##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805197_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5880161
Sequences flagged as poor quality	0
Sequence length	46
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.579389748001795	37.0	35.0	37.0	32.0	37.0
2	35.016156870534665	37.0	35.0	37.0	32.0	37.0
3	37.01790410160538	39.0	37.0	39.0	33.0	39.0
4	37.20617190583727	39.0	37.0	39.0	34.0	39.0
5	37.309985729982564	39.0	37.0	39.0	34.0	39.0
6	37.37046927796705	39.0	37.0	39.0	34.0	39.0
7	37.33091985066395	39.0	37.0	39.0	34.0	39.0
8	38.2381378673135	40.0	38.0	40.0	34.0	40.0
9	38.29496148149685	40.0	38.0	40.0	34.0	40.0
10	38.17134530840227	40.0	38.0	40.0	34.0	40.0
11	38.22850683850323	40.0	38.0	40.0	34.0	40.0
12	38.19784203187634	40.0	38.0	40.0	34.0	40.0
13	38.28003825065334	40.0	38.0	40.0	34.0	40.0
14	38.19369860791227	40.0	38.0	40.0	34.0	40.0
15	38.15215246657362	40.0	38.0	40.0	34.0	40.0
16	38.16156734483971	40.0	38.0	40.0	34.0	40.0
17	38.22427651215673	40.0	38.0	40.0	34.0	40.0
18	38.00498846885315	40.0	38.0	40.0	34.0	40.0
19	38.046756372827204	40.0	38.0	40.0	34.0	40.0
20	37.85846237883622	40.0	38.0	40.0	34.0	40.0
21	37.88042402240347	40.0	38.0	40.0	34.0	40.0
22	37.874104807674485	40.0	38.0	40.0	34.0	40.0
23	37.85073861072851	40.0	38.0	40.0	34.0	40.0
24	37.695673808931424	40.0	38.0	40.0	33.0	40.0
25	37.74879123207681	40.0	38.0	40.0	33.0	40.0
26	37.723973544261796	40.0	38.0	40.0	33.0	40.0
27	37.65886359234042	40.0	38.0	40.0	33.0	40.0
28	37.63922773543106	40.0	38.0	40.0	33.0	40.0
29	37.590484002053685	40.0	38.0	40.0	33.0	40.0
30	37.49572639252565	40.0	38.0	40.0	33.0	40.0
31	37.499135142728235	40.0	38.0	40.0	33.0	40.0
32	37.241119758455596	40.0	37.0	40.0	31.0	40.0
33	37.31371726046277	40.0	37.0	40.0	32.0	40.0
34	37.36509816652979	40.0	37.0	40.0	32.0	40.0
35	37.30282895315281	40.0	37.0	40.0	32.0	40.0
36	37.22946718635765	40.0	37.0	40.0	32.0	40.0
37	37.166663123679776	40.0	37.0	40.0	32.0	40.0
38	37.12476886262128	40.0	37.0	40.0	31.0	40.0
39	37.09316666669501	40.0	37.0	40.0	31.0	40.0
40	36.98843518060135	40.0	37.0	40.0	31.0	40.0
41	36.707357332562836	39.0	36.0	40.0	30.0	40.0
42	35.82499424760648	39.0	35.0	40.0	27.0	40.0
43	35.95243650641539	39.0	35.0	40.0	28.0	40.0
44	36.07940275784966	39.0	35.0	40.0	29.0	40.0
45	36.271668071673545	39.0	35.0	40.0	30.0	40.0
46	36.51171762133724	39.0	36.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	21.0
10	55.0
11	101.0
12	191.0
13	384.0
14	638.0
15	1005.0
16	1470.0
17	2042.0
18	2892.0
19	4099.0
20	5719.0
21	7995.0
22	11356.0
23	15407.0
24	21169.0
25	28090.0
26	36422.0
27	46458.0
28	57713.0
29	71345.0
30	88046.0
31	105544.0
32	127770.0
33	154538.0
34	190780.0
35	243577.0
36	331517.0
37	514742.0
38	1059474.0
39	2749597.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	19.49623488200408	25.377060253962437	33.92538061457841	21.201324249455077
2	23.60493190577605	27.968043732135904	24.828316775680122	23.598707586407926
3	23.1921200797053	26.537028492927316	27.732795071427468	22.538056355939915
4	22.571048649858398	26.319670498817977	27.87859039914043	23.230690452183197
5	24.184337809798066	28.580952120188545	26.989295021003677	20.24541504900971
6	25.340020451820962	26.679796692641577	26.57381320001272	21.40636965552474
7	23.070932921734627	26.402287284310756	30.00603214775922	20.520747646195403
8	24.917872146698024	26.45735380374789	26.667552129950185	21.957221919603903
9	24.397699314695632	26.42948041728789	27.30202455340934	21.870795714607134
10	22.19502153087305	26.443918797461496	29.493172040697523	21.867887630967928
11	21.71789173799833	29.03196017932162	27.14260714970219	22.107540932977855
12	21.801477884704177	26.923106357121856	29.380862190678116	21.894553567495855
13	21.64396519074903	26.68624209439163	27.07788103080851	24.591911684050828
14	21.975724814337568	29.106754049761562	26.739624986458704	22.177896149442166
15	22.108272205471923	28.95930910735267	26.888889606934228	22.04352908024117
