##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805200_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19132529
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.213900316053355	37.0	36.0	37.0	35.0	37.0
2	36.230843711252184	37.0	37.0	37.0	35.0	37.0
3	38.048162582165695	39.0	39.0	39.0	35.0	39.0
4	38.06196054896872	39.0	39.0	39.0	35.0	39.0
5	38.061755244170804	39.0	39.0	39.0	35.0	39.0
6	38.052347862637504	39.0	39.0	39.0	35.0	39.0
7	38.02759551547001	39.0	39.0	39.0	35.0	39.0
8	38.984329515455066	40.0	40.0	40.0	37.0	40.0
9	38.95214866785253	40.0	40.0	40.0	37.0	40.0
10	38.94315210498309	40.0	39.0	40.0	37.0	40.0
11	38.95002066898736	40.0	40.0	40.0	37.0	40.0
12	38.93799329926535	40.0	40.0	40.0	37.0	40.0
13	39.00741982411212	40.0	40.0	40.0	37.0	40.0
14	38.94436344510441	40.0	40.0	40.0	37.0	40.0
15	38.94880305682537	40.0	40.0	40.0	37.0	40.0
16	38.948655193466585	40.0	40.0	40.0	37.0	40.0
17	38.92144174980736	40.0	40.0	40.0	37.0	40.0
18	38.849153658672094	40.0	40.0	40.0	37.0	40.0
19	38.753828466691466	40.0	39.0	40.0	36.0	40.0
20	38.75745961237011	40.0	39.0	40.0	36.0	40.0
21	38.69280846248815	40.0	39.0	40.0	36.0	40.0
22	38.716706035046386	40.0	39.0	40.0	36.0	40.0
23	38.65828113993712	40.0	39.0	40.0	36.0	40.0
24	38.27356644801113	40.0	39.0	40.0	35.0	40.0
25	38.39239726227515	40.0	39.0	40.0	35.0	40.0
26	38.49786847311194	40.0	39.0	40.0	35.0	40.0
27	38.3917729328935	40.0	39.0	40.0	35.0	40.0
28	38.51632912721575	40.0	39.0	40.0	36.0	40.0
29	38.559640651792556	40.0	39.0	40.0	36.0	40.0
30	38.55128519601355	40.0	39.0	40.0	36.0	40.0
31	38.42111766823926	40.0	39.0	40.0	35.0	40.0
32	38.29675752745494	40.0	39.0	40.0	35.0	40.0
33	38.370529269810596	40.0	39.0	40.0	35.0	40.0
34	38.35107272018247	40.0	39.0	40.0	35.0	40.0
35	38.31050207737827	40.0	39.0	40.0	35.0	40.0
36	38.2681295295567	40.0	39.0	40.0	35.0	40.0
37	38.27177395105477	40.0	38.0	40.0	35.0	40.0
38	38.24217859541726	40.0	38.0	40.0	35.0	40.0
39	38.201038555854275	40.0	38.0	40.0	35.0	40.0
40	38.15562934727552	40.0	38.0	40.0	35.0	40.0
41	38.08714963923483	40.0	38.0	40.0	35.0	40.0
42	37.99380494862964	40.0	38.0	40.0	34.0	40.0
43	37.73873600296124	40.0	38.0	40.0	33.0	40.0
44	37.70898630285625	40.0	38.0	40.0	33.0	40.0
45	37.856877519955674	40.0	38.0	40.0	34.0	40.0
46	37.88906855962429	40.0	38.0	40.0	34.0	40.0
47	37.866170436746756	40.0	38.0	40.0	34.0	40.0
48	37.84273171623051	40.0	38.0	40.0	34.0	40.0
49	37.691784604115846	40.0	37.0	40.0	34.0	40.0
50	37.680614517819365	40.0	37.0	40.0	34.0	40.0
51	37.298657171772746	40.0	37.0	40.0	33.0	40.0
52	37.28383477166035	40.0	37.0	40.0	33.0	40.0
53	37.07988598893539	39.0	36.0	40.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	5.0
12	12.0
13	44.0
14	123.0
15	434.0
16	1008.0
17	2053.0
18	3448.0
19	5683.0
20	9032.0
21	13534.0
22	19354.0
23	26424.0
24	35160.0
25	45354.0
26	58177.0
27	73324.0
28	93160.0
29	115780.0
30	144191.0
31	180132.0
32	226058.0
33	287347.0
34	377419.0
35	530605.0
36	803221.0
37	1407226.0
38	3153756.0
39	1.1520465E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.698889153650306	38.493473601947755	14.860278011338698	18.947359233063228
2	29.693586247798187	29.71943750875799	25.852588541744797	14.734387701699028
3	25.154468601615605	24.76285283560788	26.032256373425593	24.050422189350922
4	22.063314264413243	30.351183578501306	23.775644087616435	23.809858069469016
5	22.62531001521022	30.984262456886906	22.863746867965023	23.526680659937846
