##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805190_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21166793
Sequences flagged as poor quality	0
Sequence length	46
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.83682960380441	35.0	35.0	35.0	33.0	37.0
2	35.57473085318121	35.0	35.0	37.0	35.0	37.0
3	37.8171143356483	39.0	37.0	39.0	35.0	39.0
4	37.74948075506762	39.0	38.0	39.0	35.0	39.0
5	37.773188267112545	39.0	38.0	39.0	35.0	39.0
6	37.7136362131004	39.0	38.0	39.0	35.0	39.0
7	37.68361593558363	39.0	37.0	39.0	35.0	39.0
8	38.68692498669969	40.0	39.0	40.0	36.0	40.0
9	38.72278072545048	40.0	39.0	40.0	36.0	40.0
10	38.47501395227893	40.0	38.0	40.0	36.0	40.0
11	38.65368721657551	40.0	39.0	40.0	36.0	40.0
12	38.70043232340393	40.0	39.0	40.0	36.0	40.0
13	38.76745863201856	40.0	39.0	40.0	36.0	40.0
14	38.75411107388824	40.0	39.0	40.0	36.0	40.0
15	38.7448126884408	40.0	39.0	40.0	36.0	40.0
16	38.69788162996633	40.0	39.0	40.0	36.0	40.0
17	38.6898665281982	40.0	39.0	40.0	36.0	40.0
18	38.668744858987374	40.0	39.0	40.0	36.0	40.0
19	38.612729335048535	40.0	39.0	40.0	36.0	40.0
20	38.543300678567604	40.0	39.0	40.0	36.0	40.0
21	38.52306232691934	40.0	39.0	40.0	36.0	40.0
22	38.56128573657804	40.0	39.0	40.0	36.0	40.0
23	38.221075767122585	40.0	38.0	40.0	35.0	40.0
24	38.32109394181726	40.0	38.0	40.0	35.0	40.0
25	38.43016006250923	40.0	39.0	40.0	35.0	40.0
26	38.44696303308678	40.0	39.0	40.0	35.0	40.0
27	38.461484363739	40.0	39.0	40.0	35.0	40.0
28	38.412089729417204	40.0	39.0	40.0	35.0	40.0
29	38.37624991183124	40.0	39.0	40.0	35.0	40.0
30	38.37082825915102	40.0	38.0	40.0	35.0	40.0
31	38.30472641745965	40.0	38.0	40.0	35.0	40.0
32	38.285331982034315	40.0	38.0	40.0	35.0	40.0
33	38.12161299068782	40.0	38.0	40.0	34.0	40.0
34	38.138280985693015	40.0	38.0	40.0	35.0	40.0
35	38.07256819679769	40.0	38.0	40.0	34.0	40.0
36	37.9743184052492	40.0	38.0	40.0	34.0	40.0
37	37.707847853947456	40.0	37.0	40.0	34.0	40.0
38	37.67916108028269	40.0	37.0	40.0	33.0	40.0
39	37.68685090840167	40.0	37.0	40.0	33.0	40.0
40	37.60744582327611	40.0	37.0	40.0	33.0	40.0
41	37.5249178276558	40.0	36.0	40.0	33.0	40.0
42	37.413440193797896	40.0	36.0	40.0	33.0	40.0
43	37.31054118590379	40.0	36.0	40.0	33.0	40.0
44	37.34788241185143	40.0	36.0	40.0	33.0	40.0
45	37.2699626721913	40.0	36.0	40.0	33.0	40.0
46	37.12042745445661	40.0	35.0	40.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	4.0
12	12.0
13	27.0
14	90.0
15	242.0
16	503.0
17	967.0
18	1756.0
19	2790.0
20	4627.0
21	7710.0
22	12793.0
23	20342.0
24	31233.0
25	45326.0
26	64514.0
27	89220.0
28	118073.0
29	153178.0
30	196990.0
31	249260.0
32	313973.0
33	396948.0
34	516051.0
35	696437.0
36	1005677.0
37	1765887.0
38	4112953.0
39	1.1359208E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.14493952862864	44.550433313161804	21.283984777476682	19.020642380732877
2	23.905836845477726	43.918448108790024	15.440581858574417	16.735133187157828
3	36.93634647440451	24.572957273215646	19.303250142806235	19.187446109573614
4	20.995882560008027	22.555037033715973	20.18564172664229	36.26343867963371
5	20.209084106411396	37.92691694013354	22.288785079534723	19.575213873920344
6	35.35427875162761	22.9264678876956	21.237681116832388	20.481572243844404
7	21.741323780130507	22.412625285275855	36.19539814085204	19.650652793741592
8	34.23514842328736	23.78194939592408	22.460110041232983	19.522792139555577
9	34.41682450430729	23.157230289916853	22.859967497201865	19.565977708573993
10	19.95679269882783	23.55408776379114	36.8294715217369	19.659648015644127
11	19.667740880727656	37.82430337935463	22.912209705079086	19.595746034838626
12	19.62863717711039	23.397191062434448	37.11549501145497	19.85867674900019
13	19.520524436554936	23.662271370065366	22.59559584675865	34.22160834662105
