FastQCFastQC Report
Thu 26 May 2016
SRR805186_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805186_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13275117
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2324791.7512388026410615No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC229470.17285723357466454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1325750.044.5875171
AGCAGTG1400750.042.4316982
AGTGGTA1411200.041.9443055
CAACGCA1412600.041.85282513
TCAACGC1407100.041.8210112
GGTATCA1413750.041.8171168
GTGGTAT1409000.041.8146556
GTATCAA1416000.041.7772259
AACGCAG1417350.041.71753714
TGGTATC1413550.041.6734127
TATCAAC1421650.041.6657410
ACGCAGA1418600.041.64764415
GCAGTGG1425200.041.6246153
ATCAACG1420150.041.5459311
CAGTGGT1432150.041.3454974
CGCAGAG1429050.041.33980616
AGAGTAC1417550.041.28228419
CAGAGTA1431050.040.9601718
GCAGAGT1441550.040.67486217
ACATGGG1002500.040.2301224
Produced by FastQC (version 0.11.5)