6	24.762424246162126	25.223931452031252	28.086213798499926	21.9274305033067
7	21.711755931481928	26.463108980522126	27.687965349484116	24.13716973851183
8	24.10588793567228	25.213566904824763	27.016177526766068	23.664367632736898
9	25.148128613838765	25.796239483029137	26.37258252685779	22.683049376274305
10	22.02891734804113	27.112578791857572	29.08128873083114	21.777215129270154
11	22.01941520642671	29.576199779966363	26.673786826613462	21.730598186993472
12	22.053623961578733	25.79516800941475	29.306543844778698	22.844664184227813
13	22.323023788439052	26.549417486836163	25.872857686508667	25.25470103821612
14	22.391551059454816	29.30010977639182	25.979356937078208	22.328982227075155
15	22.44967196965963	28.57578315966488	25.77326290737623	23.20128196329926
16	23.234267670520715	25.922835397244135	25.369683223791274	25.473213708443875
17	25.672350999703177	25.826721600683317	25.58689444558009	22.914032954033416
18	22.653215369489313	25.97258313315506	25.554107352979837	25.820094144375787
19	23.55102663113695	28.74893329574987	25.147098953828845	22.552941119284334
20	25.702195459889282	26.227322064950222	25.56708002376476	22.503402451395736
21	23.355987073115113	28.425447571515505	25.466554891933	22.75201046343638
22	25.845840871324434	25.784897542818307	25.76760239067193	22.60165919518533
23	22.74819497202905	26.17618402669088	28.554714329715637	22.520906671564433
24	22.841444536684094	28.385269924326263	25.35846672439383	23.41481881459581
25	23.115276605617584	25.996406434298365	25.314912628644127	25.573404331439924
26	22.85363842908588	27.635504955983603	27.018425008005998	22.49243160692452
27	23.402948977628625	25.30280236345127	28.47028220890192	22.823966450018187
28	25.280379818057508	25.778662089052627	26.28338365513519	22.657574437754676
29	25.1473655155573	25.74387317013867	26.540192360351316	22.568568953952717
30	25.006715003541874	25.424793554474682	26.568406089963332	23.00008535202011
31	24.581888782188702	26.061484082945856	26.329164325322594	23.027462809542847
32	23.758787978316928	26.457657531840145	26.75445049632487	23.029103993518056
33	23.618748990266784	25.945242262536226	26.865016119928526	23.57099262726846
34	23.70654187953929	26.23400701496389	26.695242432403997	23.364208673092826
35	23.43777971014705	26.238768539172213	26.998563545885652	23.32488820479509
36	23.550524867883382	25.929802589087934	26.928452584600816	23.59121995842787
37	23.78850699768964	26.269924901198372	26.64716724067163	23.294400860440355
38	23.63693268150802	26.221191145195704	26.97410781397483	23.167768359321446
39	23.683293515457365	25.9031464162422	26.895132368543646	23.518427699756785
40	23.745146289860582	26.280315581907647	26.564904200589478	23.40963392764229
41	23.539627197219982	26.36996394987824	26.929874247152586	23.16053460574919
42	23.659757682844752	25.9947966105265	26.884428085800888	23.46101762082786
43	24.14930091050692	26.210063499707747	26.39868989614494	23.241945693640396
44	23.684103654043852	26.200801786318994	26.841145778480204	23.27394878115695
45	23.65442122157505	25.810456108546866	26.88185654912636	23.65326612075173
46	23.819454291693482	26.13926261394926	26.563550485144958	23.4777326092123
47	23.588225059008142	26.14932923922394	26.883387972389848	23.379057729378065
48	23.649414042440494	25.806279974801033	26.86882115793474	23.675484824823734
49	23.91364988914952	26.271080002021684	26.4423485259058	23.37292158292299
