##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805195_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14968197
Sequences flagged as poor quality	0
Sequence length	46
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.714149473046085	37.0	35.0	37.0	35.0	37.0
2	36.02436292093162	37.0	35.0	37.0	35.0	37.0
3	37.93455798316925	39.0	39.0	39.0	35.0	39.0
4	37.92374011378926	39.0	38.0	39.0	35.0	39.0
5	37.90357923536148	39.0	38.0	39.0	35.0	39.0
6	37.933153137949745	39.0	38.0	39.0	35.0	39.0
7	37.81027447728006	39.0	38.0	39.0	35.0	39.0
8	38.72353637515594	40.0	39.0	40.0	36.0	40.0
9	38.78994724615129	40.0	39.0	40.0	36.0	40.0
10	38.48762459499965	40.0	39.0	40.0	36.0	40.0
11	38.69246456336725	40.0	39.0	40.0	36.0	40.0
12	38.74181907146198	40.0	39.0	40.0	36.0	40.0
13	38.83452035004617	40.0	39.0	40.0	36.0	40.0
14	38.774926666184314	40.0	39.0	40.0	36.0	40.0
15	38.74705724410228	40.0	39.0	40.0	36.0	40.0
16	38.719583995320214	40.0	39.0	40.0	36.0	40.0
17	38.75366866162972	40.0	39.0	40.0	36.0	40.0
18	38.65889946531302	40.0	39.0	40.0	36.0	40.0
19	38.66062572532951	40.0	39.0	40.0	36.0	40.0
20	38.55822969192616	40.0	39.0	40.0	36.0	40.0
21	38.502373665979945	40.0	39.0	40.0	36.0	40.0
22	38.517486641844705	40.0	39.0	40.0	36.0	40.0
23	38.30695173239636	40.0	38.0	40.0	35.0	40.0
24	38.27110493000593	40.0	38.0	40.0	35.0	40.0
25	38.38286167666019	40.0	38.0	40.0	35.0	40.0
26	38.36100192962452	40.0	38.0	40.0	35.0	40.0
27	38.320680774043794	40.0	38.0	40.0	35.0	40.0
28	38.27846607042919	40.0	38.0	40.0	35.0	40.0
29	38.2333208869445	40.0	38.0	40.0	35.0	40.0
30	38.16111439473973	40.0	38.0	40.0	35.0	40.0
31	38.129233267039446	40.0	38.0	40.0	34.0	40.0
32	37.728291523688526	40.0	38.0	40.0	33.0	40.0
33	37.80396984352892	40.0	38.0	40.0	33.0	40.0
34	37.873393034578584	40.0	38.0	40.0	34.0	40.0
35	37.86262740929986	40.0	38.0	40.0	34.0	40.0
36	37.76424401683115	40.0	38.0	40.0	33.0	40.0
37	37.66531366469856	40.0	37.0	40.0	33.0	40.0
38	37.582245810901604	40.0	37.0	40.0	33.0	40.0
39	37.55045353825848	40.0	37.0	40.0	33.0	40.0
40	37.47852703969623	40.0	37.0	40.0	33.0	40.0
41	37.301128385736774	40.0	36.0	40.0	33.0	40.0
42	36.76847832775049	39.0	36.0	40.0	31.0	40.0
43	36.7670877127018	39.0	35.0	40.0	31.0	40.0
44	36.78447317335548	39.0	35.0	40.0	31.0	40.0
45	36.83151477763153	39.0	35.0	40.0	32.0	40.0
46	36.983422385475016	39.0	36.0	40.0	32.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	6.0
12	17.0
13	39.0
14	82.0
15	238.0
16	489.0
17	972.0
18	1706.0
19	2796.0
20	4306.0
21	6980.0
22	11397.0
23	17611.0
24	26259.0
25	37654.0
26	52167.0
27	70772.0
28	92379.0
29	117880.0
30	148954.0
31	185782.0
32	230861.0
33	286454.0
34	366642.0
35	493256.0
36	709852.0
37	1232802.0
38	2907706.0
39	7962138.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.286356800354778	36.72263265909715	28.76077860279364	19.23023193775443
2	23.084203127470865	39.605090713330405	18.279496187817408	19.031209971381323
3	37.2699998536898	22.84510285373716	19.743747359818954	20.141149932754093
4	21.553785001627116	21.880310634607493	20.46995372923005	36.09595063453534