14	19.74460656368681	38.25107563531235	22.399302530147104	19.605015270853738
15	19.758014357678086	37.88565891866567	22.449574671042516	19.906752052613733
16	19.9710650545881	23.255062776869412	22.54376938443155	34.23010278411095
17	34.32381561061234	23.133707595666475	22.70909910632187	19.833377687399313
18	19.854694095605318	23.352285818640546	22.67366624693689	34.11935383881725
19	20.010343560311664	37.78637604666895	22.52173959465659	19.681540798362793
20	34.51304597725314	23.45759700111396	22.651778188599472	19.37757883303342
21	20.144440397749435	37.55280263760316	22.71443765713587	19.588319307511533
22	34.31260937828418	23.195134000696278	22.85983993890808	19.632416682111455
23	20.022272622971276	23.389991105407418	36.957214066391636	19.630522205229674
24	19.817357310576053	37.39345398237702	22.72373051505724	20.065458191989688
25	19.80311802548454	23.393080850745786	22.70337788062651	34.10042324314316
26	20.111105163640048	30.548165704648788	29.75520665790042	19.585522473810745
27	20.320007853811394	22.978280176878943	37.1894221292758	19.512289840033866
28	27.661814427910734	22.973673905158897	29.80005048473805	19.564461182192314
29	27.75916030359441	22.91443488864846	29.793346587742413	19.533058220014716
30	27.63213586488988	22.931135576371915	29.81034963586595	19.626378922872256
31	25.36300610111319	23.95133263692804	30.280657065054683	20.405004196904084
32	23.841051405378227	25.03926787586575	30.8008114408262	20.31886927792982
33	23.738834692624433	24.40012523389821	30.98241665612736	20.87862341735
34	22.663622212396557	24.339407486056107	31.20894128836617	21.788029013181166
35	21.943517848924966	24.362372703318826	32.197999952094776	21.496109495661436
36	22.548404002439103	24.52313394853911	31.724640572617684	21.2038214764041
37	23.116817932692967	24.733609857667147	31.368719862286177	20.780852347353708
38	22.670080441567126	24.78559222457554	31.796035422087797	20.748291911769535
39	22.8506746392805	24.626607346705757	31.52124651098539	21.001471503028352
40	22.17478103555886	25.639736732909896	31.10430096803044	21.081181263500806
41	22.719653374037343	25.352654981791527	31.077631835866683	20.85005980830445
42	22.43362515993802	25.531756275029476	31.236167897517586	20.798450667514913
43	22.16140631223634	24.996375218484918	32.11374533685854	20.728473132420202
44	22.511979022991344	24.80351652704309	31.715494170515107	20.96901027945046
45	22.957818881679433	24.44045727664082	31.388212659329167	21.21351118235058
46	22.269093858479174	24.542300763275758	31.198632688475765	21.9899726897693
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	21288.0
1	14325.0
2	7362.0
3	6777.5
4	6193.0
5	6782.0
6	7371.0
7	7371.0
8	8463.5
9	9556.0
10	12876.0
11	16196.0
12	18691.0
13	21186.0
14	26852.5
15	32519.0
16	41242.5
17	49966.0
18	64582.0
19	79198.0
20	79198.0
21	109546.5
22	139895.0
23	187273.5
24	234652.0
25	979579.5
26	1724507.0
27	1047528.5
28	370550.0
29	386649.0
30	402748.0
31	402748.0
32	459903.5
33	517059.0
34	590055.5
35	663052.0
36	745529.5
37	828007.0
38	920180.0
39	1012353.0
40	1117035.5
41	1221718.0
42	1312868.5
43	1404019.0
44	1404019.0
45	1496883.5
46	1589748.0
47	1649339.5
48	1708931.0
49	1744535.0
50	1780139.0
51	1730971.0
52	1681803.0
53	1593734.5
54	1505666.0
55	1501677.0
56	1497688.0
57	1497688.0
58	1237854.5
59	978021.0
60	814575.5
61	651130.0
62	542944.5
63	434759.0
64	348433.0
65	262107.0
66	206047.0
67	149987.0
68	116743.0
69	83499.0
70	83499.0
71	61609.5
72	39720.0
73	28788.0
74	17856.0
75	13268.0
76	8680.0
77	6654.0
78	4628.0
79	3198.5
80	1769.0
81	1769.0
82	1278.5
83	788.0
84	526.5
85	265.0
86	207.0
87	149.0
88	90.0
89	31.0
90	24.0
91	17.0
92	12.5
93	8.0
94	8.0
95	6.5
96	5.0
97	4.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	2.1166793E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.91000976385241
