FastQCFastQC Report
Thu 26 May 2016
SRR805197_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805197_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5880161
Sequences flagged as poor quality0
Sequence length46
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT450290.765778351987301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT188750.032.0847661
AGCAGTG210250.029.0606422
TCAACGC222750.027.21436512
CAACGCA223650.027.11379413
ATCAACG226350.026.92290711
AACGCAG225700.026.9029714
ACGCAGA226100.026.84652715
AGTGGTA226200.026.7550855
GCAGTGG228550.026.7512573
GGTATCA227400.026.5875118
GTATCAA227750.026.5817789
GTGGTAT228050.026.511736
TATCAAC228650.026.4771510
CGCAGAG229800.026.44038216
TGGTATC228450.026.3865187
AGAGTAC229400.026.16390419
CAGAGTA234400.025.5972718
CAGTGGT237350.025.5824744
GCAGAGT238550.025.4034817
GAGTACT87550.024.53455220
Produced by FastQC (version 0.11.5)