##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805193_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22860800
Sequences flagged as poor quality	0
Sequence length	46
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.25724143512038	37.0	36.0	37.0	35.0	37.0
2	36.24567547942329	37.0	37.0	37.0	35.0	37.0
3	38.02170361492161	39.0	39.0	39.0	35.0	39.0
4	37.90064201602743	39.0	38.0	39.0	35.0	39.0
5	37.889741128919376	39.0	38.0	39.0	35.0	39.0
6	37.82939149111142	39.0	38.0	39.0	35.0	39.0
7	37.76600044617861	39.0	38.0	39.0	35.0	39.0
8	38.78825990341545	40.0	39.0	40.0	36.0	40.0
9	38.84427679696248	40.0	39.0	40.0	36.0	40.0
10	38.482378263227886	40.0	38.0	40.0	36.0	40.0
11	38.72004536149216	40.0	39.0	40.0	36.0	40.0
12	38.80457210596305	40.0	39.0	40.0	36.0	40.0
13	38.87764754514278	40.0	39.0	40.0	36.0	40.0
14	38.88627038423852	40.0	39.0	40.0	36.0	40.0
15	38.8710819393897	40.0	39.0	40.0	36.0	40.0
16	38.80457599909015	40.0	39.0	40.0	36.0	40.0
17	38.81420199643057	40.0	39.0	40.0	36.0	40.0
18	38.79297522396416	40.0	39.0	40.0	36.0	40.0
19	38.74305916678331	40.0	39.0	40.0	36.0	40.0
20	38.69378993736002	40.0	39.0	40.0	36.0	40.0
21	38.58823588850784	40.0	39.0	40.0	36.0	40.0
22	38.64250524916014	40.0	39.0	40.0	36.0	40.0
23	38.1442225993841	40.0	38.0	40.0	35.0	40.0
24	38.30975346444569	40.0	38.0	40.0	35.0	40.0
25	38.48548738451848	40.0	39.0	40.0	35.0	40.0
26	38.49371771766517	40.0	39.0	40.0	35.0	40.0
27	38.527181944638855	40.0	39.0	40.0	35.0	40.0
28	38.46924783909574	40.0	39.0	40.0	35.0	40.0
29	38.38944385148376	40.0	39.0	40.0	35.0	40.0
30	38.37998455872061	40.0	39.0	40.0	35.0	40.0
31	38.263719467385215	40.0	38.0	40.0	35.0	40.0
32	38.22534535099384	40.0	38.0	40.0	35.0	40.0
33	38.0484024181131	40.0	38.0	40.0	34.0	40.0
34	38.072104869470884	40.0	38.0	40.0	34.0	40.0
35	38.01625358692609	40.0	38.0	40.0	34.0	40.0
36	37.93780239536674	40.0	38.0	40.0	34.0	40.0
37	37.821066585596306	40.0	37.0	40.0	34.0	40.0
38	37.6810507506299	40.0	37.0	40.0	33.0	40.0
39	37.67725451427771	40.0	37.0	40.0	33.0	40.0
40	37.59168620520717	40.0	37.0	40.0	33.0	40.0
41	37.50230630599104	40.0	36.0	40.0	33.0	40.0
42	37.37488994260918	40.0	36.0	40.0	33.0	40.0
43	37.25689337206047	40.0	36.0	40.0	33.0	40.0
44	37.29475814494681	40.0	36.0	40.0	33.0	40.0
45	37.1975156162514	40.0	35.0	40.0	33.0	40.0
46	37.0039415506019	39.0	35.0	40.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	8.0
13	7.0
14	51.0
15	146.0
16	401.0
17	878.0
18	1644.0
19	2695.0
20	4672.0
21	7913.0
22	13264.0
23	21196.0
24	32646.0
25	47986.0
26	68722.0
27	96729.0
28	128104.0
29	167114.0
30	213278.0
31	268501.0
32	331304.0
33	412351.0
34	525656.0
35	706516.0
36	1010493.0
37	1758070.0
38	4160839.0
39	1.2879614E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.902999895016796	42.65341545352744	26.82141044932811	16.62217420212766
2	21.852043673012318	47.38355613101904	12.46413948768197	18.300260708286675
3	44.46588483342665	21.71740708986562	15.44111754619261	18.37559053051512
4	20.989331082026876	19.28603985862262	16.907536044232923	42.81709301511758
5	19.25629461786114	42.04529587765957	19.40492896136618	19.293480543113102
6	42.33663301371781	19.993827862541995	18.0106601693729	19.6588789543673
7	19.983570128779395	19.35262545492721	41.62656162513998	19.037242791153417
