##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805198_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18901599
Sequences flagged as poor quality	0
Sequence length	53
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.99245397175128	37.0	36.0	37.0	35.0	37.0
2	36.01563486771675	37.0	37.0	37.0	35.0	37.0
3	37.81529837766635	39.0	38.0	39.0	35.0	39.0
4	37.86131898153167	39.0	38.0	39.0	35.0	39.0
5	37.88315480610926	39.0	38.0	39.0	35.0	39.0
6	37.88262432188938	39.0	38.0	39.0	35.0	39.0
7	37.87474313681081	39.0	38.0	39.0	35.0	39.0
8	38.81966118316233	40.0	39.0	40.0	36.0	40.0
9	38.80187078352472	40.0	39.0	40.0	36.0	40.0
10	38.77630347570065	40.0	39.0	40.0	36.0	40.0
11	38.77633490161335	40.0	39.0	40.0	36.0	40.0
12	38.77229709507645	40.0	39.0	40.0	36.0	40.0
13	38.85292926804764	40.0	39.0	40.0	36.0	40.0
14	38.79016177414408	40.0	39.0	40.0	36.0	40.0
15	38.79291291705003	40.0	39.0	40.0	36.0	40.0
16	38.81171640558029	40.0	39.0	40.0	36.0	40.0
17	38.7650756425422	40.0	39.0	40.0	36.0	40.0
18	38.68972291709289	40.0	39.0	40.0	36.0	40.0
19	38.60859337879298	40.0	39.0	40.0	36.0	40.0
20	38.5869047375304	40.0	39.0	40.0	36.0	40.0
21	38.48952816108309	40.0	39.0	40.0	36.0	40.0
22	38.530897782774886	40.0	39.0	40.0	36.0	40.0
23	38.44242304579628	40.0	39.0	40.0	36.0	40.0
24	38.10560344656555	40.0	39.0	40.0	34.0	40.0
25	38.20731759254865	40.0	39.0	40.0	34.0	40.0
26	38.303912647813554	40.0	39.0	40.0	35.0	40.0
27	38.18184043582768	40.0	39.0	40.0	35.0	40.0
28	38.2960979121396	40.0	39.0	40.0	35.0	40.0
29	38.32761297073332	40.0	39.0	40.0	35.0	40.0
30	38.3197419964311	40.0	39.0	40.0	35.0	40.0
31	38.18542336021413	40.0	39.0	40.0	35.0	40.0
32	38.03407076829849	40.0	38.0	40.0	34.0	40.0
33	38.126509931778784	40.0	38.0	40.0	35.0	40.0
34	38.06861784550609	40.0	38.0	40.0	34.0	40.0
35	38.05784399510327	40.0	38.0	40.0	34.0	40.0
36	38.0009744149159	40.0	38.0	40.0	34.0	40.0
37	38.00382988761956	40.0	38.0	40.0	34.0	40.0
38	37.971793920715385	40.0	38.0	40.0	34.0	40.0
39	37.91998624031755	40.0	38.0	40.0	34.0	40.0
40	37.85902145104232	40.0	38.0	40.0	34.0	40.0
41	37.799956765562534	40.0	38.0	40.0	34.0	40.0
42	37.69869184083315	40.0	38.0	40.0	33.0	40.0
43	37.48555976666313	40.0	37.0	40.0	33.0	40.0
44	37.4165487269093	40.0	37.0	40.0	33.0	40.0
45	37.56743141149064	40.0	37.0	40.0	33.0	40.0
46	37.620288209479	40.0	37.0	40.0	33.0	40.0
47	37.60917772089017	40.0	37.0	40.0	33.0	40.0
48	37.58576901351044	40.0	37.0	40.0	33.0	40.0
49	37.426272666137926	40.0	37.0	40.0	33.0	40.0
50	37.41520196254296	40.0	37.0	40.0	33.0	40.0
51	37.05644707625	40.0	36.0	40.0	32.0	40.0
52	36.99990630422326	40.0	36.0	40.0	32.0	40.0
53	36.76086986079855	39.0	36.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	6.0
12	35.0
13	119.0
14	305.0
15	703.0
16	1674.0
17	3043.0
18	5539.0
19	9191.0
20	14116.0
21	20849.0
22	28194.0
23	37015.0
24	47093.0
25	59297.0
26	74377.0
27	91739.0
28	114256.0
29	139742.0
30	171948.0
31	210730.0
32	261353.0
33	328539.0
34	426751.0
35	586919.0
36	871088.0
37	1482082.0
38	3115564.0
39	1.0799327E7
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.871731645560782	30.5978240253642	25.235129578190712	21.2953147508843
2	23.506196486339594	27.31736611278231	28.055314262036774	21.121123138841323
3	24.810832141767477	25.524036352691642	26.567069801872318	23.098061703668563
4	22.01677752236729	27.304700517665196	26.045431394455044	24.63309056551247
5	24.022226902602263	30.403602361895416	24.87628692154563	20.69788381395669
