##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805196_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13863044
Sequences flagged as poor quality	0
Sequence length	46
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.13961219483975	37.0	35.0	37.0	35.0	37.0
2	36.12124992173436	37.0	35.0	37.0	35.0	37.0
3	37.965359195282076	39.0	38.0	39.0	35.0	39.0
4	37.93051410642569	39.0	38.0	39.0	35.0	39.0
5	37.91779042178615	39.0	38.0	39.0	35.0	39.0
6	37.90874695341081	39.0	38.0	39.0	35.0	39.0
7	37.81379774889267	39.0	38.0	39.0	35.0	39.0
8	38.8386741757438	40.0	39.0	40.0	36.0	40.0
9	38.837216703633054	40.0	39.0	40.0	36.0	40.0
10	38.635929670280206	40.0	39.0	40.0	36.0	40.0
11	38.79825130757718	40.0	39.0	40.0	36.0	40.0
12	38.79184723066594	40.0	39.0	40.0	36.0	40.0
13	38.85717004144256	40.0	39.0	40.0	36.0	40.0
14	38.816177384995676	40.0	39.0	40.0	36.0	40.0
15	38.71233302007842	40.0	39.0	40.0	36.0	40.0
16	38.73629976215902	40.0	39.0	40.0	36.0	40.0
17	38.772154730230966	40.0	39.0	40.0	36.0	40.0
18	38.658107988404275	40.0	39.0	40.0	36.0	40.0
19	38.69628820337005	40.0	39.0	40.0	36.0	40.0
20	38.560379884821835	40.0	39.0	40.0	36.0	40.0
21	38.56329576678831	40.0	39.0	40.0	36.0	40.0
22	38.550704520594465	40.0	39.0	40.0	36.0	40.0
23	38.4332835559059	40.0	39.0	40.0	36.0	40.0
24	38.374853964252004	40.0	38.0	40.0	35.0	40.0
25	38.42909616387281	40.0	39.0	40.0	35.0	40.0
26	38.42350734802544	40.0	39.0	40.0	35.0	40.0
27	38.358367181118375	40.0	38.0	40.0	35.0	40.0
28	38.31746094147865	40.0	38.0	40.0	35.0	40.0
29	38.27981769371864	40.0	38.0	40.0	35.0	40.0
30	38.13392260747351	40.0	38.0	40.0	35.0	40.0
31	38.18838957735401	40.0	38.0	40.0	35.0	40.0
32	37.9246496656867	40.0	38.0	40.0	34.0	40.0
33	37.99239827847333	40.0	38.0	40.0	34.0	40.0
34	38.03133193546814	40.0	38.0	40.0	34.0	40.0
35	38.00714554465816	40.0	38.0	40.0	34.0	40.0
36	37.90310656158921	40.0	38.0	40.0	34.0	40.0
37	37.815640778461066	40.0	38.0	40.0	34.0	40.0
38	37.735012526830324	40.0	37.0	40.0	34.0	40.0
39	37.721940722398344	40.0	37.0	40.0	33.0	40.0
40	37.67285460538104	40.0	37.0	40.0	33.0	40.0
41	37.46290425104328	40.0	37.0	40.0	33.0	40.0
42	36.930394147201724	40.0	36.0	40.0	32.0	40.0
43	36.93994955220513	39.0	36.0	40.0	31.0	40.0
44	36.983538896652135	39.0	36.0	40.0	32.0	40.0
45	37.05373495171767	39.0	36.0	40.0	32.0	40.0
46	37.268796376899616	40.0	37.0	40.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	12.0
14	49.0
15	137.0
16	391.0
17	783.0
18	1304.0
19	1915.0
20	3068.0
21	5188.0
22	8616.0
23	13671.0
24	20657.0
25	30229.0
26	42636.0
27	57784.0
28	76948.0
29	99335.0
30	126879.0
31	160597.0
32	201576.0
33	255213.0
34	329276.0
35	446413.0
36	641657.0
37	1086627.0
38	2597756.0
39	7654324.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.292105831879347	30.801965282660866	24.390076234339297	23.51585265112049
2	21.74108370427159	31.56699928240868	23.364998336584662	23.32691867673507
3	26.00549345439573	24.948755843233275	26.993169754059785	22.052580948311206
4	20.954979296033397	24.880177831073752	27.232561622108392	26.932281250784463
5	22.78505355678017	31.583727210272144	26.35965088186981	19.27156835107787
6	28.271179114774505	25.319670052262694	26.204829184701428	20.204321648261377
7	22.162672209653234	25.648493938272143	32.717028092820016	19.471805759254604
8	28.206792101359557	25.73036628896222	25.54627973481149	20.51656187486673
9	27.380977799680934	25.456797222889865	26.44827499645821	20.71394998097099
10	20.953074952369768	25.456631314161594	32.77769297998333	20.81260075348531
11	20.712146625228918	32.39973125671389	25.80314972671226	21.08497239134493
12	20.704009884120687	25.68645096993128	32.640147430823994	20.96939171512404
13	20.687779682442038	25.91829759755505	25.538388250084182	27.85553446991873
14	20.94885509993332	32.71475586458501	25.552245235606264	20.78414379987541
15	21.121919543788508	32.19776984044774	25.78055007255261	20.899760543211144
