##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805192_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23964212
Sequences flagged as poor quality	0
Sequence length	46
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.24269640078297	37.0	37.0	37.0	35.0	37.0
2	36.18368907769636	37.0	36.0	37.0	35.0	37.0
3	37.920955172654956	39.0	38.0	39.0	35.0	39.0
4	37.82980012027936	39.0	38.0	39.0	35.0	39.0
5	37.85758388383478	39.0	38.0	39.0	35.0	39.0
6	37.74047417040043	39.0	38.0	39.0	35.0	39.0
7	37.75307362495374	39.0	38.0	39.0	35.0	39.0
8	38.74686582642484	40.0	39.0	40.0	36.0	40.0
9	38.767687583468216	40.0	39.0	40.0	36.0	40.0
10	38.710907915520025	40.0	39.0	40.0	36.0	40.0
11	38.76313612982559	40.0	39.0	40.0	36.0	40.0
12	38.74842640350536	40.0	39.0	40.0	36.0	40.0
13	38.78076157897451	40.0	39.0	40.0	36.0	40.0
14	38.762369403175036	40.0	39.0	40.0	36.0	40.0
15	38.75123918115897	40.0	39.0	40.0	36.0	40.0
16	38.711649855209096	40.0	39.0	40.0	36.0	40.0
17	38.71771644316951	40.0	39.0	40.0	36.0	40.0
18	38.69308003951893	40.0	39.0	40.0	36.0	40.0
19	38.66506426332733	40.0	39.0	40.0	36.0	40.0
20	38.56485237236259	40.0	39.0	40.0	36.0	40.0
21	38.55492218980537	40.0	39.0	40.0	36.0	40.0
22	38.541997166441355	40.0	39.0	40.0	36.0	40.0
23	38.43520108234729	40.0	39.0	40.0	35.0	40.0
24	38.40004582666853	40.0	39.0	40.0	35.0	40.0
25	38.43175022821531	40.0	39.0	40.0	35.0	40.0
26	38.450104222079155	40.0	39.0	40.0	35.0	40.0
27	38.412757072921906	40.0	39.0	40.0	35.0	40.0
28	38.381198054832765	40.0	39.0	40.0	35.0	40.0
29	38.36901634821124	40.0	38.0	40.0	35.0	40.0
30	38.349805242918066	40.0	38.0	40.0	35.0	40.0
31	38.30421667943849	40.0	38.0	40.0	35.0	40.0
32	38.289281074629116	40.0	38.0	40.0	35.0	40.0
33	38.221771615106725	40.0	38.0	40.0	35.0	40.0
34	38.23232021983448	40.0	38.0	40.0	35.0	40.0
35	38.15644065408869	40.0	38.0	40.0	34.0	40.0
36	38.11143600298645	40.0	38.0	40.0	34.0	40.0
37	38.06808636144598	40.0	38.0	40.0	34.0	40.0
38	38.02854352148112	40.0	38.0	40.0	34.0	40.0
39	38.01608118806494	40.0	38.0	40.0	34.0	40.0
40	37.9245309213589	40.0	38.0	40.0	34.0	40.0
41	37.84574902775856	40.0	38.0	40.0	34.0	40.0
42	37.714917310863385	40.0	37.0	40.0	33.0	40.0
43	37.586648040002316	40.0	37.0	40.0	33.0	40.0
44	37.63845817254496	40.0	37.0	40.0	33.0	40.0
45	37.55902898038124	40.0	37.0	40.0	33.0	40.0
46	37.365290166853804	40.0	37.0	40.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	9.0
13	21.0
14	75.0
15	260.0
16	613.0
17	1345.0
18	2399.0
19	3976.0
20	6568.0
21	10735.0
22	16833.0
23	25422.0
24	37132.0
25	52557.0
26	72726.0
27	97661.0
28	128895.0
29	167639.0
30	213904.0
31	269636.0
32	339654.0
33	426401.0
34	547393.0
35	733316.0
36	1039622.0
37	1672391.0
38	3608855.0
39	1.4488172E7
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	24.89219340907183	29.163842316200505	24.117237821130942	21.82672645359672
2	24.400364176381014	26.426831810701724	26.489825745156985	22.682978267760276
3	26.35461996413652	24.326295394148577	25.040464505989178	24.278620135725724
4	23.530850920530998	26.157004453140374	24.49015223200329	25.82199239432534
