FastQCFastQC Report
Thu 26 May 2016
SRR805196_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805196_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13863044
Sequences flagged as poor quality0
Sequence length46
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT3500832.525296753007493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT994250.038.213731
AGCAGTG1045950.036.315312
TCAACGC1076950.035.03969212
GCAGTGG1085500.034.986643
CAACGCA1079750.034.97105813
AACGCAG1084700.034.8206914
ACGCAGA1085200.034.80095715
AGTGGTA1086600.034.7855725
GGTATCA1087050.034.7840468
GTGGTAT1086850.034.740766
GTATCAA1088350.034.6579679
TGGTATC1089750.034.6373027
ATCAACG1093450.034.62984511
CGCAGAG1096350.034.5510116
TATCAAC1093050.034.4558810
CAGTGGT1098500.034.386894
GAGTACT460350.034.31302320
AGAGTAC1096100.034.26329819
CAGAGTA1105950.033.96898718
GCAGAGT1120350.033.75909417
Produced by FastQC (version 0.11.5)