##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805186_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13275117
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.11725990814243	37.0	37.0	37.0	35.0	37.0
2	36.200719059575896	37.0	37.0	37.0	35.0	37.0
3	37.98971730343318	39.0	39.0	39.0	35.0	39.0
4	37.94663173213464	39.0	38.0	39.0	35.0	39.0
5	37.90809919038755	39.0	38.0	39.0	35.0	39.0
6	37.887206794486254	39.0	38.0	39.0	35.0	39.0
7	37.78147296178256	39.0	38.0	39.0	35.0	39.0
8	38.69887489503859	40.0	39.0	40.0	36.0	40.0
9	38.591465973520236	40.0	39.0	40.0	36.0	40.0
10	38.457144520835485	40.0	38.0	40.0	36.0	40.0
11	38.54604159044323	40.0	38.0	40.0	36.0	40.0
12	38.3895106913182	40.0	38.0	40.0	35.0	40.0
13	38.60930189918477	40.0	39.0	40.0	36.0	40.0
14	38.63510980731846	40.0	39.0	40.0	36.0	40.0
15	38.58157348067064	40.0	39.0	40.0	36.0	40.0
16	38.55409168898474	40.0	38.0	40.0	36.0	40.0
17	38.16585270020596	40.0	38.0	40.0	34.0	40.0
18	38.25941737462653	40.0	38.0	40.0	35.0	40.0
19	38.33071753717877	40.0	38.0	40.0	35.0	40.0
20	38.27354809754219	40.0	38.0	40.0	35.0	40.0
21	38.2328857063934	40.0	38.0	40.0	35.0	40.0
22	38.15004960031614	40.0	38.0	40.0	35.0	40.0
23	37.64001176034833	39.0	37.0	40.0	34.0	40.0
24	37.67065314753911	39.0	38.0	40.0	34.0	40.0
25	37.6591736253624	39.0	38.0	40.0	34.0	40.0
26	37.448808323120616	39.0	37.0	40.0	33.0	40.0
27	37.452263358582826	39.0	37.0	40.0	33.0	40.0
28	37.37819742003027	39.0	37.0	40.0	33.0	40.0
29	37.1554434510822	39.0	37.0	40.0	33.0	40.0
30	37.080855558561176	38.0	37.0	40.0	33.0	40.0
31	36.90414284107628	38.0	36.0	40.0	32.0	40.0
32	36.670462716072485	38.0	36.0	40.0	31.0	40.0
33	36.57857358243999	38.0	36.0	40.0	31.0	40.0
34	36.44288378023335	38.0	35.0	40.0	31.0	40.0
35	36.18315266072608	38.0	35.0	40.0	31.0	40.0
36	35.980990073383154	38.0	35.0	39.0	30.0	40.0
37	35.794222981236246	38.0	35.0	39.0	30.0	40.0
38	35.743923688205534	38.0	35.0	39.0	30.0	40.0
39	35.61651230644521	38.0	35.0	39.0	30.0	40.0
40	35.51931828548103	37.0	34.0	38.0	30.0	40.0
41	35.28035203004237	37.0	34.0	38.0	30.0	40.0
42	35.07330895840692	37.0	34.0	38.0	29.0	40.0
43	34.76261324099818	36.0	34.0	38.0	29.0	40.0
44	34.62955053428154	36.0	33.0	38.0	28.0	39.0
45	34.50622725208373	36.0	33.0	38.0	28.0	39.0
46	34.42163560592347	36.0	33.0	38.0	28.0	39.0
47	34.17952082832867	36.0	33.0	38.0	28.0	39.0
48	34.01739751144943	36.0	33.0	38.0	28.0	39.0
49	33.64085009570914	35.0	32.0	38.0	27.0	39.0
50	33.399422393038044	35.0	32.0	38.0	26.0	39.0
51	33.05796943258579	35.0	31.0	37.0	26.0	39.0
52	32.58618097301892	34.0	31.0	37.0	25.0	38.0
53	34.65043065157166	37.0	33.0	39.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	5.0
12	12.0
13	80.0
14	269.0
15	715.0
16	1432.0
17	2354.0
18	3767.0
19	6157.0
20	10095.0
21	15596.0
22	22510.0
23	31210.0
24	41981.0
25	55284.0
26	71523.0
27	90817.0
28	113552.0
29	140761.0
30	175618.0
31	221276.0
32	285921.0
33	384018.0
34	547407.0
35	839865.0
36	1491851.0
37	3230019.0
38	5114213.0
39	376808.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.788006388192283	34.53030960103779	24.97352754028458	20.708156470485346
2	25.247242642004586	32.53590156681858	21.564435175976225	20.652420615200604
3	30.8132048855012	23.433209665873378	22.800944051943194	22.952641396682232
4	21.650882624989293	25.210331479564363	22.284617152526792	30.85416874291955