16	21.899332348212912	26.671565625499028	26.774130844376543	24.654971181911517
17	24.52995759809978	26.372356131065118	26.889875974484372	22.20781029635073
18	22.614737929794778	26.271100400142107	26.869553401684072	24.244608268379046
19	22.148849325724246	28.86738985548185	26.840710654011	22.143050164782903
20	24.810068976002526	26.371709890256405	26.781851721406944	22.036369412334118
21	22.298658148986057	28.7521889281603	26.98113197920941	21.96802094364423
22	24.594190533218395	26.488934571689448	26.84217319899914	22.074701696093015
23	22.33192254429768	26.29159303631312	29.07755416901	22.298930250379197
24	22.130397450001794	28.756168411035006	26.93472168534161	22.178712453621593
25	22.26362509461901	26.369618110796626	26.849451911265692	24.517304883318673
26	22.609414946291437	27.620145094666626	27.467989396888964	22.302450562152977
27	22.578038254394738	26.15805927762862	29.25321262462031	22.010689843356328
28	24.296613647143335	26.013947577285723	27.69728583962242	21.99215293594852
29	24.26530498059492	26.10413218277527	27.517426818755474	22.11313601787434
30	24.14416884163546	26.092414816533083	27.582782172120794	22.180634169710657
31	23.24681245972687	26.563337296376748	27.87054640170567	22.319303842190717
32	23.13812495950366	26.633930601560056	27.915613194944832	22.312331243991448
33	22.655842246496313	26.65188929350744	28.097342912889626	22.594925547106616
34	22.659209501236447	26.626651889293505	28.128770623797543	22.585367985672498
35	22.75874759211525	26.65949112617835	27.96600297168734	22.615758310019064
36	22.641692974052923	26.674082563385593	28.069979716541777	22.61424474601971
37	22.75294843117391	26.636770659850978	28.040711810441927	22.569569098533186
38	22.867299041641886	26.675477083025445	27.874695947951082	22.58252792738158
39	22.75641772393647	26.637961103445978	27.968502903236832	22.637118269380718
40	22.775992017905633	26.64071613005154	27.886345288844982	22.696946563197844
41	22.84323507468588	26.691888198299335	27.803252325914208	22.661624401100582
42	22.87763889458129	26.802769516004748	27.518515224328045	22.801076365085923
43	22.902094007289936	26.58605776270412	27.906599836296998	22.605248393708948
44	23.065184779804497	26.60484976516799	27.77122939320879	22.558736061818717
45	22.740482786100586	26.53131436367133	27.94622460167332	22.78197824855476
46	22.838082154553252	26.595292203733877	27.894440305290964	22.67218533642191
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1798.0
1	1206.0
2	614.0
3	528.0
4	442.0
5	577.5
6	713.0
7	713.0
8	1021.0
9	1329.0
10	2024.0
11	2719.0
12	4105.0
13	5491.0
14	7849.0
15	10207.0
16	14686.0
17	19165.0
18	26181.5
19	33198.0
20	33198.0
21	43140.5
22	53083.0
23	66557.5
24	80032.0
25	123283.5
26	166535.0
27	155036.0
28	143537.0
29	157345.5
30	171154.0
31	171154.0
32	191382.0
33	211610.0
34	232631.0
35	253652.0
36	276178.0
37	298704.0
38	321402.5
39	344101.0
40	365729.0
41	387357.0
42	408340.0
43	429323.0
44	429323.0
45	444522.5
46	459722.0
47	465610.5
48	471499.0
49	465775.0
50	460051.0
51	444334.0
52	428617.0
53	404262.5
54	379908.0
55	349088.0
56	318268.0
57	318268.0
58	284110.5
59	249953.0
60	217560.0
61	185167.0
62	157800.0
63	130433.0
64	107177.5
65	83922.0
66	67137.0
67	50352.0
68	38221.5
69	26091.0
70	26091.0
71	19112.5
72	12134.0
73	8758.5
74	5383.0
75	3894.5
76	2406.0
77	1646.0
78	886.0
79	591.0
80	296.0
81	296.0
82	219.5
83	143.0
84	109.5
85	76.0
86	69.5
87	63.0
88	38.5
89	14.0
90	10.5
91	7.0
92	5.5
93	4.0
94	4.0
95	2.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	5880161.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.71025230822214
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.66421710151582	52.47709770470883
2	13.449665818105572	17.944612003741874
3	3.7145004063242593	7.43385777914655
4	1.4213488819529192	3.792741701323547
5	0.7832991311524394	2.6127041335995207
6	0.4858978328507362	1.9448620215294574
7	0.3419382304417949	1.5967549938619399