50	23.62743576659416	26.249451915112736	26.832270840932736	23.290841477360363
51	23.97368377175856	25.851119838888	26.636076182087585	23.539120207265857
52	23.845822996008526	26.183914316816143	26.413403058215668	23.556859628959664
53	23.674554472124413	26.25060701593605	26.617678196123475	23.457160315816065
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5735.0
1	3802.5
2	1870.0
3	1557.0
4	1244.0
5	1329.5
6	1415.0
7	1597.5
8	2101.5
9	2423.0
10	3539.0
11	4655.0
12	6759.5
13	8864.0
14	11531.0
15	14198.0
16	20173.5
17	26149.0
18	36456.0
19	46763.0
20	62602.0
21	78441.0
22	176740.0
23	275039.0
24	236916.5
25	222691.5
26	246589.0
27	278574.5
28	310560.0
29	345267.5
30	379975.0
31	411527.0
32	443079.0
33	489030.0
34	534981.0
35	594461.0
36	653941.0
37	720191.0
38	786441.0
39	848457.5
40	910474.0
41	980190.5
42	1111290.0
43	1172673.0
44	1230013.0
45	1287353.0
46	1328135.0
47	1368917.0
48	1388371.5
49	1407826.0
50	1404006.0
51	1400186.0
52	1367256.5
53	1334327.0
54	1280652.5
55	1226978.0
56	1149025.5
57	1071073.0
58	971602.0
59	769757.5
60	667384.0
61	583040.0
62	498696.0
63	425544.0
64	352392.0
65	289093.0
66	225794.0
67	178677.0
68	131560.0
69	100539.0
70	69518.0
71	50888.5
72	32259.0
73	23822.5
74	15386.0
75	11512.5
76	5552.5
77	3466.0
78	2570.5
79	1675.0
80	1360.0
81	1045.0
82	773.5
83	502.0
84	354.0
85	206.0
86	145.5
87	85.0
88	73.5
89	62.0
90	46.0
91	30.0
92	26.5
93	19.5
94	16.0
95	11.5
96	7.0
97	4.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.9132529E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.614448742039926
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.64508927713813	31.768223549668118
2	15.340769608785635	13.995214979667924
3	5.66067132126106	7.746252054875942
4	2.8171748216382886	5.140155059959407
5	1.5369438186244437	3.5053422517019905
6	1.0226734799022625	2.7989212217307213
7	0.6595474601040393	2.1059425668300826
8	0.4922341317746301	1.7962390858333115
9	0.3647543164609478	1.4974260364481145
>10	2.2491497586258022	19.53958584676398
>50	0.16184345077821088	4.982976240559696
>100	0.047097887832201854	3.497098910813026
>500	0.0016019045636404404	0.4880187780731847
>1k	3.9128712688195854E-4	0.3053354937681731
>5k	2.2990153499997894E-5	0.05543339281007968
>10k+	3.448523024998421E-5	0.7778345304964404
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	122811	0.6418963222269256	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5680101673960615E-4	5.226700557986872E-6	0.0	0.0	1.0453401115973744E-5
2	1.672544178555799E-4	5.226700557986872E-6	0.0	0.0	1.0453401115973744E-5
3	1.672544178555799E-4	1.0453401115973744E-5	0.0	0.0	1.5680101673960615E-5
4	1.7248111841356676E-4	1.0453401115973744E-5	0.0	0.0	1.5680101673960615E-5
5	1.986146212035011E-4	1.0453401115973744E-5	0.0	0.0	2.0906802231947487E-5
6	2.03841321761488E-4	1.0453401115973744E-5	0.0	0.0	2.0906802231947487E-5
7	2.03841321761488E-4	1.0453401115973744E-5	0.0	0.0	2.0906802231947487E-5
8	2.03841321761488E-4	1.0453401115973744E-5	0.0	5.226700557986872E-6	3.136020334792123E-5
9	2.195214234354486E-4	1.0453401115973744E-5	0.0	1.0453401115973744E-5	5.7493706137855586E-5
10	2.2474812399343548E-4	1.5680101673960615E-5	0.0	1.5680101673960615E-5	9.930731060175055E-5
11	2.2474812399343548E-4	1.5680101673960615E-5	0.0	1.5680101673960615E-5	1.2544081339168492E-4
12	2.3520152510940923E-4	1.5680101673960615E-5	0.0	1.5680101673960615E-5	1.3066751394967178E-4
