FastQCFastQC Report
Thu 26 May 2016
SRR805191_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805191_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13387400
Sequences flagged as poor quality0
Sequence length46
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT4101003.063328204132244No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGC353380.2639646234519026No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGG250650.18722828928694146No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC183980.13742773055260918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1877450.038.428721
TCAACGC1947950.037.38186312
AGCAGTG1931050.037.3786282
CAACGCA1950150.037.3448213
AGTGGTA1942600.037.287145
GTGGTAT1944500.037.2671626
AACGCAG1955350.037.25982314
ATCAACG1956700.037.23309711
ACGCAGA1956000.037.22290415
GGTATCA1953750.037.222788
GTATCAA1955900.037.1961759
TGGTATC1952000.037.180337
TATCAAC1958950.037.09844610
AGAGTAC1940650.037.02058419
CGCAGAG1968900.036.98816716
CAGTGGT1950300.036.9020164
GCAGTGG1959200.036.8425873
CAGAGTA1961650.036.74049818
GCAGAGT1983200.036.59237317
ACATGGG1430300.036.55596524