FastQCFastQC Report
Thu 26 May 2016
SRR805198_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805198_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18901599
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1680240.8889406658135113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT869000.042.7083441
AGCAGTG964050.038.5609172
TCAACGC994850.037.2702412
CAACGCA994200.037.21187613
AACGCAG1002200.036.94531614
AGTGGTA1007150.036.9107365
GCAGTGG1009800.036.8999823
GGTATCA1005600.036.8975268
ACGCAGA1004150.036.8501715
GTGGTAT1010350.036.7961546
GTATCAA1009800.036.7370769
TGGTATC1012200.036.724267
ATCAACG1011150.036.70197311
TATCAAC1015600.036.5133910
CGCAGAG1019200.036.3198616
CAGTGGT1033600.035.984374
AGAGTAC1005550.035.8663919
CAGAGTA1029650.035.20720318
GCAGAGT1053350.034.93710317
GAGTACT347900.034.6049120
Produced by FastQC (version 0.11.5)