##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805199_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28323363
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.602926919377474	37.0	35.0	37.0	32.0	37.0
2	36.007245573204	37.0	35.0	37.0	35.0	37.0
3	38.000904059309626	39.0	38.0	39.0	35.0	39.0
4	37.99359602883316	39.0	39.0	39.0	35.0	39.0
5	37.93870597216863	39.0	38.0	39.0	35.0	39.0
6	37.96494191032329	39.0	38.0	39.0	35.0	39.0
7	37.85404205002069	39.0	38.0	39.0	35.0	39.0
8	38.874042287986775	40.0	39.0	40.0	36.0	40.0
9	38.9206299760378	40.0	40.0	40.0	36.0	40.0
10	38.63271699056359	40.0	39.0	40.0	36.0	40.0
11	38.80960290626505	40.0	39.0	40.0	36.0	40.0
12	38.88338641142297	40.0	39.0	40.0	36.0	40.0
13	38.98489829756445	40.0	40.0	40.0	37.0	40.0
14	39.00073864816124	40.0	40.0	40.0	37.0	40.0
15	38.9763749806123	40.0	40.0	40.0	37.0	40.0
16	38.92134405084594	40.0	40.0	40.0	37.0	40.0
17	38.93361452169363	40.0	40.0	40.0	37.0	40.0
18	38.87843202094328	40.0	40.0	40.0	37.0	40.0
19	38.85997478477397	40.0	40.0	40.0	37.0	40.0
20	38.810784969284896	40.0	40.0	40.0	36.0	40.0
21	38.736223343252	40.0	39.0	40.0	36.0	40.0
22	38.721440458889006	40.0	39.0	40.0	36.0	40.0
23	38.29792666216932	40.0	38.0	40.0	36.0	40.0
24	38.455583611310566	40.0	39.0	40.0	36.0	40.0
25	38.5152026967984	40.0	39.0	40.0	36.0	40.0
26	38.52135097798944	40.0	39.0	40.0	35.0	40.0
27	38.547131214608946	40.0	39.0	40.0	35.0	40.0
28	38.48383583545499	40.0	39.0	40.0	35.0	40.0
29	38.427530445448866	40.0	39.0	40.0	35.0	40.0
30	38.400436064036604	40.0	39.0	40.0	35.0	40.0
31	38.309599852249185	40.0	39.0	40.0	35.0	40.0
32	38.26819336390244	40.0	39.0	40.0	35.0	40.0
33	38.13723702231264	40.0	38.0	40.0	35.0	40.0
34	38.156267071816295	40.0	38.0	40.0	35.0	40.0
35	38.12485028702277	40.0	38.0	40.0	35.0	40.0
36	38.003897877522526	40.0	38.0	40.0	34.0	40.0
37	37.88101359997398	40.0	37.0	40.0	34.0	40.0
38	37.72484683404298	40.0	37.0	40.0	33.0	40.0
39	37.6398154767144	40.0	37.0	40.0	33.0	40.0
40	37.535983633017025	40.0	37.0	40.0	33.0	40.0
41	37.47097641618335	40.0	37.0	40.0	33.0	40.0
42	37.410290684760845	40.0	37.0	40.0	33.0	40.0
43	37.33760708429998	40.0	36.0	40.0	33.0	40.0
44	37.247704730543475	40.0	36.0	40.0	33.0	40.0
45	37.194500808396235	40.0	36.0	40.0	33.0	40.0
46	37.12647767145448	40.0	36.0	40.0	33.0	40.0
47	37.14696160904339	40.0	36.0	40.0	33.0	40.0
48	37.16056391326129	40.0	36.0	40.0	33.0	40.0
49	37.11103910930351	40.0	35.0	40.0	33.0	40.0
50	36.94549407851038	40.0	35.0	40.0	33.0	40.0
51	36.71940528389937	39.0	35.0	40.0	32.0	40.0
52	36.46788176248703	39.0	35.0	40.0	31.0	40.0
53	36.03978934281215	39.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	17.0
12	33.0
13	100.0
14	263.0
15	763.0
16	1678.0
17	3214.0
18	5603.0
19	8981.0
20	13974.0
21	20735.0
22	29866.0
23	42337.0
24	57836.0
25	78699.0
26	103139.0
27	131193.0
28	166010.0
29	203439.0
30	248817.0
31	305922.0
32	382026.0
33	488753.0
34	662981.0
35	964649.0
36	1504078.0
37	2825664.0
38	4841837.0
39	1.5230751E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.304101635105972	41.52701428852216	27.588884836874772	17.57999923949709
2	22.893344268475463	46.87450074343219	12.015935395807341	18.216219592285
3	42.431063006183265	22.900599762817713	15.922166446124352	18.746170784874664
4	21.045495197727757	20.011536059471467	17.6807323339393	41.262236408861476
5	19.42433177868038	40.282956511908566	20.415266365085248	19.877445344325814
6	39.96347467636524	20.850846702067123	18.848923413508487	20.336755208059156
7	20.54788832809155	20.082103244589987	39.83055613840772	19.53945228891075
8	39.40850879890216	21.957936280377442	19.83514810723571	18.798406813484682
9	39.18579160250144	21.52176985480149	20.129392826692225	19.163045716004838
