FastQCFastQC Report
Thu 26 May 2016
SRR805194_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805194_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24845205
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT10621514.275074405705246No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGC1063960.42823554887150256No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGG838900.3376506653899615No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC772740.3110217846864214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT4599400.039.2820781
AGCAGTG4706900.038.361982
AGTGGTA4728800.038.256645
GTGGTAT4732900.038.2277266
TCAACGC4750800.038.20367412
CAACGCA4752300.038.18487513
TGGTATC4749700.038.1274647
ATCAACG4763500.038.1139911
GGTATCA4759600.038.1061448
GTATCAA4763350.038.091689
AACGCAG4766900.038.08009314
ACGCAGA4766600.038.05941415
TATCAAC4765450.038.03502310
CGCAGAG4780700.037.94716616
CAGTGGT4752700.037.9451684
AGAGTAC4726600.037.93424619
GCAGTGG4765450.037.8952643
CAGAGTA4762200.037.781718
GCAGAGT4802250.037.65984717
ACATGGG3460500.037.6136424