8	41.63128586926092	20.965311800111984	19.060347844344903	18.343054486282195
9	41.2999763787794	20.677115411534157	19.294232922732363	18.72867528695409
10	18.682941979283317	20.641504234322507	42.048112926931694	18.627440859462485
11	18.659215775475925	42.646757768757	19.853977113661813	18.840049342105264
12	18.526149566069428	20.560750279955204	42.06817346724524	18.844926686730123
13	18.45643634518477	20.626176686730126	20.043025615901456	40.874361352183655
14	18.82386443169093	42.74822840845465	19.787080942049272	18.640826217805152
15	18.751579122340427	42.52992896136618	19.81415785974244	18.904334056550955
16	18.73653590425532	20.209730193169094	20.077823173292273	40.975910729283314
17	41.01734409994401	20.15695426231803	20.00450115481523	18.82120048292273
18	18.787185925251958	20.293511163213886	19.977901035834268	40.94140187569989
19	18.828461821108622	42.529596514557674	20.061375804871222	18.580565859462485
20	41.239689774636055	20.214279465285554	20.043834861422173	18.502195898656215
21	18.869890817469205	42.356697928331464	20.090342420212767	18.683068833986564
22	40.88163143896976	20.135161499160134	20.316620590705487	18.666586471164614
23	18.875166223404253	20.293218085106382	42.04370800671893	18.787907684770435
24	18.84341755319149	42.21109060050392	19.99150073488242	18.953991111422173
25	18.771687779955208	20.28332779255319	20.217958251679732	40.72702617581187
26	19.126202932530795	35.94394334406495	26.17956064529675	18.750293078107504
27	19.312889312709967	20.121220604703247	41.86011425671893	18.705775825867864
28	35.07896923992161	20.05263595324748	26.23269964305711	18.635695163773796
29	35.15505144176932	20.118683510638295	26.008862332026876	18.71740271556551
30	34.841562849944005	20.18909224524076	26.065203317469205	18.904141587346025
31	28.973373635218362	22.46932740761478	27.60963308370661	20.94766587346025
32	24.94734217525196	25.03018704507279	28.494938934770435	21.527531844904814
33	24.35815894456887	23.720784049552073	29.369344029955208	22.551712975923852
34	23.323877554591267	23.297854843225082	30.20204892217245	23.1762186800112
35	22.877545842665175	23.51383153695409	30.439258468645015	23.16936415173572
36	22.80263595324748	23.806664683650617	30.58288861282195	22.807810750279955
37	23.99797907334826	23.35829892217245	30.226776840705487	22.416945163773796
38	24.163721304591267	23.522619505879057	30.019644981802912	22.294014207726764
39	23.99428278975364	23.507453807390817	30.132200097984324	22.36606330487122
40	23.353942119260918	24.057530795072786	29.74531949888018	22.843207586786114
41	23.69284102043673	24.62914683650616	29.30963483342665	22.368377309630457
42	23.832543043113102	24.60322035974244	29.48765135078387	22.076585246360583
43	23.66584721444569	24.021950237961924	30.328881753919372	21.983320793673013
44	23.754549272116463	23.751124195128778	29.790081711926092	22.704244820828666
45	23.9265117581187	23.28191489361702	29.667172627379617	23.12440072088466
46	23.66035309350504	23.36400738381859	29.48626907194849	23.489370450727883
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3679.0
1	2264.0
2	849.0
3	774.5
4	700.0
5	829.0
6	958.0
7	958.0
8	1335.5
9	1713.0
10	2542.5
11	3372.0
12	5653.5
13	7935.0
14	10827.0
15	13719.0
16	20007.5
17	26296.0
18	38519.5
