##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805185_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14573248
Sequences flagged as poor quality	0
Sequence length	53
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.73833876977871	35.0	35.0	35.0	33.0	37.0
2	35.601190551344494	37.0	35.0	37.0	35.0	37.0
3	37.94713299327645	39.0	38.0	39.0	35.0	39.0
4	37.960502833685396	39.0	38.0	39.0	35.0	39.0
5	37.943615383475255	39.0	38.0	39.0	35.0	39.0
6	38.024817528666226	39.0	39.0	39.0	35.0	39.0
7	37.85315325725603	39.0	38.0	39.0	35.0	39.0
8	38.880850240111194	40.0	39.0	40.0	36.0	40.0
9	38.931618812772555	40.0	40.0	40.0	37.0	40.0
10	38.384340745453585	40.0	38.0	40.0	36.0	40.0
11	38.7278556571603	40.0	39.0	40.0	36.0	40.0
12	38.889459782747124	40.0	39.0	40.0	36.0	40.0
13	38.98736026450658	40.0	40.0	40.0	37.0	40.0
14	38.94791188621782	40.0	40.0	40.0	37.0	40.0
15	38.95612007700686	40.0	40.0	40.0	37.0	40.0
16	38.93302865634346	40.0	40.0	40.0	37.0	40.0
17	38.900647268199926	40.0	40.0	40.0	37.0	40.0
18	38.84577137505654	40.0	40.0	40.0	37.0	40.0
19	38.73641593143821	40.0	39.0	40.0	36.0	40.0
20	38.748523321637016	40.0	39.0	40.0	36.0	40.0
21	38.569711432894024	40.0	39.0	40.0	36.0	40.0
22	38.68946373519479	40.0	39.0	40.0	36.0	40.0
23	37.943947224393625	40.0	38.0	40.0	34.0	40.0
24	37.90625500917846	40.0	38.0	40.0	34.0	40.0
25	38.23826418105284	40.0	38.0	40.0	35.0	40.0
26	38.38126058103177	40.0	38.0	40.0	35.0	40.0
27	38.35248422314641	40.0	39.0	40.0	35.0	40.0
28	38.53548982354517	40.0	39.0	40.0	35.0	40.0
29	38.572564949145175	40.0	39.0	40.0	36.0	40.0
30	38.56531872647745	40.0	39.0	40.0	35.0	40.0
31	38.322261036112195	40.0	39.0	40.0	35.0	40.0
32	38.18792543707484	40.0	39.0	40.0	35.0	40.0
33	38.16319477991454	40.0	38.0	40.0	35.0	40.0
34	38.149396826294314	40.0	38.0	40.0	35.0	40.0
35	38.11387324225869	40.0	38.0	40.0	35.0	40.0
36	38.000626833496554	40.0	38.0	40.0	34.0	40.0
37	37.75373252414287	40.0	37.0	40.0	34.0	40.0
38	37.71726220537796	40.0	37.0	40.0	34.0	40.0
39	37.668367751650145	40.0	37.0	40.0	34.0	40.0
40	37.615276361179056	40.0	37.0	40.0	33.0	40.0
41	37.54750814643379	40.0	36.0	40.0	33.0	40.0
42	37.454348955016755	40.0	36.0	40.0	33.0	40.0
43	37.18479648462718	40.0	36.0	40.0	33.0	40.0
44	37.14374283618861	40.0	35.0	40.0	33.0	40.0
45	37.25788129043025	40.0	35.0	40.0	33.0	40.0
46	37.25961817159771	40.0	35.0	40.0	33.0	40.0
47	37.203943486036884	40.0	35.0	40.0	33.0	40.0
48	37.135404749853976	40.0	35.0	40.0	33.0	40.0
49	36.89943882105074	39.0	35.0	40.0	33.0	40.0
50	36.81082439549509	39.0	35.0	40.0	32.0	40.0
51	36.28585254296091	39.0	35.0	40.0	31.0	40.0
52	36.08347150889081	39.0	35.0	40.0	30.0	40.0
53	35.71289728960902	38.0	35.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	5.0
12	9.0
13	45.0
14	147.0
15	350.0
16	830.0
17	1471.0
18	2611.0
19	4459.0
20	7070.0
21	10870.0
22	15636.0
23	21412.0
24	29250.0
25	39501.0
26	51895.0
27	65889.0
28	82538.0
29	101792.0
30	124405.0
31	154171.0
32	194528.0
33	253280.0
34	351737.0
35	528888.0
36	873918.0
37	1744203.0
38	2745949.0
39	7166388.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.36588796128358	47.74891637059906	29.05210972873034	9.833085939387018
2	23.48425690690229	52.27822239764258	10.403617642408886	13.83390305304624
3	47.443438827089196	22.11852155401459	15.685638506940938	14.752401111955276
4	20.89453222781908	19.67905850500863	16.205508888615633	43.22090037855666
5	17.921433849200945	45.11635292283505	19.149423656277584	17.812789571686423
6	42.99638625514367	20.592945374977493	17.60722112188031	18.803447247998527