8	0.21867894792110512	1.1670502232250792
9	0.17039134534087488	1.0230164674944455
>10	0.7346943173378524	8.11310893395638
>50	0.01335427627729838	0.586489518015797
>100	0.001775157198893047	0.17640769837309497
>500	5.3013606649640437E-5	0.019946100014657544
>1k	1.3252855267415246E-4	0.22670126005546226
>5k	2.6505710518118856E-5	0.08844549586634576
>10k+	2.6505710518118856E-5	0.7962039650871018
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	45029	0.765778351987301	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.401267414276582E-4	0.0	0.0	0.0	1.700633707138291E-5
2	4.0815208971318984E-4	0.0	0.0	1.700633707138291E-5	1.700633707138291E-5
3	4.251584267845727E-4	0.0	0.0	1.700633707138291E-5	1.700633707138291E-5
4	4.4216476385595564E-4	0.0	0.0	1.700633707138291E-5	1.700633707138291E-5
5	0.0010203802242829746	0.0	0.0	1.700633707138291E-5	1.700633707138291E-5
6	0.0010713992354971233	0.0	0.0	1.700633707138291E-5	5.101901121414873E-5
7	0.0010884055725685063	0.0	0.0	1.700633707138291E-5	5.101901121414873E-5
8	0.0010884055725685063	0.0	0.0	1.700633707138291E-5	6.802534828553164E-5
9	0.0011054119096398892	0.0	0.0	1.700633707138291E-5	6.802534828553164E-5
10	0.0011904435949968036	0.0	0.0	1.700633707138291E-5	6.802534828553164E-5
11	0.0012244562691395694	0.0	0.0	1.700633707138291E-5	8.503168535691455E-5
12	0.0013605069657106328	0.0	0.0	1.700633707138291E-5	1.1904435949968037E-4
13	0.0014285323139961643	0.0	0.0	1.700633707138291E-5	1.3605069657106328E-4
14	0.0014625449881389301	0.0	0.0	1.700633707138291E-5	1.3605069657106328E-4
15	0.001496557662281696	0.0	0.0	3.401267414276582E-5	1.3605069657106328E-4
16	0.0015305703364244618	0.0	0.0	3.401267414276582E-5	1.700633707138291E-4
17	0.0015815893476386106	0.0	0.0	3.401267414276582E-5	1.700633707138291E-4
18	0.0015985956847099935	0.0	0.0	3.401267414276582E-5	1.700633707138291E-4
19	0.0016326083588527594	0.0	0.0	3.401267414276582E-5	1.700633707138291E-4
20	0.001683627370066908	0.0	0.0	3.401267414276582E-5	1.87069707785212E-4
21	0.0017176400442096738	0.0	0.0	5.101901121414873E-5	1.87069707785212E-4
22	0.0017686590554238225	1.700633707138291E-5	0.0	5.101901121414873E-5	1.87069707785212E-4
23	0.0018196780666379713	1.700633707138291E-5	0.0	5.101901121414873E-5	1.87069707785212E-4
24	0.001904709751994886	1.700633707138291E-5	0.0	5.101901121414873E-5	1.87069707785212E-4
25	0.0019557287632090345	1.700633707138291E-5	0.0	5.101901121414873E-5	1.87069707785212E-4
26	0.002023754111494566	1.700633707138291E-5	0.0	5.101901121414873E-5	2.0407604485659492E-4
27	0.002108785796851481	1.700633707138291E-5	0.0	5.101901121414873E-5	2.0407604485659492E-4
28	0.0021427984709942467	1.700633707138291E-5	0.0	5.101901121414873E-5	2.2108238192797782E-4
29	0.0021938174822083954	1.700633707138291E-5	0.0	5.101901121414873E-5	2.2108238192797782E-4
30	0.002295855504636693	1.700633707138291E-5	0.0	5.101901121414873E-5	2.2108238192797782E-4
31	0.002380887189993607	1.700633707138291E-5	0.0	6.802534828553164E-5	2.2108238192797782E-4
32	0.002431906201207756	1.700633707138291E-5	0.0	6.802534828553164E-5	2.2108238192797782E-4
33	0.0024659188753505218	1.700633707138291E-5	0.0	6.802534828553164E-5	2.5509505607074364E-4
34	0.0025509505607074364	1.700633707138291E-5	0.0	1.0203802242829746E-4	2.5509505607074364E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	18875	0.0	32.084766	1
AGCAGTG	21025	0.0	29.060642	2
TCAACGC	22275	0.0	27.214365	12
CAACGCA	22365	0.0	27.113794	13
ATCAACG	22635	0.0	26.922907	11
AACGCAG	22570	0.0	26.90297	14
ACGCAGA	22610	0.0	26.846527	15
AGTGGTA	22620	0.0	26.755085	5
GCAGTGG	22855	0.0	26.751257	3
GGTATCA	22740	0.0	26.587511	8
GTATCAA	22775	0.0	26.581778	9
GTGGTAT	22805	0.0	26.51173	6
TATCAAC	22865	0.0	26.47715	10
CGCAGAG	22980	0.0	26.440382	16
TGGTATC	22845	0.0	26.386518	7
AGAGTAC	22940	0.0	26.163904	19
CAGAGTA	23440	0.0	25.59727	18
CAGTGGT	23735	0.0	25.582474	4
GCAGAGT	23855	0.0	25.40348	17
GAGTACT	8755	0.0	24.534552	20
>>END_MODULE