##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805194_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24845205
Sequences flagged as poor quality	0
Sequence length	46
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.204198435875256	37.0	35.0	37.0	35.0	37.0
2	36.18751062830836	37.0	36.0	37.0	35.0	37.0
3	37.949797556510404	39.0	38.0	39.0	35.0	39.0
4	37.84423622183838	39.0	38.0	39.0	35.0	39.0
5	37.85899279156683	39.0	38.0	39.0	35.0	39.0
6	37.776991455695374	39.0	38.0	39.0	35.0	39.0
7	37.734559082929685	39.0	38.0	39.0	35.0	39.0
8	38.76066456284019	40.0	39.0	40.0	36.0	40.0
9	38.80414558865584	40.0	39.0	40.0	36.0	40.0
10	38.50898070673999	40.0	38.0	40.0	36.0	40.0
11	38.707091408583665	40.0	39.0	40.0	36.0	40.0
12	38.76829492853852	40.0	39.0	40.0	36.0	40.0
13	38.83232032096334	40.0	39.0	40.0	36.0	40.0
14	38.82764054472483	40.0	39.0	40.0	36.0	40.0
15	38.8162655933006	40.0	39.0	40.0	36.0	40.0
16	38.760957295381544	40.0	39.0	40.0	36.0	40.0
17	38.75778936016024	40.0	39.0	40.0	36.0	40.0
18	38.742548833869556	40.0	39.0	40.0	36.0	40.0
19	38.70170719058265	40.0	39.0	40.0	36.0	40.0
20	38.629879970803216	40.0	39.0	40.0	36.0	40.0
21	38.5614383540003	40.0	39.0	40.0	36.0	40.0
22	38.60133567020276	40.0	39.0	40.0	36.0	40.0
23	38.20760722239965	40.0	38.0	40.0	35.0	40.0
24	38.32232541450151	40.0	38.0	40.0	35.0	40.0
25	38.451482610024755	40.0	39.0	40.0	35.0	40.0
26	38.458234335357666	40.0	39.0	40.0	35.0	40.0
27	38.467393044251395	40.0	39.0	40.0	35.0	40.0
28	38.41378909934533	40.0	39.0	40.0	35.0	40.0
29	38.35207264339336	40.0	39.0	40.0	35.0	40.0
30	38.33259810092128	40.0	39.0	40.0	35.0	40.0
31	38.233805557249376	40.0	38.0	40.0	35.0	40.0
32	38.194930369864124	40.0	38.0	40.0	35.0	40.0
33	38.035742470227156	40.0	38.0	40.0	34.0	40.0
34	38.0618491173649	40.0	38.0	40.0	34.0	40.0
35	37.99911886418325	40.0	38.0	40.0	34.0	40.0
36	37.932611101417756	40.0	38.0	40.0	34.0	40.0
37	37.834951814645926	40.0	37.0	40.0	34.0	40.0
38	37.72896242957142	40.0	37.0	40.0	34.0	40.0
39	37.720743217856324	40.0	37.0	40.0	33.0	40.0
40	37.63883232197118	40.0	37.0	40.0	33.0	40.0
41	37.56519545723209	40.0	37.0	40.0	33.0	40.0
42	37.42608885698468	40.0	37.0	40.0	33.0	40.0
43	37.30280458543208	40.0	36.0	40.0	33.0	40.0
44	37.35090509416203	40.0	36.0	40.0	33.0	40.0
45	37.26326399802296	40.0	36.0	40.0	33.0	40.0
46	37.10719875324031	40.0	36.0	40.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	5.0
13	26.0
14	80.0
15	222.0
16	549.0
17	1195.0
18	2328.0
19	3938.0
20	6206.0
21	10519.0
22	16906.0
23	26836.0
24	39865.0
25	57087.0
26	80017.0
27	110476.0
28	145033.0
29	186223.0
30	236190.0
31	295040.0
32	365675.0
33	452276.0
34	577768.0
35	770956.0
36	1095716.0
37	1865140.0
38	4290013.0
39	1.4208918E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.780161805869582	43.36105900514808	17.945104498030908	13.913674690951433
2	23.45219127795484	40.52623836269413	19.552549475844533	16.469020883506495
3	39.10585161201125	21.1106811153299	19.959948006063946	19.823519266594904
4	21.17809050076262	23.143809841778324	19.109731636345927	36.56836802111313
5	20.000800959380292	40.559202469852835	19.04635522226522	20.393641348501653
