FastQCFastQC Report
Thu 26 May 2016
SRR805187_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805187_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10752712
Sequences flagged as poor quality0
Sequence length46
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT1035230.9627617665199253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT481450.033.9723781
AGCAGTG522950.031.574722
AGTGGTA550050.030.0518155
TCAACGC554050.030.01173212
GTGGTAT552800.029.913176
CAACGCA556350.029.89485213
ATCAACG560100.029.7161211
AACGCAG560750.029.66027614
ACGCAGA560600.029.6432415
GGTATCA560300.029.6412648
GTATCAA562000.029.6085419
TGGTATC562900.029.4261847
GCAGTGG561350.029.4005533
TATCAAC566350.029.32815610
CGCAGAG568400.029.25052616
AGAGTAC560600.029.15091119
CAGTGGT565200.029.108284
CAGAGTA572850.028.66719218
GCAGAGT584950.028.28959717
ACATGGG405650.028.01922624
Produced by FastQC (version 0.11.5)