10	19.111914782153516	21.530815390813586	40.19270946038434	19.16456036664855
11	19.139418578224625	40.96621930100603	20.58337140261204	19.310990718157303
12	18.95420752119019	21.381624067735178	40.285353826097555	19.378814584977075
13	18.87954830787573	21.475246424656564	20.86834815484305	38.776857112624654
14	19.300472899351675	41.0581398826121	20.53477900911696	19.10660820891926
15	19.207761451209024	40.82551920123328	20.572447558575583	19.39427178898212
16	19.18469568744361	21.052549444781683	20.904445563191064	38.858309304583635
17	38.92823037998701	21.056726208677972	20.75209077396635	19.262952637368663
18	19.21336813004868	21.1700390239676	20.81335468531756	38.80323816066616
19	19.29819915805902	40.78321137218063	20.845469515749244	19.073119954011112
20	39.12192206836455	21.06115717967531	20.807045406295856	19.009875345664284
21	19.29635262592228	40.64437192716133	20.856778201091444	19.20249724582494
22	38.79864124892231	20.93713942090846	21.098801014554663	19.16541831561457
23	19.31489562168165	21.115938809949935	40.295041941170616	19.2741236271978
24	19.289619668398842	40.45492055445534	20.76247795856728	19.492981818578535
25	19.23250427571048	21.086306029407595	21.03312731613121	38.648062378750716
26	19.60737501404759	34.73546202829092	26.450160597101412	19.20700236056008
27	19.76210946419039	20.970793616563117	40.06745950330828	19.199637415938213
28	33.43838441783908	20.896854656701606	26.519954568954258	19.14480635650505
29	33.628556043998024	20.987398283177036	26.211375393522303	19.172670279302636
30	33.2433899180687	21.03026042493612	26.30751157622066	19.418838080774517
31	27.97899740931188	23.115009329930206	27.71871052176961	21.187282738988305
32	24.529198739570578	25.30689946670528	28.55085040572336	21.61305138800078
33	23.982360428032504	24.22959095641291	29.25805103016898	22.529997585385605
34	23.186024908129728	23.742858501654624	30.10901636221659	22.962100227999056
35	22.814723661169754	24.072558050398182	30.085247998269132	23.02747029016293
36	22.699447802155415	24.332417728784534	30.25395324700672	22.714181222053327
37	23.660071722415164	23.84509212412382	30.094657191661877	22.400178961799135
38	23.968597938034407	23.99000782498886	29.759894684822562	22.281499552154173
39	23.8386733948225	24.07834832325526	29.74982525909794	22.333153022824305
40	23.237727807958397	24.436487291427927	29.474511907360718	22.851272993252955
41	23.50202904930463	25.091836728569273	29.083410751752893	22.322723470373205
42	23.664520346683407	24.955211003721555	29.33031645994863	22.049952189646408
43	23.588035785157295	24.331210245054585	30.068890477447894	22.01186349234023
44	23.589624579538807	24.165647278538216	29.544408268184817	22.700319873738156
45	23.655478341325498	23.77785434589812	29.435702956601588	23.130964356174793
46	23.45460177168933	23.812776046403812	29.34180167799989	23.39082050390697
47	23.43157837577409	23.99947703950269	29.276399839948386	23.292544744774833
48	23.332349340013046	24.06797526127106	29.477283470892917	23.122391927822978
49	22.74286425662094	24.814715681891304	29.259014898760434	23.183405162727322
50	23.67678230865452	24.577716989327854	28.907502968485772	22.837997733531857
51	23.441255898884606	24.35925423121541	29.06250927900052	23.136980590899466
52	23.74655862723646	24.182749767391677	28.929121164036914	23.14157044133495
53	24.383202658526105	24.39939070794665	28.076870673867365	23.140535959659875
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6128.0
1	3570.0
2	1012.0
3	890.5
4	769.0
5	808.0
6	847.0
7	1002.5
8	1422.5
9	1687.0
10	2769.0
11	3851.0
12	5900.0
13	7949.0
14	9923.0
15	11897.0
16	17390.5
17	22884.0
18	32199.5
19	41515.0
20	62618.5
21	83722.0
22	707587.5
