FastQCFastQC Report
Thu 26 May 2016
SRR805200_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805200_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19132529
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1228110.6418963222269256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT653250.041.1973951
TCAACGC744200.036.0110212
CAACGCA744100.035.90847813
AGTGGTA752700.035.6387025
ACGCAGA750400.035.59448215
GTGGTAT755000.035.5176856
AACGCAG753500.035.4511614
GGTATCA757800.035.3275268
TGGTATC761100.035.2330177
ATCAACG762900.035.1591311
GTATCAA761000.035.1542749
CGCAGAG767500.034.819816
TATCAAC770150.034.74881710
AGCAGTG784200.034.318032
AGAGTAC757400.034.30056819
CAGTGGT789250.034.0478324
GCAGTGG790900.034.045143
CAGAGTA785550.033.28680818
GAGTACT270250.032.9391320
GCAGAGT813450.032.68246517
Produced by FastQC (version 0.11.5)