7	19.598746964300613	19.382995472251622	43.31289085315779	17.705366710289976
8	41.939003576965135	21.316044302546693	19.335401414976264	17.409550705511908
9	42.53624861115381	20.698714521292715	19.207619330982357	17.557417536571123
10	18.243771052273317	20.8588984418573	43.53186743270958	17.365463073159805
11	17.603248088552395	44.7824054047526	20.053072588897138	17.56127391779787
12	17.596317581365525	20.987037344043003	43.950435757354846	17.466209317236626
13	17.397055206910636	20.895396825745365	20.249693136355052	41.45785483098894
14	17.69004411370753	44.930004622167964	19.749193865362066	17.630757398762444
15	17.672312994330433	44.85714509215791	19.89541384322836	17.575128070283302
16	17.555365831968274	20.65271928399215	20.103912319340207	41.688002564699374
17	41.70306440952628	20.433962284866077	20.12097783555183	17.74199547005582
18	18.004730311321126	20.285354369870053	20.18390135129794	41.52601396751088
19	17.800177420983985	44.62089027785707	20.018275953308418	17.56065634785053
20	42.09184527704462	20.70629004598014	19.986848504876882	17.215016172098355
21	18.19085903156249	44.287038826210875	20.131366734443827	17.39073540778281
22	41.77978032076309	20.668762378846502	20.14659326459002	17.404864035800394
23	18.10779587364464	20.41067303596288	43.78872163569851	17.69280945469397
24	17.89074062281792	43.97711477908013	20.138516822056417	17.993627776045532
25	17.919443901592835	20.659738995726965	20.074289547532576	41.346527555147624
26	18.117320174610356	36.62362021149987	27.44923437795061	17.809825235939165
27	18.53142140996983	20.353321373519478	43.682280024329515	17.43297719218118
28	34.71032675763151	20.138798159476874	27.78303779637868	17.36783728651293
29	34.862791053854295	20.253082909177145	27.47046506036266	17.4136609766059
30	34.55091823044526	20.147670581053724	27.601705536061694	17.699705652439317
31	27.944628404045552	22.79985216747838	29.24140177947977	20.014117648996297
32	23.8052354560905	25.608951415635005	30.292828338610583	20.292984789663908
33	23.219882074332364	24.46508835916331	31.092382425661047	21.222647140843275
34	22.365178990984028	23.653093668618006	32.12701108222409	21.85471625817388
35	21.735408606235207	24.331905969074295	31.996196043599888	21.936489381090613
36	21.796959744320553	24.465959818977897	32.018353080932954	21.718727355768596
37	22.68829844932303	23.849611287751365	32.23291746630538	21.22917279662022
38	22.82615378534696	23.969488476419258	32.0628867360248	21.141471002208977
39	23.267229103628786	23.831482178852646	31.68973725006258	21.211551467455987
40	22.0792921385816	24.471943385578836	31.432183134466662	22.016581341372905
41	22.463756878356836	25.29591207121432	31.070136183780033	21.17019486664881
42	22.626191498285078	25.140874566877606	31.15794776840413	21.07498616643318
43	22.907796532385916	24.26483787279267	32.07195815236247	20.75540744245895
44	22.455097175317405	24.148405352053295	31.440410538542952	21.956086934086347
45	22.503521521077523	23.581345764513166	31.548032394700208	22.3671003197091
46	22.46112877513647	23.73940936159187	31.230237761684972	22.569224101586688
47	22.42614686856355	23.948209760789084	31.17064226176622	22.455001108881152
48	22.67926134242689	23.841864215856344	31.30567736169727	22.1731970800195
49	21.87656451053327	25.117022643133502	30.989831504960325	22.016581341372905
50	22.65908052892533	24.47501751153895	31.044939329928372	21.820962629607347
51	22.73162441207341	24.311999631104886	30.75797516106224	22.19840079575946