5	25.04943621763987	29.41828423150321	23.5458023823191	21.986477168537817
6	28.114277239743995	24.567784661561166	25.129159264656813	22.188778834038022
7	23.360004493367025	25.123346430084997	29.15773320649976	22.35891587004822
8	27.147602433161584	24.804153793999152	25.182371946968253	22.86587182587101
9	26.739715038408107	24.97194566631275	25.50259945956078	22.785739835718363
10	22.490870970428738	25.246880640181285	29.74992876878238	22.512319620607595
11	22.484845318510786	29.215869897996228	25.6086200539371	22.69066472955589
12	22.399639095164073	25.007594658234538	29.65116482862028	22.941601417981115
13	22.523298491934558	25.21598456898979	25.39139196398363	26.869324975092024
14	22.635903905373564	29.190131517781598	25.41148859808117	22.762475978763668
15	22.75019933891421	28.914387003419932	25.335245740606872	23.000167917058988
16	22.83986220786229	24.94220965830214	25.257625829716414	26.96030230411916
17	26.933595813624084	24.89290697311474	25.333505645835547	22.839991567425628
18	22.794085613998075	24.874412728446902	25.329599821600645	27.001901835954378
19	23.079256684926676	29.023841885558348	25.183135585680848	22.71376584383413
20	27.08443741025159	24.80651982214145	25.348749209863442	22.760293557743523
21	22.933956685076897	28.80259113047406	25.367744201228064	22.89570798322098
22	27.049794084612504	24.820540729651366	25.33233306398725	22.79733212174888
23	22.90790533817678	24.879328391853655	29.34311380653785	22.86965246343172
24	22.830143549055567	28.872199094215993	25.240871679819893	23.05678567690855
25	23.025960544832436	24.832846579724798	25.234074043411063	26.907118832031696
26	23.173442965702357	27.613142464271306	26.46513058722732	22.748283982799016
27	23.24014242571381	24.590405893588322	29.27948559293333	22.889966087764538
28	26.291175357654158	24.723049520676916	26.258468252575966	22.72730686909296
29	26.162149625449814	24.71566767978851	26.323590360492556	22.79859233426912
30	25.99314761528566	24.67494445467266	26.331422873408062	23.000485056633615
31	24.987990425055497	25.27415047071024	26.47872586004497	23.259133244189293
32	24.063954199704128	25.731603442666923	26.7878868706386	23.41655548699035
33	23.918491457177897	25.373002041544286	26.927703694158605	23.780802807119215
34	23.860404840351105	25.440740550951563	27.01031020757119	23.688544401126148
35	23.77517775255869	25.451352208034212	27.060927352837638	23.712542686569456
36	23.69797095769308	25.44004367846521	27.04005038847094	23.821934975370773
37	24.018498918303678	25.46944585534463	26.87864720943046	23.63340801692123
38	24.011071175634736	25.479473307947703	26.913737034207507	23.595718482210057
39	23.984702689159988	25.37855198410029	26.895322074433324	23.741423252306397
40	23.99672895566105	25.535289873082412	26.71302941235873	23.75495175889781
41	23.894801965530934	25.66000918369442	26.80287588842896	23.642312962345684
42	23.967360161894746	25.53705917807771	26.8411371089523	23.654443551075243
43	24.10050870856926	25.508157747895073	26.765770558197367	23.625562985338302
44	24.004077413436338	25.478467641665	26.7942004519072	23.723254492991465