16	20.85471271677418	25.57489538372669	25.672824813944185	27.897567085554947
17	27.781308347575035	25.591002957214883	25.789624558646718	20.838064136563368
18	21.051725724884086	25.47434748097171	25.916811632423588	27.557115161720613
19	21.08893977397749	32.41778645440352	25.71936581893558	20.773907952683405
20	28.059349735887736	25.322728543601247	25.770667683086053	20.84725403742497
21	20.946214987126925	32.203259255326614	25.968726637526363	20.881799120020105
22	27.82967434857741	25.3635709444477	25.795799248707567	21.01095545826732
23	21.008264851500147	25.50850303872656	32.370935272224486	21.11229683754881
24	20.94652516431456	32.21661851466388	25.72429980024589	21.11255652077567
25	21.15104013231149	25.34917295220299	25.761384007725862	27.738402907759653
26	21.414503192805274	29.249268775313702	28.456506377675783	20.879721654205238
27	21.42473182657431	25.19872258935339	32.479821891930804	20.896723692141496
28	25.502241787590087	25.220680248868867	28.414798366073136	20.862279597467914
29	25.353378377793508	25.329905899454698	28.4070727900741	20.9096429326777
30	25.2278143241845	25.27085681903628	28.526317885162882	20.975010971616335
31	23.428945331198545	26.062140464965704	28.945403332774532	21.56351087106122
32	22.68206751706191	26.58004980724291	29.21628179207972	21.52160088361546
33	22.186995871902305	26.283715178282634	29.56062175089396	21.968667198921104
34	22.152032410775007	26.31378793863743	29.499206667741944	22.034972982845616
35	22.06433882774952	26.334353407520023	29.553502102424257	22.0478056623062
36	21.941515874868465	26.38195478568776	29.637105674626728	22.039423664817047
37	22.22765793717455	26.367484659213375	29.411801621635192	21.993055781976885
38	22.346390879232583	26.426916051049105	29.338181426820835	21.888511642897477
39	22.19611363853422	26.35732815967402	29.432143474405763	22.014414727385994
40	22.242293972377205	26.413123986333737	29.263212321911407	22.08136971937765
41	22.252868850448717	26.555336620153554	29.259461341967896	21.93233318742983
42	22.309927026127884	26.663509110986016	29.187334325707976	21.839229537178127
43	22.32920850572212	26.399771940419438	29.35685697888573	21.91416257497271
44	22.409667025510416	26.411399978244315	29.25747043722865	21.921462559016618
45	22.168789192330344	26.29208274892585	29.491199768247146	22.047928290496664
46	22.298753433950004	26.338443418343044	29.230066643372123	22.132736504334837
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5954.0
1	3998.0
2	2042.0
3	1732.5
4	1423.0
5	1592.0
6	1761.0
7	1761.0
8	2426.5
9	3092.0
10	4418.0
11	5744.0
12	9144.0
13	12544.0
14	17442.5
15	22341.0
16	32537.5
17	42734.0
18	59900.5
19	77067.0
20	77067.0
21	101974.5
22	126882.0
23	161341.5
24	195801.0
25	418083.5
26	640366.0
27	499839.0
28	359312.0
29	385653.5
30	411995.0
31	411995.0
32	456175.5
33	500356.0
34	549858.0
35	599360.0
36	652189.5
37	705019.0
38	759723.0
39	814427.0
40	858603.5
41	902780.0
42	943204.5
43	983629.0
44	983629.0
45	1015021.5
46	1046414.0
47	1059015.0
48	1071616.0
49	1056601.0
50	1041586.0
51	1004169.5
52	966753.0
53	911234.5
54	855716.0
55	795332.0
56	734948.0
57	734948.0
58	653180.5
59	571413.0
60	496274.5
61	421136.0
62	359567.0
63	297998.0
64	247442.0
65	196886.0
66	159428.5
67	121971.0
68	93257.5
69	64544.0
70	64544.0
71	47724.5
72	30905.0
73	22919.5
74	14934.0
75	11143.5
76	7353.0
77	4985.5
78	2618.0
79	1701.0
80	784.0
81	784.0
82	625.0
83	466.0
84	333.5
85	201.0
86	168.5
87	136.0
88	76.5
89	17.0
90	14.0
91	11.0
92	8.0
93	5.0
94	5.0
95	4.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1.3863044E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.6115589163828
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.21714762463112	32.263191545294596
2	16.955721010438747	15.806651776956308
3	5.508402152287204	7.702656343693945
4	2.4383602684625223	4.546230932512314
5	1.3179960544747813	3.0716925372355677
6	0.8322555521054711	2.3275637220270515
