FastQCFastQC Report
Thu 26 May 2016
SRR805189_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805189_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16025013
Sequences flagged as poor quality0
Sequence length46
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT163491710.202281895184734No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGC386220.24101072492109676No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC310140.19353494440222918No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGG239720.14959114229735726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT4626550.039.404311
AGCAGTG4688600.038.8678932
TCAACGC4692450.038.57259812
CAACGCA4696200.038.55840713
AGTGGTA4705150.038.5556265
GTGGTAT4705300.038.532726
GCAGTGG4728700.038.5175633
TGGTATC4707700.038.494387
GAGTACT1894600.038.4777820
ACGCAGA4704900.038.47775715
GTATCAA4702850.038.4707159
GGTATCA4717000.038.4680948
AACGCAG4707700.038.46761714
ATCAACG4722350.038.45034811
TATCAAC4703600.038.44034210
CGCAGAG4728450.038.4295116
CAGTGGT4719150.038.428114
AGAGTAC4708050.038.34921319
GTACTTT1887400.038.30242522
CAGAGTA4713600.038.290918