6	38.24067460904428	20.938482898410378	21.789995292854293	19.03084719969105
7	19.304654560105263	21.297151703920335	39.007538879232435	20.390654856741975
8	37.875630327864066	21.273340268273095	21.15244772582879	19.698581678034053
9	38.07671540645368	21.81628608015108	20.717571056467435	19.389427456927805
10	19.22327869703631	22.1225222331633	39.87478469185503	18.77941437794536
11	19.1896867021222	40.24098010058682	21.568680958760453	19.00065223853053
12	19.122084120457046	21.421884021484225	39.99809621212624	19.457935645932483
13	19.172733732726293	21.78260553696377	21.435327259324282	37.60933347098565
14	19.187404571626598	40.04514754456645	21.467184513068013	19.30026337073894
15	19.439533704793337	39.554960403828424	21.39178163351842	19.613724257859815
16	19.641653188210764	21.315453022021753	21.242638167002443	37.800255622765036
17	37.719809516564666	21.372570683155963	21.393705545999723	19.513914254279648
18	19.415295627466143	21.32974551830021	21.391093371940382	37.863865482293264
19	19.791287695150835	39.78900958957674	21.174693467009025	19.245009248263397
20	37.9926871201103	21.416744196717232	21.31705091586083	19.273517767311642
21	19.706237078744167	39.437460870216206	21.380419280098515	19.475882770941112
22	38.07718632227024	21.30328568430005	21.36667819806679	19.252849795362927
23	19.58684180710121	21.44692708311322	39.481674632992565	19.484556476793006
24	19.530867223675553	39.47856336866611	21.171372906764102	19.819196500894236
25	19.711236031258345	21.394776175121116	21.267455833026936	37.6265319605936
26	19.842669843134722	34.67367646996674	26.154173410925768	19.32948027597277
27	20.039649501785153	21.02950247341489	39.37910353325722	19.551744491542735
28	33.62533333896822	21.239212958798287	25.828557260847717	19.306896441385774
29	33.450587346733506	21.231392536306302	25.926362853516405	19.39165726344379
30	33.15861551554918	21.183407422076012	26.023922925973043	19.634054136401772
31	28.45748706843031	23.319743185858197	26.994202704304517	21.228567041406983
32	24.716427173774576	25.426777521054866	28.034282671445055	21.822512633725502
33	24.24877959348695	24.200464435692922	28.726657719266154	22.824098251553973
34	23.654459683468097	23.936288712449745	29.261919955983455	23.1473316480987
35	22.966572423129534	24.14765344057334	29.660511957941182	23.225262178355944
36	23.036030493610337	24.21689014037115	29.664617377880358	23.082461988138153
37	24.195984698053408	24.027094966614282	29.231873916918776	22.545046418413534
38	24.101761285527733	24.098931765706904	29.34564637321366	22.4536605755517
39	24.083520341248946	24.000204466012658	29.243200046045104	22.673075146693293
40	23.6284305160694	24.612620423135972	28.74542592826262	23.01352313253201
41	23.643020856539522	25.207129504465755	28.659312732577575	22.490536906417155
42	23.861352723795196	24.99236371766705	28.724749906470887	22.42153365206687
43	23.9235216614232	24.626510427263533	29.21071490454597	22.2392530067673
44	23.784738342871393	24.35651466751834	29.039200924282977	22.81954606532729
45	23.997109301372234	23.750272939989827	28.859673325295564	23.392944433342368