45	23.941070960313652	25.28448254422052	26.840039639108515	23.934406856357306
46	24.05890083095576	25.461642552653096	26.642770477910975	23.836686138480164
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10456.0
1	6918.5
2	3381.0
3	3272.5
4	3164.0
5	3415.5
6	3667.0
7	3667.0
8	4431.5
9	5196.0
10	6749.0
11	8302.0
12	12385.0
13	16468.0
14	21885.5
15	27303.0
16	37905.5
17	48508.0
18	67382.0
19	86256.0
20	86256.0
21	113062.5
22	139869.0
23	176622.0
24	213375.0
25	393256.5
26	573138.0
27	482901.0
28	392664.0
29	439550.0
30	486436.0
31	486436.0
32	552069.0
33	617702.0
34	695000.0
35	772298.0
36	862913.0
37	953528.0
38	1052501.5
39	1151475.0
40	1257439.0
41	1363403.0
42	1474957.0
43	1586511.0
44	1586511.0
45	1692086.5
46	1797662.0
47	1877674.5
48	1957687.0
49	1994471.5
50	2031256.0
51	2006294.5
52	1981333.0
53	1907653.0
54	1833973.0
55	1726501.0
56	1619029.0
57	1619029.0
58	1462367.5
59	1305706.0
60	1156006.0
61	1006306.0
62	875612.5
63	744919.0
64	630447.5
65	515976.0
66	427028.5
67	338081.0
68	262677.0
69	187273.0
70	187273.0
71	140001.5
72	92730.0
73	69124.0
74	45518.0
75	34978.0
76	24438.0
77	17702.5
78	10967.0
79	7808.0
80	4649.0
81	4649.0
82	3363.0
83	2077.0
84	1483.0
85	889.0
86	716.5
87	544.0
88	300.5
89	57.0
90	42.5
91	28.0
92	18.0
93	8.0
94	8.0
95	6.5
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	2.3964212E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.01474970780301
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.44747346729234	28.07355992392483
2	15.36924599755275	12.607315555745583
3	5.725058288596443	7.044354983080986
4	2.805549770314878	4.602756864889958
5	1.6266897673832725	3.335913683073461
6	1.0886438370330642	2.6790272698112045
7	0.7379546738598648	2.1186918370846026
8	0.5483866967275417	1.7993554487495196
9	0.44402462613251736	1.6390403014433408
>10	2.923894832781056	23.594217294826983
>50	0.21658164627959847	5.9757767595400235
>100	0.06371740558229234	4.28920469450073
>500	0.0022675791755146575	0.6158012313244101
>1k	4.4005404831747843E-4	0.2796055420247808
>5k	3.05816745151435E-5	0.11050380585038973
>10k+	4.0775566020190884E-5	1.2348748041292363
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	254036	1.0600640655323865	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0013353245247538286	0.0	0.0	0.0	0.0
2	0.0013394974138936846	0.0	0.0	4.172889139855715E-6	1.669155655942286E-5
3	0.0013436703030335403	0.0	4.172889139855715E-6	4.172889139855715E-6	5.007466967826858E-5
4	0.0013520160813132516	0.0	8.34577827971143E-6	4.172889139855715E-6	7.928489365725858E-5
5	0.0013561889704531073	0.0	8.34577827971143E-6	8.34577827971143E-6	8.763067193697001E-5
6	0.0013853991944320974	0.0	8.34577827971143E-6	8.34577827971143E-6	1.0432222849639288E-4
7	0.0013937449727118088	0.0	8.34577827971143E-6	8.34577827971143E-6	1.0432222849639288E-4
8	0.0013937449727118088	0.0	8.34577827971143E-6	8.34577827971143E-6	1.2101378505581573E-4
9	0.001406263640131376	0.0	8.34577827971143E-6	8.34577827971143E-6	1.3353245247538288E-4
10	0.001418782307550943	0.0	8.34577827971143E-6	8.34577827971143E-6	1.418782307550943E-4
