##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805188_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13544908
Sequences flagged as poor quality	0
Sequence length	46
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.53160634239819	37.0	35.0	37.0	33.0	37.0
2	35.76687098945227	37.0	35.0	37.0	35.0	37.0
3	37.69243703980861	39.0	38.0	39.0	35.0	39.0
4	37.74520026271127	39.0	38.0	39.0	35.0	39.0
5	37.78679751829986	39.0	38.0	39.0	35.0	39.0
6	37.80702785135196	39.0	38.0	39.0	35.0	39.0
7	37.741941694989734	39.0	38.0	39.0	35.0	39.0
8	38.66884780612759	40.0	39.0	40.0	36.0	40.0
9	38.71671819402538	40.0	39.0	40.0	36.0	40.0
10	38.60594881855233	40.0	39.0	40.0	36.0	40.0
11	38.68568357939382	40.0	39.0	40.0	36.0	40.0
12	38.66803008185807	40.0	39.0	40.0	36.0	40.0
13	38.73986275875776	40.0	39.0	40.0	36.0	40.0
14	38.674513034713854	40.0	39.0	40.0	36.0	40.0
15	38.60322786983861	40.0	39.0	40.0	36.0	40.0
16	38.61920841396634	40.0	39.0	40.0	36.0	40.0
17	38.6598797865589	40.0	39.0	40.0	36.0	40.0
18	38.53321100446013	40.0	39.0	40.0	36.0	40.0
19	38.54987335462153	40.0	39.0	40.0	35.0	40.0
20	38.41280169640133	40.0	39.0	40.0	35.0	40.0
21	38.40525103603509	40.0	39.0	40.0	35.0	40.0
22	38.38658786017594	40.0	39.0	40.0	35.0	40.0
23	38.30104648920465	40.0	38.0	40.0	35.0	40.0
24	38.193309175669555	40.0	38.0	40.0	35.0	40.0
25	38.25469593444267	40.0	38.0	40.0	35.0	40.0
26	38.24279234676234	40.0	38.0	40.0	35.0	40.0
27	38.162675671182114	40.0	38.0	40.0	34.0	40.0
28	38.14546071483099	40.0	38.0	40.0	34.0	40.0
29	38.113220333427144	40.0	38.0	40.0	34.0	40.0
30	37.98735473138688	40.0	38.0	40.0	34.0	40.0
31	38.01677456945444	40.0	38.0	40.0	34.0	40.0
32	37.723456814915245	40.0	38.0	40.0	33.0	40.0
33	37.804586638757534	40.0	38.0	40.0	33.0	40.0
34	37.85066860550105	40.0	38.0	40.0	34.0	40.0
35	37.81423587373203	40.0	38.0	40.0	34.0	40.0
36	37.73317234786681	40.0	38.0	40.0	33.0	40.0
37	37.65344201673426	40.0	37.0	40.0	33.0	40.0
38	37.57985487978213	40.0	37.0	40.0	33.0	40.0
39	37.55995574130145	40.0	37.0	40.0	33.0	40.0
40	37.48795680265972	40.0	37.0	40.0	33.0	40.0
41	37.299121485358185	40.0	37.0	40.0	33.0	40.0
42	36.777787490324776	39.0	36.0	40.0	31.0	40.0
43	36.75181529472182	39.0	36.0	40.0	31.0	40.0
44	36.781926979496646	39.0	36.0	40.0	31.0	40.0
45	36.8443602570058	39.0	36.0	40.0	31.0	40.0
46	36.98685151645179	39.0	36.0	40.0	32.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	3.0
11	25.0
12	39.0
13	109.0
14	185.0
15	435.0
16	905.0
17	1502.0
18	2489.0
19	3889.0
20	6107.0
21	9201.0
22	13727.0
23	20122.0
24	28409.0
25	38938.0
26	52724.0
27	68699.0
28	88297.0
29	111894.0
30	140647.0
31	175528.0
32	218416.0
33	272935.0
34	348933.0
35	469417.0
36	670422.0
37	1108599.0
38	2489495.0
39	7202813.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.90832776420482	29.724447002519323	25.140104310786015	21.22712092248984
2	23.218791888435124	27.919480885362972	26.41161534651989	22.450111879682016
3	26.831182611207105	25.071724370516215	25.06941353902145	23.02767947925523
4	23.238083270849827	25.75340489577338	25.29481189536319	25.713699938013608