5	23.467936290128367	33.653782486436846	22.1598950879303	20.718386135504492
6	31.941277805687136	23.691437145149077	23.265331672782995	21.101953376380788
7	22.461157969455183	23.914953065950378	32.81853561064659	20.805353353947844
8	30.83532145140416	23.884866702116447	23.747685236973805	21.53212660950559
9	30.614231121277502	23.813952072889453	24.205549374819068	21.36626743101398
10	21.118412741673012	24.29014373281983	33.36428597955107	21.227157545956093
11	20.994850742181782	33.517203652517715	24.175297287398674	21.312648317901832
12	21.206909136846026	24.13691721135113	33.1852291772645	21.470944474538342
13	21.03422515974812	24.327152822833877	23.88632808283347	30.75229393458453
14	21.29448651940318	33.58053266121873	23.709644140989493	21.4153366783886
15	21.311058878049813	33.33260264297483	23.714374796094077	21.641963682881286
16	21.449355210955957	24.041543287339763	23.68477807012925	30.824323431575028
17	30.909430026115775	23.885070090154382	23.56116334040596	21.644336543323874
18	21.546672620663156	23.915081125085376	23.752016648892813	30.786229605358656
19	21.64394483302859	33.29049378623179	23.679331790446742	21.386229590292878
20	31.020321704132627	23.953129753959985	23.76045348602201	21.266095055885383
21	21.703394403228234	33.08068772576543	23.836957519847093	21.37896035115924
22	30.83288832783922	23.83489350790656	23.973483623534165	21.358734540720057
23	21.864372268809383	23.837997058707654	32.97651538589076	21.321115286592203
24	21.389762515840726	33.07085730393186	23.86553730562224	21.673842874605175
25	21.491546929492223	23.94526541649313	23.844143897187497	30.71904375682715
26	21.948831034784856	30.11595302700534	26.61800268878986	21.317213249419947
27	21.908703328189123	23.573283760888888	33.13980584879214	21.378207062129846
28	28.50654348281827	23.616921794361588	26.60955831877037	21.26697640404977
29	28.776687994538957	23.55447413382496	26.43679901276953	21.232038858866556
30	28.283697989253128	23.59560371482978	26.57478649717362	21.545911798743468
31	25.584595600927663	24.680912416817115	27.235752423123653	22.498739559131568
32	24.102559698720547	25.80271797227851	27.58505254605289	22.509669782948052
33	23.60086167225494	25.30413856239459	28.02519179303655	23.069807972313917
34	23.36255868780667	25.133405603882814	28.3328199668598	23.171215741450716
35	23.322461112772114	25.240982810170337	28.25901270775994	23.177543369297613
36	23.081574346953023	25.359912082130805	28.338786015972588	23.219727554943585
37	23.5194612597388	25.250135271877454	28.22241039382176	23.00799307456198
38	23.63952799813365	25.229080843505937	28.170787496637505	22.960603661722907
39	23.613983967146957	25.129028994622043	28.188813703110867	23.068173335120136
40	23.36627240272157	25.332296506313277	28.018608046919663	23.282823044045486
41	23.462399615762333	25.637514155242474	27.911430083817717	22.988656145177476
42	23.495702523751767	25.489462729405698	28.00844617791316	23.006388568929374
43	23.612484921978467	25.30158491258495	28.19477221933336	22.891157946103224
44	23.52903556330238	25.19299829899804	28.028099488690007	23.249866649009572
45	23.430309503110216	24.945595583074713	28.109484835425558	23.514610078389516
46	23.50715251699853	25.128057251774127	27.954299762480435	23.410490468746907
47	23.540862201063838	25.170331832103628	27.878775004393557	23.410030962438974