5	21.388928806856295	36.78272673722827	21.73439459675738	20.09394985915805
6	36.109051744842745	22.295878388024956	20.689064955518692	20.906004911613604
7	21.486562476429192	21.817049842409208	36.60565130189027	20.09073637927133
8	35.27823691791336	22.84832301445525	21.797027390807322	20.076412676824066
9	35.02006955146301	22.56228990038012	22.12997330272978	20.287667245427087
10	20.48825252633968	22.633520924397242	36.70577024073107	20.172456308532016
11	20.20573352956271	37.07827335516763	22.380157075698563	20.3358360395711
12	20.146735107775505	22.691777773902896	36.756076900911985	20.40541021740962
13	20.029232645722125	22.726377799543926	22.448187981491692	34.79620157324225
14	20.3281864876578	37.145422391220535	22.1922252893919	20.334165831729766
15	20.336597654346747	36.90175242883294	22.245524962024486	20.516124954795824
16	20.30204439452527	22.423609202898653	22.39877655271373	34.875569849862345
17	34.954597404082804	22.25557293239794	22.331493900033518	20.458335763485742
18	20.603116060003753	22.340973999740918	22.309193284936054	34.746716655319275
19	20.466880546801995	36.904651909645494	22.33918353693501	20.289284006617496
20	35.09915055233439	22.358183821338002	22.36547260835757	20.177193017970033
21	20.55519445662026	36.7197331782846	22.375767769491542	20.3493045956036
22	34.82742109821243	22.275020832502403	22.570146558065744	20.32741151121942
23	20.52195063974639	22.317744749083673	36.796482569009484	20.363822042160457
24	20.49914896229653	36.573255950599794	22.353373622754965	20.574221464348714
25	20.38856116070626	22.39556975365837	22.481438479196928	34.73443060643844
26	20.724920977456403	32.371153319267506	26.508022308899328	20.39590339437676
27	20.880637794919455	22.190468230742823	36.61298685472939	20.315907119608326
28	31.02769825918245	22.13068815168587	26.55269034740791	20.28892324172377
29	31.14842088195392	22.191310015494853	26.36561370751601	20.29465539503522
30	30.93696588840994	22.19199814112548	26.389885167866247	20.481150802598336
31	26.98798659584718	23.741496721348604	27.429148614225213	21.841368068579
32	24.645800693296593	25.41662165456534	27.966628178397173	21.97094947374089
33	23.969580304160882	24.592748211424528	28.556091291422742	22.881580192991848
34	23.37878770569361	24.378199992958404	29.093350388159646	23.14966191318834
35	23.130354310542543	24.518303707520687	29.142514626177086	23.20882735575968
36	23.06454812159407	24.67743442981142	29.186634836513708	23.0713826120808
37	23.700342800138184	24.42616168133009	29.067549017426746	22.805946501104977
38	23.922520528023515	24.512457980076025	28.905211496080657	22.659809995819806
39	23.79529077550222	24.45804260860543	28.93029801785746	22.816368598034884
40	23.352445187620123	24.793059578251142	28.72180931343969	23.132685920689045
41	23.591565503847924	25.18897232579181	28.462546290645424	22.756915879714835
42	23.74865189174087	25.17950558774714	28.467663807471265	22.604178713040724
43	23.6227850288181	24.7643186417175	29.08221344227364	22.530682887190757