23	1331453.0
24	840852.5
25	337635.0
26	325018.0
27	341428.5
28	357839.0
29	384396.5
30	410954.0
31	441919.0
32	472884.0
33	528218.0
34	583552.0
35	659115.0
36	734678.0
37	820542.5
38	906407.0
39	997144.5
40	1087882.0
41	1192801.0
42	1405440.0
43	1513160.0
44	1638899.5
45	1764639.0
46	1851343.0
47	1938047.0
48	2058542.0
49	2179037.0
50	2168571.5
51	2158106.0
52	2132620.0
53	2107134.0
54	2064486.5
55	2021839.0
56	1973079.5
57	1924320.0
58	1688234.0
59	1262050.0
60	1071952.0
61	927769.0
62	783586.0
63	668615.5
64	553645.0
65	452340.0
66	351035.0
67	282544.0
68	214053.0
69	168259.5
70	122466.0
71	91627.0
72	60788.0
73	46200.0
74	31612.0
75	24431.5
76	12724.5
77	8198.0
78	6034.0
79	3870.0
80	3059.5
81	2249.0
82	1712.5
83	1176.0
84	823.0
85	470.0
86	334.5
87	199.0
88	162.5
89	126.0
90	112.0
91	98.0
92	81.0
93	40.5
94	17.0
95	13.5
96	10.0
97	9.0
98	8.0
99	5.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.8323363E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.62282759942263
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.2765589380594	26.063800336078497
2	15.553193671926937	11.703102482786763
3	5.515249044629518	6.2249779192193015
4	2.5511951939736868	3.839327078213904
5	1.5021706120283356	2.8257952980630625
6	0.9366530350697818	2.1143721395343813
7	0.6623268589151535	1.7443026459202358
8	0.4770358318106508	1.4357949487167425
9	0.3804448691080564	1.2882070549383582
>10	2.8320278180975555	22.798968573389292
>50	0.22938766707465913	5.811270924087375
>100	0.07634309304250748	4.94515599201433
>500	0.004965262746801551	1.2802737285081263
>1k	0.0021379802024971734	1.5936013950174694
>5k	1.7006762399259537E-4	0.45445753780824477
>10k+	1.4005569034609424E-4	5.876591945703743
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1088629	3.8435725305642556	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	95475	0.33708920794469216	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	78647	0.27767535938440646	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	70996	0.2506623242444762	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA	46235	0.16323979606517772	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	40697	0.14368703321000403	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC	31256	0.11035412708582663	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.3188149302750523E-4	0.0	0.0	0.0	7.061308362287346E-6
2	3.77779997382373E-4	0.0	0.0	7.061308362287346E-6	1.7653270905718365E-5
3	3.9543326828809133E-4	3.530654181143673E-6	0.0	7.061308362287346E-6	2.8245233449149383E-5
4	4.201478475560971E-4	7.061308362287346E-6	0.0	1.0591962543431018E-5	3.88371959925804E-5
5	4.3427046428067174E-4	7.061308362287346E-6	0.0	1.0591962543431018E-5	4.942915853601142E-5
6	4.660463519109648E-4	7.061308362287346E-6	3.530654181143673E-6	1.0591962543431018E-5	5.2959812717155095E-5
7	4.660463519109648E-4	1.0591962543431018E-5	3.530654181143673E-6	1.4122616724574692E-5	6.002112107944244E-5
8	4.695770060921085E-4	1.0591962543431018E-5	3.530654181143673E-6	1.7653270905718365E-5	6.002112107944244E-5
9	4.801689686355395E-4	1.0591962543431018E-5	3.530654181143673E-6	1.7653270905718365E-5	7.061308362287346E-5
10	4.907609311789705E-4	1.0591962543431018E-5	3.530654181143673E-6	2.471457926800571E-5	7.76743919851608E-5
11	4.907609311789705E-4	1.0591962543431018E-5	3.530654181143673E-6	2.471457926800571E-5	9.179700870973549E-5
12	5.013528937224016E-4	1.4122616724574692E-5	3.530654181143673E-6	2.8245233449149383E-5	9.532766289087917E-5
13	6.355177526058611E-4	1.4122616724574692E-5	3.530654181143673E-6	3.177588763029306E-5	9.532766289087917E-5
14	6.531710235115794E-4	1.4122616724574692E-5	3.530654181143673E-6	3.530654181143673E-5	1.0591962543431019E-4