48	23.536048684166023	25.109571538992835	27.98354244260145	23.370837334239692
49	23.289436921723553	25.585235896602644	27.8028585360114	23.322468645662408
50	23.61370525020608	25.364107902024518	27.821389446134447	23.200797401634954
51	23.4522754112073	25.24327280881969	27.82025951259036	23.484192267382653
52	23.769169040092073	25.315972733046344	27.525836495452356	23.389021731409223
53	23.954244621723483	25.407037843809587	27.21904447245173	23.419673062015196
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2207.0
1	1413.5
2	620.0
3	545.5
4	471.0
5	483.5
6	496.0
7	561.5
8	791.0
9	955.0
10	1455.5
11	1956.0
12	3162.5
13	4369.0
14	5630.0
15	6891.0
16	10333.5
17	13776.0
18	19340.5
19	24905.0
20	35191.5
21	45478.0
22	193691.5
23	341905.0
24	248046.5
25	157640.0
26	161092.0
27	176815.0
28	192538.0
29	212076.5
30	231615.0
31	251249.5
32	270884.0
33	301288.5
34	331693.0
35	372041.0
36	412389.0
37	456427.0
38	500465.0
39	541243.0
40	582021.0
41	628858.5
42	722364.5
43	769033.0
44	815910.5
45	862788.0
46	898301.5
47	933815.0
48	966838.0
49	999861.0
50	996944.5
51	994028.0
52	974417.0
53	954806.0
54	924357.0
55	893908.0
56	842964.0
57	792020.0
58	713888.0
59	560106.5
60	484457.0
61	424524.5
62	364592.0
63	313285.5
64	261979.0
65	214604.5
66	167230.0
67	133913.0
68	100596.0
69	77730.0
70	54864.0
71	39720.0
72	24576.0
73	18315.0
74	12054.0
75	9175.5
76	4574.5
77	2852.0
78	2033.0
79	1214.0
80	938.0
81	662.0
82	479.5
83	297.0
84	198.0
85	99.0
86	69.0
87	39.0
88	34.5
89	30.0
90	21.0
91	12.0
92	9.5
93	5.0
94	3.0
95	3.0
96	3.0
97	2.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.3275117E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.062880973445004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.39619537458285	37.74550754888345
2	12.652145998378506	12.668057583509432
3	4.291298497454232	6.445042976987237
4	1.9970465017020365	3.9991160525257508
5	1.1341303384711514	2.838891607162707
6	0.7802271282451454	2.3436250712153712
7	0.5667146695909229	1.985995833474491
8	0.4585411103233234	1.836471122203828
9	0.36391264357590364	1.639666382406496
>10	2.246480530706995	19.707290363450863
>50	0.08182027434120995	2.7082385445521036
>100	0.02960402913677578	2.8880978230764067
>500	0.0013394533553263367	0.41827726938255566
>1k	4.226875837344527E-4	0.43330711828585616
>5k	4.528595696233605E-5	0.16811639398742806
>10k+	7.547659493722676E-5	2.174298308895931
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	232479	1.7512388026410615	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	22947	0.17285723357466454	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.039468352708304E-5	0.0	0.0	0.0	0.0
2	9.039468352708304E-5	0.0	0.0	0.0	0.0
3	9.039468352708304E-5	0.0	0.0	0.0	0.0
4	9.039468352708304E-5	0.0	0.0	7.532890293923587E-6	7.532890293923587E-6
5	1.0546046411493021E-4	0.0	0.0	3.7664451469617933E-5	1.5065780587847173E-5
6	1.0546046411493021E-4	0.0	0.0	5.273023205746511E-5	2.259867088177076E-5
7	1.0546046411493021E-4	0.0	0.0	5.273023205746511E-5	2.259867088177076E-5
8	1.0546046411493021E-4	0.0	0.0	6.779601264531227E-5	3.0131561175694347E-5
9	1.129933544088538E-4	0.0	0.0	6.779601264531227E-5	3.7664451469617933E-5
10	1.129933544088538E-4	0.0	0.0	6.779601264531227E-5	3.7664451469617933E-5
11	1.129933544088538E-4	0.0	0.0	6.779601264531227E-5	4.519734176354152E-5