13	2.3520152510940923E-4	1.5680101673960615E-5	0.0	3.136020334792123E-5	1.62027717297593E-4
14	2.561083273413567E-4	2.0906802231947487E-5	0.0	3.136020334792123E-5	1.8816122008752736E-4
15	2.8224183013129104E-4	2.0906802231947487E-5	0.0	3.65869039059081E-5	1.8816122008752736E-4
16	3.0314863236323853E-4	2.6133502789934357E-5	0.0	4.1813604463894975E-5	2.1429472287746174E-4
17	3.1882873403719914E-4	2.6133502789934357E-5	0.0	4.1813604463894975E-5	2.2997482455142234E-4
18	3.345088357111598E-4	2.6133502789934357E-5	0.0	4.1813604463894975E-5	2.3520152510940923E-4
19	3.449622368271335E-4	3.136020334792123E-5	0.0	5.226700557986871E-5	2.5088162678336983E-4
20	3.606423385010941E-4	3.136020334792123E-5	0.0	5.7493706137855586E-5	2.6133502789934355E-4
21	4.233627451969366E-4	3.136020334792123E-5	0.0	5.7493706137855586E-5	2.6133502789934355E-4
22	4.7040305021881846E-4	3.136020334792123E-5	0.0	5.7493706137855586E-5	2.770151295733042E-4
23	5.488035585886215E-4	3.136020334792123E-5	0.0	5.7493706137855586E-5	2.8746853068927793E-4
24	6.11523965284464E-4	3.136020334792123E-5	0.0	5.7493706137855586E-5	3.0314863236323853E-4
25	6.690176714223196E-4	3.136020334792123E-5	0.0	5.7493706137855586E-5	3.0314863236323853E-4
26	7.369647786761488E-4	3.65869039059081E-5	0.0	6.272040669584246E-5	3.1882873403719914E-4
27	8.205919876039388E-4	4.1813604463894975E-5	0.0	6.272040669584246E-5	3.292821351531729E-4
28	8.728589931838076E-4	4.1813604463894975E-5	0.0	6.794710725382933E-5	3.345088357111598E-4
29	9.930731060175057E-4	4.1813604463894975E-5	0.0	6.794710725382933E-5	3.345088357111598E-4
30	0.0011080605182932168	4.1813604463894975E-5	0.0	7.840050836980308E-5	3.449622368271335E-4
31	0.0012387280322428886	4.1813604463894975E-5	0.0	7.840050836980308E-5	3.449622368271335E-4
32	0.0014321159528884028	4.1813604463894975E-5	0.0	7.840050836980308E-5	3.5541563794310723E-4
33	0.0016725441785557988	4.1813604463894975E-5	0.0	8.362720892778995E-5	3.763224401750547E-4
34	0.0018345718958533918	4.1813604463894975E-5	0.0	8.885390948577681E-5	3.815491407330416E-4
35	0.0020122797148249454	4.1813604463894975E-5	0.0	9.930731060175055E-5	3.972292424070022E-4
36	0.0022527079404923416	4.1813604463894975E-5	0.0	9.930731060175055E-5	4.1290934408096287E-4
37	0.002446095861137856	4.1813604463894975E-5	0.0	9.930731060175055E-5	4.181360446389497E-4
38	0.0026290303806673966	4.1813604463894975E-5	0.0	1.1498741227571117E-4	4.338161463129103E-4
39	0.0028276450018708973	4.1813604463894975E-5	0.0	1.2544081339168492E-4	4.7040305021881846E-4
40	0.0030367130241903723	4.1813604463894975E-5	0.0	1.3589421450765866E-4	4.7040305021881846E-4
41	0.003287594650973742	4.704030502188184E-5	0.0	1.3589421450765866E-4	4.756297507768053E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	65325	0.0	41.197395	1
TCAACGC	74420	0.0	36.01102	12
CAACGCA	74410	0.0	35.908478	13
AGTGGTA	75270	0.0	35.638702	5
ACGCAGA	75040	0.0	35.594482	15
GTGGTAT	75500	0.0	35.517685	6
AACGCAG	75350	0.0	35.45116	14
GGTATCA	75780	0.0	35.327526	8
TGGTATC	76110	0.0	35.233017	7
ATCAACG	76290	0.0	35.15913	11
GTATCAA	76100	0.0	35.154274	9
CGCAGAG	76750	0.0	34.8198	16
TATCAAC	77015	0.0	34.748817	10
AGCAGTG	78420	0.0	34.31803	2
AGAGTAC	75740	0.0	34.300568	19
CAGTGGT	78925	0.0	34.047832	4
GCAGTGG	79090	0.0	34.04514	3
CAGAGTA	78555	0.0	33.286808	18
GAGTACT	27025	0.0	32.93913	20
GCAGAGT	81345	0.0	32.682465	17
>>END_MODULE