11	0.0015231045360473358	0.0	8.34577827971143E-6	1.2518667419567144E-5	1.5439689817466144E-4
12	0.0015314503143270474	0.0	8.34577827971143E-6	1.2518667419567144E-5	1.8360712215365144E-4
13	0.0015439689817466145	0.0	8.34577827971143E-6	1.2518667419567144E-5	2.1699023527249717E-4
14	0.0015731792057256046	0.0	1.2518667419567144E-5	1.2518667419567144E-5	2.295089026920643E-4
15	0.0015898707622850274	0.0	1.2518667419567144E-5	1.2518667419567144E-5	2.4202757011163145E-4
16	0.0016107352079843059	0.0	1.669155655942286E-5	1.2518667419567144E-5	2.837564615101886E-4
17	0.0016858472125017088	0.0	2.0864445699278573E-5	1.2518667419567144E-5	3.004480180696115E-4
18	0.0017108845473408432	0.0	2.0864445699278573E-5	1.2518667419567144E-5	3.046209072094672E-4
19	0.0017359218821799774	0.0	2.0864445699278573E-5	1.2518667419567144E-5	3.380040203283129E-4
20	0.001798515219277813	0.0	2.0864445699278573E-5	1.2518667419567144E-5	3.630413551674472E-4
21	0.0018485898889560816	0.0	2.0864445699278573E-5	1.2518667419567144E-5	3.7556002258701433E-4
22	0.0019237018934734845	0.0	2.0864445699278573E-5	1.669155655942286E-5	4.0894313570586006E-4
23	0.001990468119711176	0.0	2.0864445699278573E-5	1.669155655942286E-5	4.3398047054499434E-4
24	0.0020530614568090117	0.0	2.0864445699278573E-5	1.669155655942286E-5	4.464991379645615E-4
25	0.002107309015627136	4.172889139855715E-6	2.0864445699278573E-5	2.0864445699278573E-5	4.757093619435515E-4
26	0.002253360135522086	4.172889139855715E-6	2.0864445699278573E-5	2.0864445699278573E-5	4.9657380764283E-4
27	0.0023368179183192003	4.172889139855715E-6	2.0864445699278573E-5	2.0864445699278573E-5	5.132653642022529E-4
28	0.002428621479396026	4.172889139855715E-6	2.0864445699278573E-5	2.0864445699278573E-5	5.216111424819643E-4
29	0.002545462375311986	4.172889139855715E-6	2.0864445699278573E-5	2.0864445699278573E-5	5.341298099015315E-4
30	0.00265813038208809	4.172889139855715E-6	2.503733483913429E-5	2.0864445699278573E-5	5.591671447406658E-4
31	0.0027791441671439062	4.172889139855715E-6	2.9210223978990004E-5	2.0864445699278573E-5	5.675129230203772E-4
32	0.0030545548503743834	4.172889139855715E-6	3.338311311884572E-5	2.0864445699278573E-5	6.217604818385016E-4
33	0.003492708210059233	4.172889139855715E-6	3.338311311884572E-5	2.0864445699278573E-5	6.384520383979243E-4
34	0.0036930068887723075	4.172889139855715E-6	3.338311311884572E-5	2.0864445699278573E-5	6.384520383979243E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	108200	0.0	36.748615	1
AGCAGTG	116230	0.0	34.216637	2
TCAACGC	116885	0.0	34.098473	12
CAACGCA	117410	0.0	33.968147	13
AGTGGTA	117865	0.0	33.769142	5
ATCAACG	118265	0.0	33.725952	11
GTGGTAT	118060	0.0	33.716755	6
GGTATCA	118320	0.0	33.705208	8
AACGCAG	118415	0.0	33.7035	14
GTATCAA	118610	0.0	33.643032	9
ACGCAGA	118690	0.0	33.616985	15
TGGTATC	119020	0.0	33.470005	7
TATCAAC	119510	0.0	33.356205	10
GCAGTGG	120150	0.0	33.108616	3
AGAGTAC	119230	0.0	33.097378	19
CGCAGAG	120715	0.0	33.063	16
CAGTGGT	120495	0.0	32.947422	4
CAGAGTA	122075	0.0	32.41286	18
GCAGAGT	124250	0.0	32.01449	17
ACATGGG	86900	0.0	31.751438	24
>>END_MODULE