6	26.79255866130691	25.6102830242034	26.552727099966518	21.044431214523172
7	22.52186706532077	26.13711146871754	30.158549020112	21.182472445849687
8	25.964988464732535	25.72920418002731	26.45838587518442	21.847421480055733
9	25.530697164827167	25.94730742092243	26.910035494880617	21.611959919369784
10	21.401808386687286	26.302785282874748	30.81130331883562	21.48410301160235
11	21.40778671688041	30.225310567640335	26.73413503270279	21.632767682776468
12	21.40425791489916	26.083200685825574	30.566890134533065	21.945651264742207
13	21.499323946085198	26.39348131340634	26.312387645087593	25.794807095420868
14	21.773274313987933	30.202349547252588	26.28735272608418	21.7370234126753
15	21.844696842843824	29.818159828700207	26.256318314656873	22.080825013799096
16	21.95435952270493	25.93696438063256	26.14079369687189	25.96788239979062
17	26.07471992184365	25.871287397431296	26.184551899550932	21.86944078117412
18	21.905458897948265	25.995049413544326	26.221427086671344	25.878064601836066
19	22.324523972813093	30.049214354827864	26.015746075239456	21.610515597119587
20	26.168875977106488	25.83799920842676	26.25460417396433	21.738520640502426
21	22.148332529962147	29.753831937710668	26.304219024009555	21.79361650831763
22	26.005921509603503	25.82577802015586	26.34190895701469	21.826391513225946
23	21.940239024222237	25.948455471941816	30.177610899479983	21.93369460435596
24	21.926890947162725	29.82918535093248	26.04137882726218	22.202544874642616
25	22.20305805873884	25.82272007780929	26.1941383900907	25.78008347336117
26	22.305509708464346	28.30077497676255	27.67002410748424	21.723691207288866
27	22.644004880222038	25.49673707499561	30.092337690583744	21.766920354198604
28	25.19238187203104	25.643650571573335	27.3978196236202	21.76614793277542
29	25.091284605074947	25.658850343825407	27.43851459339498	21.811350457704663
30	24.90865455351158	25.665114364133956	27.4269653059511	21.999265776403362
31	23.924208740223513	26.311747487606734	27.539347332466424	22.22469643970333
32	23.254249547882168	26.59915703428054	27.793378750654906	22.35321466718239
33	23.03715151294872	26.315075248395654	27.92694946073081	22.72082377792482
34	22.977600995555985	26.399269183522517	27.945334148714085	22.677795672207417
35	22.832951857670878	26.37163131013413	28.105886703024435	22.689530129170553
36	22.80922370641764	26.396904304233733	28.086676688041052	22.70719530130758
37	23.07011168737629	26.515555641615297	27.846813383354498	22.567519287653916
38	23.08752291274405	26.476521906956123	27.952979004580513	22.482976175719312
39	23.006159425982954	26.424039574641277	27.933388069443225	22.636412929932543
40	22.997107281770184	26.57828578418154	27.772518081671294	22.652088852376988
41	22.950349332879192	26.630942704900257	27.86772695791504	22.55098100430551
42	22.995033383154514	26.544690742830806	27.871160529857818	22.589115344156863
43	23.319032426833306	26.529438065001802	27.684620756159305	22.466908752005583
44	22.992504496577247	26.465358830223835	27.916156723037027	22.62597995016189
45	22.96851181743936	26.28490848843	27.93891141167475	22.807668282455893
46	23.01528563800343	26.451518731298872	27.768830562959252	22.76436506773845
47	22.92952569779943	26.45859220693445	27.837909374757132	22.77397272050899
48	22.86074844779005	26.415796885755537	27.8449140731427	22.87854059331171
49	23.078798783108244	26.649607792441266	27.575143245817458	22.696450178633036
