FastQCFastQC Report
Thu 26 May 2016
SRR805195_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805195_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14968197
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT5406783.612178540942506No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGC349040.23318773797538878No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC304180.2032175284705299No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGG275860.18429741404392258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2245150.038.8508571
AGCAGTG2291800.038.0434572
GTGGTAT2289000.037.8628236
TCAACGC2294050.037.85357712
AGTGGTA2291600.037.8416825
CAACGCA2297050.037.8163313
ATCAACG2307950.037.74691811
GTATCAA2297800.037.7308739
GGTATCA2298450.037.7228138
AACGCAG2304550.037.71929614
ACGCAGA2306150.037.68618815
TGGTATC2299000.037.6685527
TATCAAC2301850.037.64537410
GCAGTGG2316850.037.6148643
CGCAGAG2322650.037.46238316
CAGTGGT2314950.037.456534
AGAGTAC2301950.037.43782419
CAGAGTA2317000.037.20500618
GCAGAGT2336000.037.14126617
GTACATG1645400.037.0037722