-4	3.807858022744336E-5	0.0	7.615716045488672E-5	1.7346908770279751E-4
30	9.05424018741431E-4	3.807858022744336E-5	0.0	7.615716045488672E-5	1.7346908770279751E-4
31	0.0010408145262167851	4.230953358604818E-5	0.0	8.038811381349154E-5	1.7346908770279751E-4
32	0.0011423574068233008	4.230953358604818E-5	0.0	8.461906717209636E-5	1.8193099442000716E-4
33	0.0012735169609400501	4.230953358604818E-5	0.0	8.461906717209636E-5	1.9885480785442644E-4
34	0.0013835217482637753	4.230953358604818E-5	0.0	8.885002053070117E-5	1.9885480785442644E-4
35	0.0014977574889461056	4.230953358604818E-5	0.0	9.308097388930599E-5	2.157786212888457E-4
36	0.0016119932296284356	4.230953358604818E-5	0.0	9.308097388930599E-5	2.2847148136466016E-4
37	0.0017727694572554186	4.230953358604818E-5	0.0	9.308097388930599E-5	2.369333880818698E-4
38	0.0019800861718270546	4.230953358604818E-5	0.0	9.731192724791081E-5	2.369333880818698E-4
39	0.0022381743267019484	4.230953358604818E-5	0.0	9.731192724791081E-5	2.580881548748939E-4
40	0.0024708767614252136	4.230953358604818E-5	0.0	1.0154288060651563E-4	2.7501196830931313E-4
41	0.0026824244293554545	4.230953358604818E-5	0.0	1.0577383396512045E-4	2.8347387502652277E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	67730	0.0	39.401447	1
CAACGCA	74460	0.0	35.549828	13
TCAACGC	74295	0.0	35.410526	12
GTGGTAT	75710	0.0	35.00634	6
AACGCAG	75660	0.0	34.995308	14
GGTATCA	76115	0.0	34.857124	8
ACGCAGA	75955	0.0	34.840828	15
GTATCAA	76440	0.0	34.711998	9
AGTGGTA	76910	0.0	34.65265	5
TGGTATC	76740	0.0	34.51199	7
ATCAACG	76600	0.0	34.46462	11
TATCAAC	77630	0.0	34.179893	10
CGCAGAG	78180	0.0	33.918392	16
AGAGTAC	77375	0.0	33.882523	19
CAGTGGT	80440	0.0	33.17578	4
AGCAGTG	81405	0.0	32.83158	2
GCAGTGG	81640	0.0	32.7342	3
CAGAGTA	80840	0.0	32.546513	18
GCAGAGT	83210	0.0	31.644934	17
ACATGGG	54925	0.0	31.105145	24
>>END_MODULE