19	50743.0
20	50743.0
21	67446.0
22	84149.0
23	116372.0
24	148595.0
25	825316.0
26	1502037.0
27	909740.5
28	317444.0
29	330395.0
30	343346.0
31	343346.0
32	390538.5
33	437731.0
34	496314.0
35	554897.0
36	632776.0
37	710655.0
38	798244.5
39	885834.0
40	989529.0
41	1093224.0
42	1224815.5
43	1356407.0
44	1356407.0
45	1526301.5
46	1696196.0
47	1798012.5
48	1899829.0
49	1990998.5
50	2082168.0
51	2073743.0
52	2065318.0
53	1986491.5
54	1907665.0
55	1875005.5
56	1842346.0
57	1842346.0
58	1550104.0
59	1257862.0
60	1089331.0
61	920800.0
62	786367.5
63	651935.0
64	539852.0
65	427769.0
66	350135.0
67	272501.0
68	210457.5
69	148414.0
70	148414.0
71	111247.0
72	74080.0
73	55060.5
74	36041.0
75	27530.0
76	19019.0
77	13666.5
78	8314.0
79	5997.0
80	3680.0
81	3680.0
82	2594.0
83	1508.0
84	1055.0
85	602.0
86	499.0
87	396.0
88	220.5
89	45.0
90	31.5
91	18.0
92	12.5
93	7.0
94	7.0
95	5.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	2.28608E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.53703561550361
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.302696052014	24.628123778508424
2	14.796037005138816	10.516145880398547
3	5.395544479697032	5.75224969020082
4	2.627528492890112	3.7349829453034578
5	1.5608781949931774	2.7734492003467768
6	1.0208362448882977	2.176649639531543
7	0.7067678238167204	1.7581503328806738
8	0.5103174250948485	1.450813480864618
9	0.3963987723072729	1.2678153560482914
>10	3.3597101948519343	25.933353000376503
>50	0.24390578030162238	5.743346286844333
>100	0.07341283721745405	4.507743989134019
>500	0.003866892984986707	0.9368341240788409
>1k	0.0018176610448525285	1.3049793541361843
>5k	1.8400614707017438E-4	0.49757681789144736
>10k+	9.81366117706831E-5	7.017786123455669
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	1267482	5.544346654535275	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGC	103879	0.4543979213325867	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGG	86848	0.37989921612541994	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC	67478	0.29516902295632697	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGCTGGTGAGATG	29971	0.131102148656215	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.001272921332586786	4.374300111982083E-6	0.0	0.0	4.374300111982083E-6
2	0.0015791223404255318	4.374300111982083E-6	0.0	4.374300111982083E-6	3.499440089585666E-5
3	0.0018240831466965286	8.748600223964166E-6	4.374300111982083E-6	4.374300111982083E-6	3.499440089585666E-5
4	0.002379619260918253	1.3122900335946248E-5	4.374300111982083E-6	1.3122900335946248E-5	5.249160134378499E-5
5	0.0024014907614781636	1.3122900335946248E-5	4.374300111982083E-6	1.3122900335946248E-5	9.623460246360582E-5
6	0.0024758538633818587	2.1871500559910414E-5	4.374300111982083E-6	1.749720044792833E-5	1.0935750279955208E-4
7	0.0024846024636058233	2.1871500559910414E-5	4.374300111982083E-6	1.749720044792833E-5	1.312290033594625E-4
8	0.0025064739641657333	2.1871500559910414E-5	4.374300111982083E-6	1.749720044792833E-5	1.3560330347144457E-4
9	0.002532719764837626	2.6245800671892496E-5	4.374300111982083E-6	1.749720044792833E-5	1.4872620380739082E-4
10	0.002532719764837626	2.6245800671892496E-5	4.374300111982083E-6	1.749720044792833E-5	1.6622340425531914E-4