46	23.965332546058686	23.939391926933183	28.51404526547477	23.581230261533364
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7408.0
1	4833.0
2	2258.0
3	2105.0
4	1952.0
5	2164.5
6	2377.0
7	2377.0
8	3007.0
9	3637.0
10	4980.5
11	6324.0
12	9552.0
13	12780.0
14	17355.5
15	21931.0
16	30497.5
17	39064.0
18	54556.5
19	70049.0
20	70049.0
21	92638.0
22	115227.0
23	150562.5
24	185898.0
25	769669.5
26	1353441.0
27	865552.0
28	377663.0
29	402678.5
30	427694.0
31	427694.0
32	483556.5
33	539419.0
34	607829.5
35	676240.0
36	760657.5
37	845075.0
38	937412.5
39	1029750.0
40	1141824.5
41	1253899.0
42	1391710.0
43	1529521.0
44	1529521.0
45	1705219.0
46	1880917.0
47	1983147.0
48	2085377.0
49	2161581.5
50	2237786.0
51	2210973.0
52	2184160.0
53	2098737.0
54	2013314.0
55	1961131.0
56	1908948.0
57	1908948.0
58	1623009.0
59	1337070.0
60	1148181.5
61	959293.0
62	822969.0
63	686645.0
64	569948.5
65	453252.0
66	371466.5
67	289681.0
68	224350.5
69	159020.0
70	159020.0
71	118198.0
72	77376.0
73	57065.5
74	36755.0
75	27887.0
76	19019.0
77	13662.5
78	8306.0
79	5951.5
80	3597.0
81	3597.0
82	2688.5
83	1780.0
84	1309.0
85	838.0
86	606.5
87	375.0
88	217.5
89	60.0
90	38.0
91	16.0
92	11.5
93	7.0
94	7.0
95	6.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	2.4845205E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.96051345482431
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.55453713636135	26.07731919127385
2	14.322034591207744	10.58699504417586
3	5.083585340815705	5.636757731638994
4	2.4768782002290823	3.6618676018211205
5	1.4198327840369018	2.623887435899828
6	0.943018260513487	2.091266346351225
7	0.6563265095905944	1.6980715351936726
8	0.49272023242062385	1.4568954223877302
9	0.3977429462406321	1.3230705164479508
>10	3.3485563113870067	26.061587644778843
>50	0.2241025942947606	5.557921441343182
>100	0.07451177152272516	4.819667285246826
>500	0.004231965515672208	1.058053755601411
>1k	0.0016380692989825015	1.1090774545541235
>5k	1.7433019383736035E-4	0.4589344809168425
>10k+	1.0895637114801752E-4	5.778627112368478
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	1062151	4.275074405705246	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGC	106396	0.42823554887150256	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGG	83890	0.3376506653899615	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC	77274	0.3110217846864214	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.210839878358822E-4	4.0249215089994225E-6	0.0	0.0	8.049843017998845E-6
2	8.412085953808793E-4	4.0249215089994225E-6	0.0	4.0249215089994225E-6	1.609968603599769E-5
3	8.57308281416877E-4	4.0249215089994225E-6	0.0	4.0249215089994225E-6	1.609968603599769E-5
4	8.653581244348759E-4	4.0249215089994225E-6	0.0	4.0249215089994225E-6	4.0249215089994224E-5
5	8.734079674528747E-4	4.0249215089994225E-6	0.0	8.049843017998845E-6	6.842366565299019E-5
6	9.015824180158707E-4	4.0249215089994225E-6	0.0	8.049843017998845E-6	7.647350867098904E-5
7	9.13657182542869E-4	4.0249215089994225E-6	0.0	1.2074764526998268E-5	8.452335168898787E-5
8	9.13657182542869E-4	4.0249215089994225E-6	0.0	1.2074764526998268E-5	1.0867288074298441E-4
