FastQCFastQC Report
Thu 26 May 2016
SRR805188_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805188_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13544908
Sequences flagged as poor quality0
Sequence length46
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT2278731.6823517738178806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT679550.037.0009571
AGCAGTG726500.034.5905042
AGTGGTA767550.032.594625
CAACGCA766450.032.5735513
TCAACGC766050.032.56184412
ATCAACG772000.032.4274611
GCAGTGG775300.032.4158363
GTGGTAT771300.032.4076236
ACGCAGA772250.032.3548115
AACGCAG773350.032.29068414
GGTATCA775650.032.218148
TGGTATC778400.032.0966077
GTATCAA778350.032.0832569
TATCAAC780150.032.00410510
CAGTGGT781550.031.9979524
AGAGTAC778300.031.87716919
CGCAGAG787250.031.82470716
GAGTACT325800.031.40577120
CAGAGTA796350.031.17975818
GCAGAGT811350.030.79805417
Produced by FastQC (version 0.11.5)