5	24.254856511391587	29.891188629704978	24.50554850575582	21.348406353147617
6	27.82194607744844	24.861660189940014	25.42567288017017	21.890720852441376
7	22.914869558360973	25.18860224078303	30.028952577603334	21.867575623252662
8	27.295423490510235	25.09461120001701	25.381235516697494	22.228729792775262
9	26.80462650613795	25.294760215425605	25.668908197826074	22.231705080610368
10	22.128079422909334	25.435661873820038	30.433768911534877	22.00248979173576
11	22.055653681811645	29.877227663709494	25.792401100103447	22.274717554375414
12	22.0553288364897	25.356539889381306	30.132917846322766	22.45521342780623
13	22.07085496630911	25.578032719011457	25.481944949349234	26.869167365330206
14	22.24604257186538	29.942861184439202	25.496592520229743	22.314503723465677
15	22.53851410434091	29.487221323319435	25.414436185170103	22.559828387169556
16	22.427232433029445	25.18604777529681	25.39758114267	26.989138649003742
17	26.989625916986665	25.171215633210647	25.426189679545995	22.41296877025669
18	22.552209287800256	25.150004710257168	25.422217707200375	26.8755682947422
19	22.68803892946338	29.80966721959278	25.308152702107684	22.194141148836152
20	27.15869314136353	25.04758245681698	25.50233637614962	22.291388025669868
21	22.530119805907873	29.511119603027204	25.567120869333333	22.39163972173159
22	27.04586107192459	25.059173528531904	25.543067549812815	22.35189784973069
23	22.45655710618337	25.135571241975214	29.939745622487802	22.468126029353613
24	22.426457233965706	29.518642725369563	25.396119338721242	22.65878070194349
25	22.620810713516843	25.0045035374179	25.505400258163434	26.869285490901824
26	22.8291916046975	27.488226571933893	27.413763164725815	22.268818658642793
27	22.895696301517884	24.790511681585432	29.921340181860224	22.392451835036457
28	25.625917872605708	24.91090378760786	27.206785014708114	22.256393325078324
29	25.505481469493922	24.902288003728042	27.216744476965072	22.375486049812963
30	25.438555950324655	24.850120798162674	27.23977896343039	22.47154428808228
31	24.3678805348844	25.492923244661387	27.408144817225775	22.73105140322843
32	23.813465547348127	25.733655776768654	27.668161348899527	22.78471732698369
33	23.483120003472894	25.509025236642437	27.770546688098584	23.237308071786092
34	23.39077533786128	25.57364730716517	27.904545383401647	23.1310319715719
35	23.276459315928907	25.57471043730973	27.946539024111495	23.20229122264987
36	23.28358376446706	25.581406680650765	27.887476238302984	23.24753331657919
37	23.552548308190797	25.578992489280843	27.760188552037413	23.108270650490944
38	23.518026109885724	25.649794003768793	27.771344035706996	23.060835850638483
39	23.53821819978401	25.537700219152466	27.69934649980642	23.2247350812571
40	23.494917794938143	25.710798478660763	27.611357714648193	23.182926011752905
41	23.424765971094082	25.84093594434159	27.637492997368458	23.096805087195868
42	23.524552547717565	25.866598724775393	27.466919671953477	23.14192905555357
43	23.565874349238843	25.66321602184378	27.67929468402443	23.091614944892942
