FastQCFastQC Report
Thu 26 May 2016
SRR805184_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805184_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13601829
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5180603.808752484684229No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC500840.36821518635471745No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG439290.32296391904353455No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG356060.26177361882729155No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA231020.1698448054302109No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG195310.14359098324203312No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC151880.11166145376478413No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGCTGGTGAGATGGCTCAGT147380.1083530751636416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2762800.045.644161
AGCAGTG2820000.044.7916682
GTGGTAT2833500.044.5392846
AGTGGTA2835750.044.5014535
GTATCAA2836950.044.459449
TCAACGC2838300.044.45153812
GGTATCA2838350.044.4275748
CAACGCA2830550.044.4196513
TGGTATC2840900.044.404247
ACGCAGA2835200.044.3675215
AACGCAG2836700.044.3415814
ATCAACG2847000.044.3412911
TATCAAC2844650.044.3374410
GCAGTGG2853800.044.2998623
AGAGTAC2774500.044.1879619
CGCAGAG2847050.044.18285816
CAGTGGT2860850.044.1143044
CAGAGTA2808000.043.9637518
GCAGAGT2859450.043.8088117
GAGTACT836300.043.74883320