50	23.02649632975496	26.61289132205164	27.655035957539887	22.70557639065351
51	23.29612431202249	26.445677955605767	27.5996226562631	22.65857507610864
52	23.150131372483354	26.599807772876783	27.51504780098234	22.735013053657525
53	23.184001522834127	26.638778020843635	27.445450514530545	22.731769941791697
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15746.0
1	10057.0
2	4368.0
3	3765.0
4	3162.0
5	3271.5
6	3381.0
7	3617.0
8	4412.5
9	4972.0
10	6307.5
11	7643.0
12	10223.0
13	12803.0
14	16196.0
15	19589.0
16	27461.0
17	35333.0
18	48298.5
19	61264.0
20	80610.5
21	99957.0
22	227105.5
23	354254.0
24	300230.0
25	276304.0
26	306402.0
27	343805.5
28	381209.0
29	417709.0
30	454209.0
31	486796.5
32	519384.0
33	565088.0
34	610792.0
35	671516.0
36	732240.0
37	795097.5
38	857955.0
39	912015.5
40	966076.0
41	1023497.5
42	1125955.0
43	1170991.0
44	1213992.5
45	1256994.0
46	1280787.0
47	1304580.0
48	1311321.0
49	1318062.0
50	1298030.0
51	1277998.0
52	1242946.5
53	1207895.0
54	1153865.0
55	1099835.0
56	1029886.5
57	959938.0
58	862681.5
59	674840.5
60	584256.0
61	508848.5
62	433441.0
63	369788.5
64	306136.0
65	250791.5
66	195447.0
67	155906.5
68	116366.0
69	90070.0
70	63774.0
71	46734.5
72	29695.0
73	22351.5
74	15008.0
75	11489.5
76	5735.5
77	3500.0
78	2440.0
79	1380.0
80	1016.5
81	653.0
82	482.0
83	311.0
84	207.5
85	104.0
86	79.5
87	55.0
88	41.0
89	27.0
90	19.5
91	12.0
92	14.0
93	10.5
94	5.0
95	3.5
96	2.0
97	2.5
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.8901599E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.95682380528129
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.14859589231301	35.32166096898409
2	15.076057444276456	14.761517759554835
3	5.0796867245006725	7.460559838722176
4	2.27244288993992	4.450063446814116
5	1.2293297669875007	3.0092040400497297
6	0.8031248371355317	2.3591064687173677
7	0.542350769579252	1.858623974686511
8	0.3978842091857186	1.5583317699207127
9	0.32451920681731267	1.4298686666626337
>10	1.9349643199807771	17.145117326733818
>50	0.12929543613535602	4.401628978085457
>100	0.060941113509837046	4.631647175775594
>500	4.928431874671065E-4	0.15616087197093337
>1k	2.2783294012519302E-4	0.21305780564183588
>5k	3.251756661356737E-5	0.10464993214706682
>10k+	5.419594435594561E-5	1.1388009755331434
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	168024	0.8889406658135113	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.406780770240656E-4	1.0581115386058078E-5	0.0	5.290557693029039E-6	1.0581115386058078E-5
2	7.618403077961817E-4	1.0581115386058078E-5	0.0	5.290557693029039E-6	2.6452788465145198E-5
3	7.830025385682978E-4	1.0581115386058078E-5	0.0	1.0581115386058078E-5	2.6452788465145198E-5
4	7.988742116473849E-4	1.0581115386058078E-5	0.0	1.587167307908712E-5	3.7033903851203276E-5
5	8.147458847264721E-4	1.0581115386058078E-5	0.0	1.587167307908712E-5	4.2324461544232314E-5
6	8.623609039637335E-4	1.587167307908712E-5	0.0	1.587167307908712E-5	5.2905576930290396E-5
7	8.623609039637335E-4	1.587167307908712E-5	0.0	1.587167307908712E-5	5.2905576930290396E-5
8	8.623609039637335E-4	1.587167307908712E-5	0.0	1.587167307908712E-5	5.819613462331943E-5
9	8.782325770428206E-4	1.587167307908712E-5	0.0	1.587167307908712E-5	6.877725000937751E-5
10	8.941042501219077E-4	1.587167307908712E-5	0.0	1.587167307908712E-5	7.93583653954356E-5
11	9.258475962800819E-4	1.587167307908712E-5	0.0	1.587167307908712E-5	1.0581115386058079E-4