44	23.60487055356891	25.603451865453792	27.656171603380397	23.135505977596896
45	23.479111116886138	25.470848528465456	27.65925025109067	23.390790103557734
46	23.522167887740544	25.62521650202423	27.516643154756014	23.335972455479208
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16515.0
1	10846.0
2	5177.0
3	4642.0
4	4107.0
5	4351.0
6	4595.0
7	4595.0
8	5379.5
9	6164.0
10	7958.5
11	9753.0
12	13652.0
13	17551.0
14	22099.5
15	26648.0
16	34889.0
17	43130.0
18	56526.0
19	69922.0
20	69922.0
21	88177.5
22	106433.0
23	130410.5
24	154388.0
25	305599.5
26	456811.0
27	360443.0
28	264075.0
29	283598.0
30	303121.0
31	303121.0
32	337590.5
33	372060.0
34	410517.5
35	448975.0
36	493395.0
37	537815.0
38	588508.0
39	639201.0
40	694566.5
41	749932.0
42	807948.0
43	865964.0
44	865964.0
45	926241.5
46	986519.0
47	1032120.5
48	1077722.0
49	1098412.5
50	1119103.0
51	1104963.0
52	1090823.0
53	1046601.0
54	1002379.0
55	943369.5
56	884360.0
57	884360.0
58	798403.5
59	712447.0
60	628392.0
61	544337.0
62	470715.0
63	397093.0
64	334133.5
65	271174.0
66	222066.5
67	172959.0
68	134535.5
69	96112.0
70	96112.0
71	71213.0
72	46314.0
73	33902.0
74	21490.0
75	16595.5
76	11701.0
77	8203.0
78	4705.0
79	3270.0
80	1835.0
81	1835.0
82	1340.0
83	845.0
84	608.0
85	371.0
86	302.5
87	234.0
88	133.5
89	33.0
90	20.0
91	7.0
92	5.0
93	3.0
94	3.0
95	1.5
96	0.0
97	2.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1.3544908E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.205926151719964
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.746650684191536	23.619634990694944
2	19.224980382612117	15.459162830631357
3	8.91083791407409	10.748054733696277
4	4.315324072078862	6.94006403850969
5	2.3574445718754804	4.739162118179934
6	1.4067677825162352	3.3936240945879965
7	0.912151598198172	2.567172985743034
8	0.6444749566297847	2.072937001031204
9	0.47420800915830935	1.7159374977095818
>10	2.875982125247035	21.003085015894058
>50	0.0979038039564999	2.5877431564608027
>100	0.03188805066320398	2.3763521256415303
>500	9.65727333050606E-4	0.2497901000514272
>1k	2.6020092014882116E-4	0.15915257711221587
>5k	8.00602727891042E-5	0.23982395193775052
>10k+	8.00602727891042E-5	2.128302782118264
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	227873	1.6823517738178806	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.001240318501978751	7.382848226063994E-6	0.0	7.382848226063994E-6	3.691424113031997E-5
2	0.0012698498948830069	7.382848226063994E-6	0.0	2.9531392904255977E-5	4.4297089356383966E-5
3	0.001291998439561199	7.382848226063994E-6	0.0	3.691424113031997E-5	4.4297089356383966E-5
4	0.001291998439561199	1.4765696452127989E-5	0.0	4.4297089356383966E-5	6.644563403457595E-5
5	0.0013067641360133269	2.2148544678191983E-5	0.0	5.9062785808511954E-5	8.121133048670393E-5
6	0.0013067641360133269	2.2148544678191983E-5	0.0	6.644563403457595E-5	8.859417871276793E-5
7	0.0013067641360133269	3.691424113031997E-5	0.0	8.121133048670393E-5	1.0335987516489591E-4
8	0.001314146984239391	5.1679937582447957E-5	0.0	8.859417871276793E-5	1.0335987516489591E-4