7	0.5778318875897872	1.8853551550509235
8	0.42496570430951236	1.5846651171091957
9	0.35542071928771296	1.4910042417464147
>10	2.1790830545425823	17.71027221135476
>50	0.1363796886078381	4.388951930178258
>100	0.05551431957411437	3.8824546307528194
>500	5.417043938106048E-4	0.17572418742473125
>1k	2.6935301030379645E-4	0.21606125198214507
>5k	6.337501971477969E-5	0.18209985184109911
>10k+	4.753126478608477E-5	2.7654245648400253
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	350083	2.525296753007493	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0026256859604571695	0.0	0.0	0.0	7.213422968288927E-6
2	0.002683393344203481	0.0	0.0	7.213422968288927E-6	7.213422968288927E-6
3	0.0027122470360766366	0.0	0.0	7.213422968288927E-6	7.213422968288927E-6
4	0.00275552757388637	0.0	0.0	1.4426845936577854E-5	1.4426845936577854E-5
5	0.004768072582038981	0.0	0.0	1.4426845936577854E-5	2.164026890486678E-5
6	0.004847420234690159	0.0	0.0	2.8853691873155708E-5	2.164026890486678E-5
7	0.004847420234690159	0.0	0.0	2.8853691873155708E-5	2.8853691873155708E-5
8	0.004854633657658448	0.0	0.0	2.8853691873155708E-5	2.8853691873155708E-5
9	0.004869060503595026	0.0	0.0	2.8853691873155708E-5	3.6067114841444634E-5
10	0.004883487349531604	0.0	0.0	3.6067114841444634E-5	5.049396077802249E-5
11	0.005092676615611982	0.0	0.0	3.6067114841444634E-5	5.049396077802249E-5
12	0.005172024268263161	0.0	0.0	3.6067114841444634E-5	5.049396077802249E-5
13	0.005424494072153273	0.0	0.0	4.328053780973356E-5	5.7707383746311415E-5
14	0.005496628301836163	0.0	0.0	4.328053780973356E-5	6.492080671460035E-5
15	0.0055254819937093184	0.0	0.0	4.328053780973356E-5	7.93476526511782E-5
16	0.005554335685582474	0.0	0.0	4.328053780973356E-5	9.377449858775606E-5
17	0.00558318937745563	0.0	0.0	4.328053780973356E-5	9.377449858775606E-5
18	0.005597616223392207	0.0	0.0	4.328053780973356E-5	9.377449858775606E-5
19	0.005604829646360496	0.0	0.0	5.7707383746311415E-5	9.377449858775606E-5
20	0.005619256492297074	0.0	0.0	5.7707383746311415E-5	9.377449858775606E-5
21	0.005662537030106808	0.0	0.0	5.7707383746311415E-5	1.0820134452433391E-4
22	0.005684177299011674	0.0	0.0	5.7707383746311415E-5	1.0820134452433391E-4
23	0.005684177299011674	0.0	0.0	6.492080671460035E-5	1.2262819046091175E-4
24	0.0057202444138531195	0.0	0.0	6.492080671460035E-5	1.2262819046091175E-4
25	0.005763524951662853	0.0	0.0	7.213422968288927E-5	1.298416134292007E-4
26	0.005821232335409164	0.0	0.0	7.93476526511782E-5	1.3705503639748962E-4
27	0.00585008602728232	0.0	0.0	7.93476526511782E-5	1.3705503639748962E-4
28	0.005951073948838365	7.213422968288927E-6	0.0	7.93476526511782E-5	1.3705503639748962E-4
29	0.00611698267710901	7.213422968288927E-6	0.0	7.93476526511782E-5	1.3705503639748962E-4
30	0.006275677982411366	7.213422968288927E-6	0.0	7.93476526511782E-5	1.3705503639748962E-4
31	0.0063766659039674115	7.213422968288927E-6	0.0	1.0098792155604498E-4	1.3705503639748962E-4
32	0.0064632269795868785	7.213422968288927E-6	0.0	1.0098792155604498E-4	1.5148188233406746E-4
33	0.0066579893997306796	7.213422968288927E-6	0.0	1.0098792155604498E-4	1.586953053023564E-4
34	0.007170142430479193	7.213422968288927E-6	0.0	1.0820134452433391E-4	1.586953053023564E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	99425	0.0	38.21373	1
AGCAGTG	104595	0.0	36.31531	2
TCAACGC	107695	0.0	35.039692	12
GCAGTGG	108550	0.0	34.98664	3
CAACGCA	107975	0.0	34.971058	13
AACGCAG	108470	0.0	34.82069	14
ACGCAGA	108520	0.0	34.800957	15
AGTGGTA	108660	0.0	34.785572	5
GGTATCA	108705	0.0	34.784046	8
GTGGTAT	108685	0.0	34.74076	6
GTATCAA	108835	0.0	34.657967	9
TGGTATC	108975	0.0	34.637302	7
ATCAACG	109345	0.0	34.629845	11
CGCAGAG	109635	0.0	34.55101	16
TATCAAC	109305	0.0	34.45588	10
CAGTGGT	109850	0.0	34.38689	4
GAGTACT	46035	0.0	34.313023	20
AGAGTAC	109610	0.0	34.263298	19
CAGAGTA	110595	0.0	33.968987	18
GCAGAGT	112035	0.0	33.759094	17
>>END_MODULE