44	23.770618465270065	24.59932214948801	28.686026780646994	22.94403260459493
45	23.634576696177902	24.30753015877597	28.72053995548028	23.337353189565853
46	23.538065406274384	24.421445014386165	28.585005929571878	23.45548364976757
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2914.0
1	1894.5
2	875.0
3	777.5
4	680.0
5	777.0
6	874.0
7	874.0
8	1235.0
9	1596.0
10	2295.0
11	2994.0
12	4828.0
13	6662.0
14	9483.5
15	12305.0
16	17625.5
17	22946.0
18	33496.0
19	44046.0
20	44046.0
21	58062.5
22	72079.0
23	95605.0
24	119131.0
25	424803.0
26	730475.0
27	491323.5
28	252172.0
29	263131.5
30	274091.0
31	274091.0
32	310251.0
33	346411.0
34	390153.5
35	433896.0
36	486432.0
37	538968.0
38	598401.5
39	657835.0
40	723942.0
41	790049.0
42	869768.5
43	949488.0
44	949488.0
45	1042163.0
46	1134838.0
47	1193038.5
48	1251239.0
49	1290569.5
50	1329900.0
51	1311782.5
52	1293665.0
53	1241773.0
54	1189881.0
55	1134803.5
56	1079726.0
57	1079726.0
58	936363.5
59	793001.0
60	689612.0
61	586223.0
62	501211.0
63	416199.0
64	346351.5
65	276504.0
66	226598.5
67	176693.0
68	135469.0
69	94245.0
70	94245.0
71	70168.5
72	46092.0
73	33651.0
74	21210.0
75	16034.0
76	10858.0
77	7592.0
78	4326.0
79	3071.0
80	1816.0
81	1816.0
82	1306.5
83	797.0
84	545.0
85	293.0
86	229.5
87	166.0
88	97.5
89	29.0
90	17.0
91	5.0
92	3.0
93	1.0
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1.4968197E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.745118008182914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.0968889068609	32.98080691067206
2	14.51453277383721	13.279380291456434
3	4.669337918241549	6.407982422701286
4	2.222797450755694	4.067285267724295
5	1.2723813019093027	2.9102616403623234
6	0.8668612379016026	2.3792801774717
7	0.6567568400550893	2.1030393405700964
8	0.5205642095514647	1.9050616957414568
9	0.43952294489969856	1.809542608356671
>10	2.6175301984705763	20.597272635609308
>50	0.08903185684265014	2.7182931528935006
>100	0.030957348132592568	2.574230613392856
>500	0.0017256758039672613	0.5356104835913076
>1k	9.68456780851303E-4	0.8757324413960386
>5k	6.350220303181521E-5	0.1749340277579221
>10k+	7.937775378976903E-5	4.681286290302728
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	540678	3.612178540942506	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGC	34904	0.23318773797538878	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC	30418	0.2032175284705299	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGG	27586	0.18429741404392258	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.001910717770483646	0.0	0.0	0.0	6.680831365327434E-6
2	0.003260245706279788	0.0	0.0	0.0	2.6723325461309736E-5
3	0.0032736073690104424	0.0	0.0	0.0	3.340415682663717E-5
4	0.003293649863106425	0.0	0.0	0.0	3.340415682663717E-5
5	0.00330701152583708	0.0	0.0	0.0	4.00849881919646E-5
6	0.003507436466796903	0.0	0.0	0.0	4.00849881919646E-5
7	0.003520798129527558	0.0	0.0	6.680831365327434E-6	6.0127482287946906E-5
8	0.0035341597922582124	0.0	0.0	1.3361662730654868E-5	8.685080774925664E-5