9	0.001321529832465455	5.9062785808511954E-5	0.0	1.107427233909599E-4	1.1812557161702391E-4
10	0.0013362955289175828	6.644563403457595E-5	0.0	1.107427233909599E-4	1.1812557161702391E-4
11	0.001417506859404287	7.382848226063994E-5	0.0	1.107427233909599E-4	1.4027411629521588E-4
12	0.0014248897076303507	7.382848226063994E-5	0.0	1.255084198430879E-4	1.6242266097340785E-4
13	0.0014396554040824788	7.382848226063994E-5	0.0	1.255084198430879E-4	2.0671975032979183E-4
14	0.0014691867969867348	1.0335987516489591E-4	0.0	1.328912680691519E-4	2.214854467819198E-4
15	0.0014691867969867348	1.0335987516489591E-4	0.0	1.328912680691519E-4	2.8054823259043176E-4
16	0.0014913353416649267	1.255084198430879E-4	0.0	1.328912680691519E-4	3.0269677726862374E-4
17	0.0015356324310213108	1.328912680691519E-4	0.0	1.328912680691519E-4	3.248453219468157E-4
18	0.0015577809756995027	1.5503981274734386E-4	0.0	1.5503981274734386E-4	3.248453219468157E-4
19	0.0015799295203776948	1.6242266097340785E-4	0.0	1.6980550919947187E-4	3.3222817017287975E-4
20	0.0016242266097340786	1.8457120565159985E-4	0.0	1.9195405387766384E-4	3.3961101839894374E-4
21	0.0016759065473165265	1.8457120565159985E-4	0.0	1.9195405387766384E-4	3.4699386662500773E-4
22	0.0017128207884468465	1.8457120565159985E-4	0.0	1.9933690210372784E-4	3.912909559813917E-4
23	0.0017423521813511025	1.8457120565159985E-4	0.0	1.9933690210372784E-4	3.9867380420745567E-4
24	0.0018235635118378066	1.8457120565159985E-4	0.0	2.0671975032979183E-4	4.2082234888564764E-4
25	0.0018826262976463185	1.9195405387766384E-4	0.0	2.1410259855585582E-4	4.2082234888564764E-4
26	0.0019712204763590864	1.9933690210372784E-4	0.0	2.1410259855585582E-4	4.2082234888564764E-4
27	0.0020229004139415345	2.0671975032979183E-4	0.0	2.214854467819198E-4	4.355880453377756E-4
28	0.0020598146550718545	2.214854467819198E-4	0.0	2.214854467819198E-4	4.429708935638396E-4
29	0.0021631745302367503	2.214854467819198E-4	0.0	2.436339914601118E-4	4.6511943824203164E-4
30	0.0022370030124973903	2.288682950079838E-4	0.0	2.436339914601118E-4	4.7250228646809563E-4
31	0.002303448646531966	2.3625114323404782E-4	0.0	2.510168396861758E-4	4.798851346941596E-4
32	0.002488019852183566	2.3625114323404782E-4	0.0	2.583996879122398E-4	5.315650722766076E-4
33	0.0029236078975213415	2.510168396861758E-4	0.0	2.7316538436436777E-4	5.537136169547996E-4
34	0.0030048192280080453	2.583996879122398E-4	0.0	2.8054823259043176E-4	5.758621616329915E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	67955	0.0	37.000957	1
AGCAGTG	72650	0.0	34.590504	2
AGTGGTA	76755	0.0	32.59462	5
CAACGCA	76645	0.0	32.57355	13
TCAACGC	76605	0.0	32.561844	12
ATCAACG	77200	0.0	32.42746	11
GCAGTGG	77530	0.0	32.415836	3
GTGGTAT	77130	0.0	32.407623	6
ACGCAGA	77225	0.0	32.35481	15
AACGCAG	77335	0.0	32.290684	14
GGTATCA	77565	0.0	32.21814	8
TGGTATC	77840	0.0	32.096607	7
GTATCAA	77835	0.0	32.083256	9
TATCAAC	78015	0.0	32.004105	10
CAGTGGT	78155	0.0	31.997952	4
AGAGTAC	77830	0.0	31.877169	19
CGCAGAG	78725	0.0	31.824707	16
GAGTACT	32580	0.0	31.405771	20
CAGAGTA	79635	0.0	31.179758	18
GCAGAGT	81135	0.0	30.798054	17
>>END_MODULE