52	22.93691838634737	24.249007496475734	30.470414007913675	22.34366010926322
53	23.31201664858788	24.586543782141085	29.624151047179048	22.47728852209199
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3217.0
1	2049.0
2	881.0
3	712.0
4	543.0
5	570.0
6	597.0
7	669.0
8	949.5
9	1158.0
10	1765.5
11	2373.0
12	3600.0
13	4827.0
14	6017.0
15	7207.0
16	10545.0
17	13883.0
18	19844.5
19	25806.0
20	38505.0
21	51204.0
22	468536.5
23	885869.0
24	575308.0
25	238357.5
26	211968.0
27	216901.0
28	221834.0
29	237249.5
30	252665.0
31	268438.0
32	284211.0
33	314767.5
34	345324.0
35	385752.5
36	426181.0
37	469248.0
38	512315.0
39	557903.5
40	603492.0
41	653727.0
42	754380.0
43	804798.0
44	867655.0
45	930512.0
46	969437.5
47	1008363.0
48	1075315.5
49	1142268.0
50	1108597.0
51	1074926.0
52	1040890.5
53	1006855.0
54	974238.0
55	941621.0
56	912371.0
57	883121.0
58	762008.0
59	550209.5
60	459524.0
61	393596.0
62	327668.0
63	276936.5
64	226205.0
65	183048.5
66	139892.0
67	111308.0
68	82724.0
69	62838.5
70	42953.0
71	30830.0
72	18707.0
73	13886.5
74	9066.0
75	6807.0
76	3213.5
77	1879.0
78	1369.5
79	860.0
80	654.5
81	449.0
82	327.5
83	206.0
84	143.5
85	81.0
86	60.5
87	40.0
88	38.5
89	37.0
90	28.0
91	19.0
92	17.0
93	10.0
94	5.0
95	4.5
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.4573248E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.48950867071349
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.50793093089563	32.40313308256107
2	13.384092074898257	11.641351766818309
3	4.282332501327351	5.587096093420621
4	2.0271845365589245	3.526450379192626
5	1.1619166737358035	2.5265592628539904
6	0.7396426435086754	1.9300017094859991
7	0.5278510885169619	1.6069189143632776
8	0.38618297500437704	1.343592627194783
9	0.31286649378649717	1.2245769084873237
>10	2.502179164205383	20.929800133076974
>50	0.11232859647629921	3.2662202879283635
>100	0.04948642972403838	3.926787855200759
>500	0.003679108668556749	1.1076776240233168
>1k	0.0020102261943484253	1.677091985388426
>5k	1.266225996098427E-4	0.3995907672724709
>10k+	1.8993389941476403E-4	6.903150602731599
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	702172	4.818225834076247	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	84025	0.5765701647292354	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	42556	0.29201451865774875	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA	31287	0.21468789936189928	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	30580	0.20983654433109214	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	27800	0.19076049484644741	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC	20660	0.14176661235710805	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGCTGGTGAGATGGCTCAGT	15903	0.10912460969579327	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0292832455743566E-4	0.0	0.0	0.0	1.3723776607658087E-5
2	9.606643625360661E-4	0.0	0.0	6.861888303829044E-6	2.7447553215316174E-5
3	9.743881391437242E-4	0.0	0.0	6.861888303829044E-6	2.7447553215316174E-5
4	0.001104764016916476	0.0	0.0	6.861888303829044E-6	3.430944151914522E-5
5	0.001104764016916476	0.0	0.0	6.861888303829044E-6	4.803321812680331E-5
6	0.001118487793524134	0.0	0.0	6.861888303829044E-6	5.489510643063235E-5
7	0.0011322115701317922	0.0	0.0	6.861888303829044E-6	5.489510643063235E-5
8	0.0011596591233471083	0.0	0.0	6.861888303829044E-6	5.489510643063235E-5
9	0.0011596591233471083	0.0	0.0	6.861888303829044E-6	6.17569947344614E-5
10	0.0011596591233471083	0.0	0.0	6.861888303829044E-6	6.861888303829044E-5