15	7.202534529533092E-4	2.1183925086862037E-5	3.530654181143673E-6	3.530654181143673E-5	1.0591962543431019E-4
16	7.44968032221315E-4	2.1183925086862037E-5	3.530654181143673E-6	3.530654181143673E-5	1.2004224215888488E-4
17	7.66151957308177E-4	2.471457926800571E-5	3.530654181143673E-6	4.2367850173724074E-5	1.2004224215888488E-4
18	8.544183118367688E-4	2.8245233449149383E-5	3.530654181143673E-6	4.942915853601142E-5	1.2004224215888488E-4
19	9.144394329162113E-4	2.8245233449149383E-5	3.530654181143673E-6	5.2959812717155095E-5	1.3063420470231589E-4
20	9.426846663653606E-4	3.177588763029306E-5	3.530654181143673E-6	5.2959812717155095E-5	1.3416485888345956E-4
21	0.0010662575627053892	3.177588763029306E-5	7.061308362287346E-6	5.2959812717155095E-5	1.4122616724574692E-4
22	0.0011121560670602569	3.530654181143673E-5	7.061308362287346E-6	5.2959812717155095E-5	1.5887943815146529E-4
23	0.0011721771881396993	3.88371959925804E-5	7.061308362287346E-6	5.2959812717155095E-5	1.6594074651375262E-4
24	0.0012286676550379981	3.88371959925804E-5	7.061308362287346E-6	5.649046689829877E-5	1.800633632383273E-4
25	0.0013098727012043026	4.2367850173724074E-5	7.061308362287346E-6	5.649046689829877E-5	1.9418597996290202E-4
26	0.0013946084015517507	4.2367850173724074E-5	7.061308362287346E-6	5.649046689829877E-5	1.9418597996290202E-4
27	0.00148993606444263	4.2367850173724074E-5	7.061308362287346E-6	5.649046689829877E-5	1.9418597996290202E-4
28	0.001574671764790078	4.2367850173724074E-5	7.061308362287346E-6	5.649046689829877E-5	2.0477794250633302E-4
29	0.0017229592403981123	4.5898504354867745E-5	7.061308362287346E-6	5.649046689829877E-5	2.083085966874767E-4
30	0.0018430014825569971	4.5898504354867745E-5	7.061308362287346E-6	5.649046689829877E-5	2.224312134120514E-4
31	0.0019701050330781693	4.5898504354867745E-5	7.061308362287346E-6	5.649046689829877E-5	2.224312134120514E-4
32	0.0021995975548525083	4.5898504354867745E-5	7.061308362287346E-6	6.355177526058612E-5	2.330231759554824E-4
33	0.0023796609180908356	5.2959812717155095E-5	7.061308362287346E-6	6.355177526058612E-5	2.471457926800571E-4
34	0.0025491323187857317	5.2959812717155095E-5	7.061308362287346E-6	7.76743919851608E-5	2.471457926800571E-4
35	0.0027786248405600707	5.2959812717155095E-5	7.061308362287346E-6	8.120504616630447E-5	2.6126840940463177E-4
36	0.0029834027830664034	5.2959812717155095E-5	7.061308362287346E-6	8.120504616630447E-5	2.6479906358577545E-4
37	0.0033258762386373397	5.2959812717155095E-5	7.061308362287346E-6	8.473570034744815E-5	2.718603719480628E-4
38	0.003880188945076896	5.2959812717155095E-5	1.0591962543431018E-5	9.885831707202283E-5	2.718603719480628E-4
39	0.00408496688758323	5.2959812717155095E-5	1.0591962543431018E-5	1.0238897125316651E-4	2.718603719480628E-4
40	0.0043215207177198555	5.2959812717155095E-5	1.0591962543431018E-5	1.0591962543431019E-4	2.7892168031035016E-4
41	0.004611034360573637	5.2959812717155095E-5	1.0591962543431018E-5	1.0591962543431019E-4	2.859829886726375E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	569610	0.0	45.968594	1
AGCAGTG	579520	0.0	45.170753	2
GTGGTAT	579390	0.0	45.080296	6
AGTGGTA	579550	0.0	45.06988	5
TCAACGC	581175	0.0	44.984703	12
GTATCAA	581400	0.0	44.98225	9
CAACGCA	581530	0.0	44.957645	13
GGTATCA	581660	0.0	44.94315	8
TGGTATC	581040	0.0	44.935295	7
ATCAACG	582640	0.0	44.908295	11
ACGCAGA	582090	0.0	44.892593	15
AACGCAG	582515	0.0	44.890907	14
TATCAAC	582400	0.0	44.888065	10
GCAGTGG	585070	0.0	44.718563	3
CGCAGAG	584320	0.0	44.699547	16
AGAGTAC	577150	0.0	44.668526	19
CAGTGGT	585965	0.0	44.576065	4
CAGAGTA	581730	0.0	44.455	18
GCAGAGT	587350	0.0	44.2733	17
GAGTACT	168675	0.0	44.205215	20
>>END_MODULE