FastQCFastQC Report
Thu 26 May 2016
SRR805192_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805192_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23964212
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT2540361.0600640655323865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1082000.036.7486151
AGCAGTG1162300.034.2166372
TCAACGC1168850.034.09847312
CAACGCA1174100.033.96814713
AGTGGTA1178650.033.7691425
ATCAACG1182650.033.72595211
GTGGTAT1180600.033.7167556
GGTATCA1183200.033.7052088
AACGCAG1184150.033.703514
GTATCAA1186100.033.6430329
ACGCAGA1186900.033.61698515
TGGTATC1190200.033.4700057
TATCAAC1195100.033.35620510
GCAGTGG1201500.033.1086163
AGAGTAC1192300.033.09737819
CGCAGAG1207150.033.06316
CAGTGGT1204950.032.9474224
CAGAGTA1220750.032.4128618
GCAGAGT1242500.032.0144917
ACATGGG869000.031.75143824
Produced by FastQC (version 0.11.5)