11	0.00254146836506159	2.6245800671892496E-5	4.374300111982083E-6	1.749720044792833E-5	2.012178051511758E-4
12	0.0025502169652855543	2.6245800671892496E-5	4.374300111982083E-6	2.1871500559910414E-5	2.3621220604703248E-4
13	0.0025633398656215007	3.499440089585666E-5	4.374300111982083E-6	2.6245800671892496E-5	2.4058650615901457E-4
14	0.002585211366181411	3.499440089585666E-5	4.374300111982083E-6	2.6245800671892496E-5	2.537094064949608E-4
15	0.0026027085666293394	3.499440089585666E-5	4.374300111982083E-6	3.062010078387458E-5	2.6683230683090703E-4
16	0.002650825867861142	3.499440089585666E-5	4.374300111982083E-6	3.062010078387458E-5	2.8870380739081747E-4
17	0.002733937569988802	3.499440089585666E-5	4.374300111982083E-6	3.062010078387458E-5	3.105753079507279E-4
18	0.0027820548712206047	3.499440089585666E-5	4.374300111982083E-6	3.062010078387458E-5	3.105753079507279E-4
19	0.002817049272116461	3.499440089585666E-5	4.374300111982083E-6	3.062010078387458E-5	3.236982082866741E-4
20	0.0028476693729003358	3.499440089585666E-5	4.374300111982083E-6	3.062010078387458E-5	3.586926091825308E-4
21	0.0029745240761478164	3.499440089585666E-5	4.374300111982083E-6	3.062010078387458E-5	3.849384098544233E-4
22	0.0030401385778275475	3.499440089585666E-5	4.374300111982083E-6	3.062010078387458E-5	4.1118421052631577E-4
23	0.003110127379619261	3.499440089585666E-5	4.374300111982083E-6	3.062010078387458E-5	4.374300111982083E-4
24	0.003180116181410974	3.9368701007838745E-5	4.374300111982083E-6	3.062010078387458E-5	4.593015117581187E-4
25	0.0032544792833146697	4.374300111982083E-5	4.374300111982083E-6	3.062010078387458E-5	4.593015117581187E-4
26	0.003385708286674132	4.811730123180291E-5	4.374300111982083E-6	3.062010078387458E-5	4.593015117581187E-4
27	0.003490691489361702	4.811730123180291E-5	4.374300111982083E-6	3.062010078387458E-5	4.899216125419933E-4
28	0.0036656634938409855	5.249160134378499E-5	4.374300111982083E-6	3.499440089585666E-5	5.161674132138857E-4
29	0.0038100153975363944	5.249160134378499E-5	4.374300111982083E-6	3.499440089585666E-5	5.2491601343785E-4
30	0.00401123320268757	5.249160134378499E-5	4.374300111982083E-6	3.499440089585666E-5	5.511618141097424E-4
31	0.004208076707726763	5.686590145576708E-5	4.374300111982083E-6	3.499440089585666E-5	5.555361142217246E-4
32	0.0045099034154535275	6.124020156774916E-5	4.374300111982083E-6	3.499440089585666E-5	5.555361142217246E-4
33	0.004737367021276596	6.124020156774916E-5	4.374300111982083E-6	3.499440089585666E-5	5.686590145576707E-4
34	0.004916713325867861	6.124020156774916E-5	4.374300111982083E-6	3.499440089585666E-5	6.036534154535275E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	516365	0.0	39.321022	1
AGCAGTG	523670	0.0	38.761433	2
GTGGTAT	526550	0.0	38.622734	6
AGTGGTA	526710	0.0	38.61176	5
TCAACGC	528070	0.0	38.608517	12
CAACGCA	528590	0.0	38.56562	13
ATCAACG	529200	0.0	38.543083	11
GTATCAA	529310	0.0	38.529785	9
TGGTATC	528420	0.0	38.5239	7
GGTATCA	529200	0.0	38.520786	8
AACGCAG	529890	0.0	38.4827	14
ACGCAGA	529755	0.0	38.47175	15
TATCAAC	529535	0.0	38.47149	10
GCAGTGG	529095	0.0	38.363247	3
CGCAGAG	531470	0.0	38.344967	16
AGAGTAC	525035	0.0	38.337635	19
CAGTGGT	528925	0.0	38.31167	4
CAGAGTA	528825	0.0	38.199028	18
ACATGGG	372305	0.0	38.079533	24
GCAGAGT	533350	0.0	38.07481	17
>>END_MODULE