9	9.257319470698672E-4	4.0249215089994225E-6	0.0	1.2074764526998268E-5	1.1672272376098326E-4
10	9.418316331058649E-4	4.0249215089994225E-6	0.0	1.2074764526998268E-5	1.247725667789821E-4
11	9.659811621598615E-4	4.0249215089994225E-6	0.0	1.2074764526998268E-5	1.5294701734197807E-4
12	9.861057697048586E-4	4.0249215089994225E-6	0.0	1.2074764526998268E-5	1.6502178186897633E-4
13	0.0010142802202678545	8.049843017998845E-6	0.0	1.2074764526998268E-5	1.770965463959746E-4
14	0.0010625792783758477	8.049843017998845E-6	0.0	1.2074764526998268E-5	1.8917131092297286E-4
15	0.001086728807429844	1.2074764526998268E-5	0.0	2.0124607544997112E-5	2.0124607544997114E-4
16	0.0011269780225198383	1.609968603599769E-5	0.0	2.0124607544997112E-5	2.3344544752196652E-4
17	0.0011511275515738348	1.609968603599769E-5	0.0	2.0124607544997112E-5	2.495451335579642E-4
18	0.0011833269236458303	1.609968603599769E-5	0.0	2.0124607544997112E-5	2.7771958412096015E-4
19	0.001227601060244824	1.609968603599769E-5	0.0	2.0124607544997112E-5	3.340684852469521E-4
20	0.0012598004323168192	1.609968603599769E-5	0.0	2.0124607544997112E-5	3.380934067559515E-4
21	0.0012919998043888147	1.609968603599769E-5	0.0	2.4149529053996537E-5	3.421183282649509E-4
22	0.0013563985485328055	1.609968603599769E-5	0.0	2.4149529053996537E-5	3.582180143009486E-4
23	0.0014207972926767961	1.609968603599769E-5	0.0	2.4149529053996537E-5	3.662678573189475E-4
24	0.0015093455658747835	2.4149529053996537E-5	0.0	2.4149529053996537E-5	3.702927788279469E-4
25	0.0015576446239827766	2.4149529053996537E-5	0.0	2.817445056299596E-5	3.823675433549451E-4
26	0.001658267661707762	2.4149529053996537E-5	0.0	2.817445056299596E-5	4.145669154269405E-4
27	0.0017387660918877505	2.4149529053996537E-5	0.0	2.817445056299596E-5	4.226167584449394E-4
28	0.0018232894435767383	2.4149529053996537E-5	0.0	3.219937207199538E-5	4.5481613051693475E-4
29	0.0019480620103557206	2.4149529053996537E-5	0.0	3.6224293580994805E-5	4.5884105202593416E-4
30	0.0021291834782606947	2.817445056299596E-5	0.0	3.6224293580994805E-5	4.7896565957093127E-4
31	0.0022901803386206716	2.817445056299596E-5	0.0	4.0249215089994224E-5	4.8299058107993074E-4
32	0.00243910243445365	2.817445056299596E-5	0.0	4.0249215089994224E-5	5.232397961699249E-4
33	0.002696697411029613	2.817445056299596E-5	0.0	4.0249215089994224E-5	5.473893252239215E-4
34	0.002901968407988584	2.817445056299596E-5	0.0	4.0249215089994224E-5	5.51414246732921E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	459940	0.0	39.282078	1
AGCAGTG	470690	0.0	38.36198	2
AGTGGTA	472880	0.0	38.25664	5
GTGGTAT	473290	0.0	38.227726	6
TCAACGC	475080	0.0	38.203674	12
CAACGCA	475230	0.0	38.184875	13
TGGTATC	474970	0.0	38.127464	7
ATCAACG	476350	0.0	38.11399	11
GGTATCA	475960	0.0	38.106144	8
GTATCAA	476335	0.0	38.09168	9
AACGCAG	476690	0.0	38.080093	14
ACGCAGA	476660	0.0	38.059414	15
TATCAAC	476545	0.0	38.035023	10
CGCAGAG	478070	0.0	37.947166	16
CAGTGGT	475270	0.0	37.945168	4
AGAGTAC	472660	0.0	37.934246	19
GCAGTGG	476545	0.0	37.895264	3
CAGAGTA	476220	0.0	37.7817	18
GCAGAGT	480225	0.0	37.659847	17
ACATGGG	346050	0.0	37.61364	24
>>END_MODULE