FastQCFastQC Report
Thu 26 May 2016
SRR805190_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805190_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21166793
Sequences flagged as poor quality0
Sequence length46
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT13809566.524162635312774No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGC1341540.6337946423910321No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGG571420.2699605934635445No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGAGAGATGGC376850.17803830745640117No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT311580.14720227102896505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3210300.038.418841
AGCAGTG3322250.037.1230352
AGTGGTA3339350.036.958695
GTGGTAT3347050.036.890996
TCAACGC3363750.036.83151212
ATCAACG3367250.036.8104511
GAGTACT1608400.036.7980620
CAACGCA3366050.036.79327413
GTATCAA3368300.036.7811669
GGTATCA3370450.036.738128
TATCAAC3368850.036.7347910
GCAGTGG3359000.036.696643
ACGCAGA3374800.036.6866215
AACGCAG3379100.036.6647914
TGGTATC3373100.036.6452267
CGCAGAG3381100.036.6117516
CAGTGGT3365000.036.5616654
AGAGTAC3358250.036.55713719
GTACTTT1614750.036.46632822
CAGAGTA3385200.036.37244418
Produced by FastQC (version 0.11.5)