FastQCFastQC Report
Thu 26 May 2016
SRR805199_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805199_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28323363
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10886293.8435725305642556No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC954750.33708920794469216No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG786470.27767535938440646No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG709960.2506623242444762No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA462350.16323979606517772No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG406970.14368703321000403No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC312560.11035412708582663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT5696100.045.9685941
AGCAGTG5795200.045.1707532
GTGGTAT5793900.045.0802966
AGTGGTA5795500.045.069885
TCAACGC5811750.044.98470312
GTATCAA5814000.044.982259
CAACGCA5815300.044.95764513
GGTATCA5816600.044.943158
TGGTATC5810400.044.9352957
ATCAACG5826400.044.90829511
ACGCAGA5820900.044.89259315
AACGCAG5825150.044.89090714
TATCAAC5824000.044.88806510
GCAGTGG5850700.044.7185633
CGCAGAG5843200.044.69954716
AGAGTAC5771500.044.66852619
CAGTGGT5859650.044.5760654
CAGAGTA5817300.044.45518
GCAGAGT5873500.044.273317
GAGTACT1686750.044.20521520
Produced by FastQC (version 0.11.5)