FastQCFastQC Report
Thu 26 May 2016
SRR805185_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805185_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14573248
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7021724.818225834076247No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC840250.5765701647292354No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG425560.29201451865774875No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA312870.21468789936189928No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG305800.20983654433109214No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG278000.19076049484644741No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC206600.14176661235710805No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGCTGGTGAGATGGCTCAGT159030.10912460969579327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3660800.045.5338171
AGCAGTG3720350.044.900992
AGTGGTA3747500.044.534935
GTGGTAT3751200.044.4941336
TGGTATC3756400.044.4037677
GTATCAA3753650.044.3930979
GGTATCA3758000.044.357348
GCAGTGG3770200.044.3353543
TATCAAC3761500.044.31357210
TCAACGC3766300.044.30264712
ACGCAGA3755200.044.2827815
CAGTGGT3769500.044.2700234
CAACGCA3757200.044.26671613
AACGCAG3759750.044.25856814
ATCAACG3776250.044.21391711
CGCAGAG3767150.044.1504216
AGAGTAC3667800.044.14370719
GAGTACT1152450.044.035120
CAGAGTA3701800.044.0067918
GCAGAGT3769650.043.86741317