FastQCFastQC Report
Thu 26 May 2016
SRR805193_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805193_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22860800
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT12674825.544346654535275No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGC1038790.4543979213325867No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGG868480.37989921612541994No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC674780.29516902295632697No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGCTGGTGAGATG299710.131102148656215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT5163650.039.3210221
AGCAGTG5236700.038.7614332
GTGGTAT5265500.038.6227346
AGTGGTA5267100.038.611765
TCAACGC5280700.038.60851712
CAACGCA5285900.038.5656213
ATCAACG5292000.038.54308311
GTATCAA5293100.038.5297859
TGGTATC5284200.038.52397
GGTATCA5292000.038.5207868
AACGCAG5298900.038.482714
ACGCAGA5297550.038.4717515
TATCAAC5295350.038.4714910
GCAGTGG5290950.038.3632473
CGCAGAG5314700.038.34496716
AGAGTAC5250350.038.33763519
CAGTGGT5289250.038.311674
CAGAGTA5288250.038.19902818
ACATGGG3723050.038.07953324
GCAGAGT5333500.038.0748117