#Duplication Level	Percentage of deduplicated	Percentage of total
1	33.74996670965613	7.057121334286035
2	19.537903877509315	8.170755216878595
3	13.640615478538109	8.556762085235645
4	8.945025269003231	7.481622628510564
5	6.035536265175241	6.3101561117449805
6	4.3137273148592215	5.412004816338191
7	3.0148993126189136	4.412910184472539
8	2.1271977874933623	3.5583781204905156
9	1.5755230296956997	2.9649781740700267
>10	6.482518817648342	22.401163639042515
>50	0.3422042372662988	4.936042199283787
>100	0.2270936954864414	8.39047180754736
>500	0.004834209466330981	0.7045754539728141
>1k	0.0025258217198385596	0.9904146825267817
>5k	9.014186599509897E-5	0.1703825794107909
>10k+	3.3803199748162115E-4	8.482260966188916
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	1380956	6.524162635312774	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGC	134154	0.6337946423910321	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGG	57142	0.2699605934635445	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGAGAGATGGC	37685	0.17803830745640117	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	31158	0.14720227102896505	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4094344381787075E-4	0.0	0.0	0.0	0.0
2	0.003146437913386312	0.0	0.0	0.0	0.0
3	0.0031606110571403044	0.0	0.0	0.0	0.0
4	0.004114936069909126	0.0	0.0	0.0	0.0
5	0.004157455501171103	0.0	0.0	0.0	0.0
6	0.004162179882422434	0.0	0.0	0.0	0.0
7	0.004166904263673765	0.0	0.0	0.0	0.0
8	0.004166904263673765	0.0	0.0	0.0	0.0
9	0.004166904263673765	0.0	0.0	0.0	0.0
10	0.004166904263673765	0.0	0.0	0.0	0.0
11	0.004166904263673765	0.0	0.0	0.0	0.0
12	0.004171628644925095	0.0	0.0	0.0	0.0
13	0.004181077407427757	0.0	0.0	0.0	0.0
14	0.004190526169930419	0.0	0.0	0.0	0.0
15	0.004204699313684411	9.448762502661599E-6	0.0	0.0	0.0
16	0.0042235968386897344	9.448762502661599E-6	0.0	0.0	0.0
17	0.0042235968386897344	9.448762502661599E-6	0.0	0.0	0.0
18	0.0042283212199410655	9.448762502661599E-6	0.0	0.0	0.0
19	0.004237769982443727	9.448762502661599E-6	0.0	0.0	0.0
20	0.004237769982443727	9.448762502661599E-6	0.0	0.0	0.0
21	0.0042661162699517115	9.448762502661599E-6	0.0	0.0	0.0
22	0.00431336008246502	9.448762502661599E-6	0.0	0.0	0.0
23	0.004346430751224335	9.448762502661599E-6	0.0	0.0	0.0
24	0.004398398944988974	9.448762502661599E-6	0.0	9.448762502661599E-6	0.0
25	0.004436193994999621	9.448762502661599E-6	0.0	9.448762502661599E-6	0.0
26	0.004502335332518251	9.448762502661599E-6	0.0	9.448762502661599E-6	0.0
27	0.004573201051288214	9.448762502661599E-6	0.0	9.448762502661599E-6	0.0
28	0.004587374195042206	9.448762502661599E-6	0.0	9.448762502661599E-6	0.0
29	0.004686586201320153	9.448762502661599E-6	0.0	9.448762502661599E-6	0.0
30	0.0048377664013627385	9.448762502661599E-6	0.0	9.448762502661599E-6	0.0
31	0.004894458976378708	9.448762502661599E-6	0.0	9.448762502661599E-6	0.0
32	0.005064536701426617	9.448762502661599E-6	0.0	9.448762502661599E-6	0.0
33	0.005210992520217872	9.448762502661599E-6	0.0	9.448762502661599E-6	0.0
34	0.005428314057779088	9.448762502661599E-6	0.0	1.8897525005323197E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	321030	0.0	38.41884	1
AGCAGTG	332225	0.0	37.123035	2
AGTGGTA	333935	0.0	36.95869	5
GTGGTAT	334705	0.0	36.89099	6
TCAACGC	336375	0.0	36.831512	12
ATCAACG	336725	0.0	36.81045	11
GAGTACT	160840	0.0	36.79806	20
CAACGCA	336605	0.0	36.793274	13
GTATCAA	336830	0.0	36.781166	9
GGTATCA	337045	0.0	36.73812	8
TATCAAC	336885	0.0	36.73479	10
GCAGTGG	335900	0.0	36.69664	3
ACGCAGA	337480	0.0	36.68662	15
AACGCAG	337910	0.0	36.66479	14
TGGTATC	337310	0.0	36.645226	7
CGCAGAG	338110	0.0	36.61175	16
CAGTGGT	336500	0.0	36.561665	4
AGAGTAC	335825	0.0	36.557137	19
GTACTTT	161475	0.0	36.466328	22
CAGAGTA	338520	0.0	36.372444	18
>>END_MODULE