12	9.41719269359169E-4	2.1162230772116157E-5	0.0	2.1162230772116157E-5	1.2168282693966791E-4
13	9.628815001312852E-4	2.1162230772116157E-5	0.0	2.6452788465145198E-5	1.5342617309784215E-4
14	9.999154039824884E-4	2.1162230772116157E-5	0.0	3.174334615817424E-5	1.6400728848390024E-4
15	0.0010104965193685465	2.1162230772116157E-5	0.0	3.174334615817424E-5	1.6400728848390024E-4
16	0.0010316587501406627	2.1162230772116157E-5	0.0	3.174334615817424E-5	1.851695192560164E-4
17	0.001068692653991866	2.1162230772116157E-5	0.0	3.7033903851203276E-5	2.010411923351035E-4
18	0.001084564327070953	2.1162230772116157E-5	0.0	3.7033903851203276E-5	2.0633175002813254E-4
19	0.0011057265578430693	2.6452788465145198E-5	0.0	4.761501923726136E-5	2.2220342310721967E-4
20	0.0011692132501594178	2.6452788465145198E-5	0.0	4.761501923726136E-5	2.4336565387933582E-4
21	0.001216828269396679	3.174334615817424E-5	0.0	4.761501923726136E-5	2.539467692653939E-4
22	0.0012803149617130276	3.174334615817424E-5	0.0	4.761501923726136E-5	2.69818442344481E-4
23	0.0013226394232572598	3.174334615817424E-5	0.0	5.819613462331943E-5	2.803995577305391E-4
24	0.0013596733271084632	3.174334615817424E-5	0.0	5.819613462331943E-5	2.856901154235681E-4
25	0.0014125789040387536	3.7033903851203276E-5	0.0	5.819613462331943E-5	2.9098067311659717E-4
26	0.001460193923276015	3.7033903851203276E-5	0.0	5.819613462331943E-5	3.0156178850265525E-4
27	0.0015554239617505377	3.7033903851203276E-5	0.0	5.819613462331943E-5	3.1214290388871334E-4
28	0.001608329538680828	3.7033903851203276E-5	0.0	5.819613462331943E-5	3.2272401927477143E-4
29	0.0016929784617692927	3.7033903851203276E-5	0.0	5.819613462331943E-5	3.280145769678005E-4
30	0.0017670462694716993	4.2324461544232314E-5	0.0	6.348669231634848E-5	3.6504848081900373E-4
31	0.0018516951925601638	5.2905576930290396E-5	0.0	6.348669231634848E-5	3.756295962050618E-4
32	0.001999830807964977	5.819613462331943E-5	0.0	6.877725000937751E-5	4.02082384670207E-4
33	0.0023701698464770097	5.819613462331943E-5	0.0	6.877725000937751E-5	4.2324461544232316E-4
34	0.0024653998849515323	5.819613462331943E-5	0.0	6.877725000937751E-5	4.3382573082838125E-4
35	0.0026135355003563455	5.819613462331943E-5	0.0	7.406780770240655E-5	4.4440684621443934E-4
36	0.0027352183272960134	5.819613462331943E-5	0.0	7.406780770240655E-5	4.549879616004974E-4
37	0.0028569011542356813	5.819613462331943E-5	0.0	7.406780770240655E-5	4.6556907698655546E-4
38	0.0029785839811753492	5.819613462331943E-5	0.0	8.464892308846463E-5	4.708596346795845E-4
39	0.0031373007119662203	5.819613462331943E-5	0.0	8.464892308846463E-5	4.8673130775867164E-4
40	0.0032748552119849753	5.819613462331943E-5	0.0	8.464892308846463E-5	4.920218654517007E-4
41	0.003454734173547963	5.819613462331943E-5	0.0	8.464892308846463E-5	4.973124231447297E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	86900	0.0	42.708344	1
AGCAGTG	96405	0.0	38.560917	2
TCAACGC	99485	0.0	37.27024	12
CAACGCA	99420	0.0	37.211876	13
AACGCAG	100220	0.0	36.945316	14
AGTGGTA	100715	0.0	36.910736	5
GCAGTGG	100980	0.0	36.899982	3
GGTATCA	100560	0.0	36.897526	8
ACGCAGA	100415	0.0	36.85017	15
GTGGTAT	101035	0.0	36.796154	6
GTATCAA	100980	0.0	36.737076	9
TGGTATC	101220	0.0	36.72426	7
ATCAACG	101115	0.0	36.701973	11
TATCAAC	101560	0.0	36.51339	10
CGCAGAG	101920	0.0	36.31986	16
CAGTGGT	103360	0.0	35.98437	4
AGAGTAC	100555	0.0	35.86639	19
CAGAGTA	102965	0.0	35.207203	18
GCAGAGT	105335	0.0	34.937103	17
GAGTACT	34790	0.0	34.60491	20
>>END_MODULE