12	1.2052624470277739E-4	0.0	0.0	6.779601264531227E-5	5.273023205746511E-5
13	1.2052624470277739E-4	0.0	0.0	7.532890293923587E-5	5.273023205746511E-5
14	1.2052624470277739E-4	0.0	0.0	7.532890293923587E-5	7.532890293923587E-5
15	1.2805913499670097E-4	0.0	0.0	7.532890293923587E-5	8.286179323315945E-5
16	1.2805913499670097E-4	0.0	0.0	7.532890293923587E-5	8.286179323315945E-5
17	1.5065780587847173E-4	0.0	0.0	8.286179323315945E-5	9.792757382100662E-5
18	1.5065780587847173E-4	0.0	0.0	8.286179323315945E-5	9.792757382100662E-5
19	1.5065780587847173E-4	0.0	0.0	9.039468352708304E-5	9.792757382100662E-5
20	1.5065780587847173E-4	0.0	0.0	9.039468352708304E-5	9.792757382100662E-5
21	1.732564767602425E-4	7.532890293923587E-6	0.0	9.039468352708304E-5	9.792757382100662E-5
22	1.8078936705416608E-4	7.532890293923587E-6	0.0	9.039468352708304E-5	1.0546046411493021E-4
23	1.8832225734808966E-4	7.532890293923587E-6	0.0	1.2052624470277739E-4	1.0546046411493021E-4
24	2.0338803793593685E-4	7.532890293923587E-6	0.0	1.2805913499670097E-4	1.0546046411493021E-4
25	2.1092092822986043E-4	7.532890293923587E-6	0.0	1.2805913499670097E-4	1.0546046411493021E-4
26	2.18453818523784E-4	7.532890293923587E-6	0.0	1.2805913499670097E-4	1.2052624470277739E-4
27	2.259867088177076E-4	7.532890293923587E-6	0.0	1.2805913499670097E-4	1.2805913499670097E-4
28	2.4105248940555477E-4	7.532890293923587E-6	0.0	1.581906961723953E-4	1.2805913499670097E-4
29	2.5611826999340193E-4	7.532890293923587E-6	0.0	1.581906961723953E-4	1.2805913499670097E-4
30	2.711840505812491E-4	7.532890293923587E-6	0.0	1.581906961723953E-4	1.2805913499670097E-4
31	2.937827214630199E-4	7.532890293923587E-6	0.0	1.732564767602425E-4	1.2805913499670097E-4
32	2.937827214630199E-4	7.532890293923587E-6	0.0	1.8832225734808966E-4	1.2805913499670097E-4
33	3.3898006322656137E-4	7.532890293923587E-6	0.0	1.9585514764201324E-4	1.3559202529062455E-4
34	3.766445146961793E-4	7.532890293923587E-6	0.0	2.0338803793593685E-4	1.4312491558454815E-4
35	4.2184185645972085E-4	7.532890293923587E-6	0.0	2.1092092822986043E-4	1.5065780587847173E-4
36	4.5950630792933875E-4	7.532890293923587E-6	0.0	2.1092092822986043E-4	1.5065780587847173E-4
37	4.8210497881110955E-4	7.532890293923587E-6	0.0	2.1092092822986043E-4	1.581906961723953E-4
38	5.122365399868039E-4	7.532890293923587E-6	0.0	2.18453818523784E-4	1.657235864663189E-4
39	5.348352108685747E-4	7.532890293923587E-6	0.0	2.259867088177076E-4	1.657235864663189E-4
40	5.724996623381926E-4	7.532890293923587E-6	0.0	2.259867088177076E-4	1.732564767602425E-4
41	5.950983332199633E-4	7.532890293923587E-6	0.0	2.259867088177076E-4	1.732564767602425E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	132575	0.0	44.587517	1
AGCAGTG	140075	0.0	42.431698	2
AGTGGTA	141120	0.0	41.944305	5
CAACGCA	141260	0.0	41.852825	13
TCAACGC	140710	0.0	41.82101	12
GGTATCA	141375	0.0	41.817116	8
GTGGTAT	140900	0.0	41.814655	6
GTATCAA	141600	0.0	41.777225	9
AACGCAG	141735	0.0	41.717537	14
TGGTATC	141355	0.0	41.673412	7
TATCAAC	142165	0.0	41.66574	10
ACGCAGA	141860	0.0	41.647644	15
GCAGTGG	142520	0.0	41.624615	3
ATCAACG	142015	0.0	41.54593	11
CAGTGGT	143215	0.0	41.345497	4
CGCAGAG	142905	0.0	41.339806	16
AGAGTAC	141755	0.0	41.282284	19
CAGAGTA	143105	0.0	40.96017	18
GCAGAGT	144155	0.0	40.674862	17
ACATGGG	100250	0.0	40.23012	24
>>END_MODULE