11	0.0011596591233471083	0.0	0.0	6.861888303829044E-6	8.234265964594852E-5
12	0.0011596591233471083	0.0	0.0	1.3723776607658087E-5	8.234265964594852E-5
13	0.0011733828999547665	6.861888303829044E-6	0.0	2.058566491148713E-5	8.920454794977756E-5
14	0.0011939685648662535	6.861888303829044E-6	0.0	2.058566491148713E-5	8.920454794977756E-5
15	0.0012008304531700826	6.861888303829044E-6	0.0	2.058566491148713E-5	1.0292832455743566E-4
16	0.0012282780063853987	6.861888303829044E-6	0.0	2.058566491148713E-5	1.1665210116509374E-4
17	0.0012351398946892278	6.861888303829044E-6	0.0	2.058566491148713E-5	1.235139894689228E-4
18	0.001255725559600715	6.861888303829044E-6	0.0	2.7447553215316174E-5	1.3037587777275182E-4
19	0.001255725559600715	6.861888303829044E-6	0.0	2.7447553215316174E-5	1.3723776607658088E-4
20	0.001269449336208373	6.861888303829044E-6	0.0	2.7447553215316174E-5	1.440996543804099E-4
21	0.0012831731128160311	2.058566491148713E-5	0.0	4.803321812680331E-5	1.440996543804099E-4
22	0.0012900350011198602	2.058566491148713E-5	0.0	4.803321812680331E-5	1.57823430988068E-4
23	0.0013174825543351763	2.058566491148713E-5	0.0	4.803321812680331E-5	1.57823430988068E-4
24	0.0013312063309428345	2.058566491148713E-5	0.0	4.803321812680331E-5	1.6468531929189704E-4
25	0.0013517919958543215	2.7447553215316174E-5	0.0	5.489510643063235E-5	1.715472075957261E-4
26	0.0013792395490696378	2.7447553215316174E-5	0.0	6.17569947344614E-5	1.9213287250721323E-4
27	0.001413548990588783	2.7447553215316174E-5	0.0	6.17569947344614E-5	1.9213287250721323E-4
28	0.0014204108788926119	2.7447553215316174E-5	0.0	6.17569947344614E-5	2.1271853741870034E-4
29	0.00143413465550027	2.7447553215316174E-5	0.0	6.17569947344614E-5	2.2644231402635842E-4
30	0.0014615822087155862	2.7447553215316174E-5	0.0	6.17569947344614E-5	2.538898672416746E-4
31	0.0014821678736270734	2.7447553215316174E-5	0.0	6.17569947344614E-5	2.6075175554550364E-4
32	0.0015027535385385604	2.7447553215316174E-5	0.0	6.17569947344614E-5	2.7447553215316175E-4
33	0.0015164773151462186	2.7447553215316174E-5	0.0	6.17569947344614E-5	2.7447553215316175E-4
34	0.0015233392034500477	2.7447553215316174E-5	0.0	6.17569947344614E-5	2.7447553215316175E-4
35	0.001564510533273022	2.7447553215316174E-5	0.0	6.861888303829044E-5	2.881993087608198E-4
36	0.0016056818630959962	2.7447553215316174E-5	0.0	8.234265964594852E-5	2.881993087608198E-4
37	0.0016331294163113123	2.7447553215316174E-5	0.0	8.234265964594852E-5	2.881993087608198E-4
38	0.0016605769695266284	2.7447553215316174E-5	0.0	8.234265964594852E-5	2.881993087608198E-4
39	0.0016674388578304575	2.7447553215316174E-5	0.0	8.234265964594852E-5	2.881993087608198E-4
40	0.00172233396426109	2.7447553215316174E-5	0.0	8.234265964594852E-5	3.019230853684779E-4
41	0.0018115385122108674	2.7447553215316174E-5	0.0	8.920454794977756E-5	3.0878497367230694E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	366080	0.0	45.533817	1
AGCAGTG	372035	0.0	44.90099	2
AGTGGTA	374750	0.0	44.53493	5
GTGGTAT	375120	0.0	44.494133	6
TGGTATC	375640	0.0	44.403767	7
GTATCAA	375365	0.0	44.393097	9
GGTATCA	375800	0.0	44.35734	8
GCAGTGG	377020	0.0	44.335354	3
TATCAAC	376150	0.0	44.313572	10
TCAACGC	376630	0.0	44.302647	12
ACGCAGA	375520	0.0	44.28278	15
CAGTGGT	376950	0.0	44.270023	4
CAACGCA	375720	0.0	44.266716	13
AACGCAG	375975	0.0	44.258568	14
ATCAACG	377625	0.0	44.213917	11
CGCAGAG	376715	0.0	44.15042	16
AGAGTAC	366780	0.0	44.143707	19
GAGTACT	115245	0.0	44.0351	20
CAGAGTA	370180	0.0	44.00679	18
GCAGAGT	376965	0.0	43.867413	17
>>END_MODULE