9	0.003554202286354195	1.3361662730654868E-5	0.0	1.3361662730654868E-5	8.685080774925664E-5
10	0.003574244780450177	1.3361662730654868E-5	0.0	1.3361662730654868E-5	9.353163911458407E-5
11	0.0036076489372768145	1.3361662730654868E-5	0.0	2.00424940959823E-5	1.1357413321056638E-4
12	0.003667776419564761	1.3361662730654868E-5	0.0	2.00424940959823E-5	1.4029745867187612E-4
13	0.0036944997450260707	1.3361662730654868E-5	0.0	2.6723325461309736E-5	1.4697829003720354E-4
14	0.0037613080586793453	1.3361662730654868E-5	0.0	2.6723325461309736E-5	1.603399527678584E-4
15	0.0037613080586793453	1.3361662730654868E-5	0.0	4.00849881919646E-5	1.6702078413318586E-4
16	0.0038281163723326195	1.3361662730654868E-5	0.0	4.00849881919646E-5	1.8038244686384073E-4
17	0.003868201360524584	1.3361662730654868E-5	0.0	4.00849881919646E-5	1.8706327822916815E-4
18	0.003928328842812531	1.3361662730654868E-5	0.0	4.00849881919646E-5	2.0710577232515044E-4
19	0.003955052168273841	1.3361662730654868E-5	0.0	4.00849881919646E-5	2.1378660369047789E-4
20	0.003981775493735151	1.3361662730654868E-5	0.0	4.00849881919646E-5	2.538715918824425E-4
21	0.004021860481927115	1.3361662730654868E-5	0.0	4.00849881919646E-5	2.6055242324776994E-4
22	0.004048583807388425	1.3361662730654868E-5	0.0	4.00849881919646E-5	2.8059491734375223E-4
23	0.004142115446503009	1.3361662730654868E-5	0.0	4.00849881919646E-5	3.006374114397345E-4
24	0.004182200434694973	1.3361662730654868E-5	0.0	4.00849881919646E-5	3.07318242805062E-4
25	0.004249008748348248	1.3361662730654868E-5	0.0	4.00849881919646E-5	3.07318242805062E-4
26	0.004469476183404053	1.3361662730654868E-5	0.0	4.00849881919646E-5	3.1399907417038937E-4
27	0.004549646159787982	2.00424940959823E-5	0.0	4.00849881919646E-5	3.340415682663717E-4
28	0.0046298161361719116	2.00424940959823E-5	0.0	4.676581955729204E-5	3.340415682663717E-4
29	0.004703305281190513	2.00424940959823E-5	0.0	5.344665092261947E-5	3.407223996316991E-4
30	0.004836921908497062	2.00424940959823E-5	0.0	5.344665092261947E-5	3.6076489372768145E-4
31	0.005090793500379505	2.00424940959823E-5	0.0	5.344665092261947E-5	3.874882191889912E-4
32	0.005431515900011204	2.00424940959823E-5	0.0	6.0127482287946906E-5	3.941690505543186E-4
33	0.005651983335067009	2.00424940959823E-5	0.0	6.680831365327433E-5	4.142115446503009E-4
34	0.005839046613296177	2.6723325461309736E-5	0.0	7.348914501860177E-5	4.342540387462832E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	224515	0.0	38.850857	1
AGCAGTG	229180	0.0	38.043457	2
GTGGTAT	228900	0.0	37.862823	6
TCAACGC	229405	0.0	37.853577	12
AGTGGTA	229160	0.0	37.841682	5
CAACGCA	229705	0.0	37.81633	13
ATCAACG	230795	0.0	37.746918	11
GTATCAA	229780	0.0	37.730873	9
GGTATCA	229845	0.0	37.722813	8
AACGCAG	230455	0.0	37.719296	14
ACGCAGA	230615	0.0	37.686188	15
TGGTATC	229900	0.0	37.668552	7
TATCAAC	230185	0.0	37.645374	10
GCAGTGG	231685	0.0	37.614864	3
CGCAGAG	232265	0.0	37.462383	16
CAGTGGT	231495	0.0	37.45653	4
AGAGTAC	230195	0.0	37.437824	19
CAGAGTA	231700	0.0	37.205006	18
GCAGAGT	233600	0.0	37.141266	17
GTACATG	164540	0.0	37.00377	22
>>END_MODULE