##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805184_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13601829
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.269375243579375	37.0	37.0	37.0	35.0	37.0
2	36.28195568404808	37.0	37.0	37.0	35.0	37.0
3	38.14938064579403	39.0	39.0	39.0	37.0	39.0
4	38.14138120689504	39.0	39.0	39.0	37.0	39.0
5	38.09357785633094	39.0	39.0	39.0	37.0	39.0
6	38.13395639659931	39.0	39.0	39.0	37.0	39.0
7	37.978753960221084	39.0	38.0	39.0	35.0	39.0
8	38.99439171011487	40.0	39.0	40.0	37.0	40.0
9	39.02872878345993	40.0	40.0	40.0	37.0	40.0
10	38.61580806522417	40.0	38.0	40.0	36.0	40.0
11	38.88153769614366	40.0	39.0	40.0	37.0	40.0
12	39.008413868458426	40.0	40.0	40.0	37.0	40.0
13	39.09710848445455	40.0	40.0	40.0	37.0	40.0
14	39.066559210529704	40.0	40.0	40.0	37.0	40.0
15	39.09170656387461	40.0	40.0	40.0	37.0	40.0
16	39.06223060148749	40.0	40.0	40.0	37.0	40.0
17	39.03984721466503	40.0	40.0	40.0	37.0	40.0
18	38.98531116660855	40.0	40.0	40.0	37.0	40.0
19	38.865727395925944	40.0	40.0	40.0	37.0	40.0
20	38.892695019177204	40.0	40.0	40.0	37.0	40.0
21	38.756698896891	40.0	40.0	40.0	36.0	40.0
22	38.85230603913635	40.0	40.0	40.0	37.0	40.0
23	38.2721070085501	40.0	38.0	40.0	35.0	40.0
24	38.14697427823861	40.0	38.0	40.0	35.0	40.0
25	38.44208076722623	40.0	39.0	40.0	35.0	40.0
26	38.579161964174084	40.0	39.0	40.0	36.0	40.0
27	38.519017699752	40.0	39.0	40.0	36.0	40.0
28	38.6808762262781	40.0	39.0	40.0	36.0	40.0
29	38.714609336729644	40.0	39.0	40.0	36.0	40.0
30	38.70611937556339	40.0	40.0	40.0	36.0	40.0
31	38.492410836807316	40.0	39.0	40.0	35.0	40.0
32	38.38768543553959	40.0	39.0	40.0	35.0	40.0
33	38.35274175259813	40.0	39.0	40.0	35.0	40.0
34	38.33563875858166	40.0	39.0	40.0	35.0	40.0
35	38.29201447834699	40.0	39.0	40.0	35.0	40.0
36	38.21070791288436	40.0	38.0	40.0	35.0	40.0
37	38.119174046372734	40.0	38.0	40.0	35.0	40.0
38	37.98018825262397	40.0	38.0	40.0	35.0	40.0
39	37.92235801523457	40.0	38.0	40.0	34.0	40.0
40	37.87712453964831	40.0	37.0	40.0	34.0	40.0
41	37.82264642497711	40.0	37.0	40.0	34.0	40.0
42	37.730663501210024	40.0	37.0	40.0	34.0	40.0
43	37.44902159849238	40.0	37.0	40.0	33.0	40.0
44	37.45100721380926	40.0	36.0	40.0	33.0	40.0
45	37.57446097874043	40.0	37.0	40.0	33.0	40.0
46	37.58113449301561	40.0	37.0	40.0	34.0	40.0
47	37.52294636258109	40.0	36.0	40.0	34.0	40.0
48	37.46225775959983	40.0	36.0	40.0	33.0	40.0
49	37.247862695524255	40.0	36.0	40.0	33.0	40.0
50	37.17278051356182	40.0	35.0	40.0	33.0	40.0
51	36.66375110288477	39.0	35.0	40.0	32.0	40.0
52	36.536576808898275	39.0	35.0	40.0	31.0	40.0
53	36.22638918633663	39.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	4.0
12	16.0
13	53.0
14	128.0
15	312.0
16	662.0
17	1327.0
18	2181.0
19	3591.0
20	5490.0
21	8248.0
22	11832.0
23	16066.0
24	22172.0
25	29873.0
26	38847.0
27	50434.0
28	63456.0
29	79358.0
30	97923.0
31	122943.0
32	155902.0
33	203279.0
34	280691.0
35	424321.0
36	702145.0
37	1382458.0
38	2397092.0
39	7501021.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.873812779149038	42.948687268454854	26.85857909256174	17.318920859834364
2	23.668919819533095	48.04342857126053	11.974036726972527	16.31361488223385
3	42.679738144039305	23.0677727238006	16.42538661528534	17.827102516874753
4	20.552860942451197	20.597994578523227	17.882440662943196	40.966703816082386
5	18.913191747962717	41.04076003308085	20.644113376223153	19.401934842733283
6	39.53284517839476	21.149023414424633	19.135735348532908	20.1823960586477
7	20.469798583705177	20.339095573102707	39.99369496558147	19.19741087761065
8	38.97610387544205	22.065084041271216	20.308026222061752	18.650785861224985
9	39.00934940440731	21.563747051958966	20.56637383104875	18.860529712584974
10	19.108511068621727	21.700287512804344	40.34562557726611	18.845575841307813
11	18.807911788921917	41.338933168473154	20.926972394668393	18.92618264793654
12	18.6924273198847	21.755000742914795	40.58246872534569	18.970103211854816
13	18.67900265471651	21.74357580881218	21.01515171231751	38.562269824153795
14	19.115848317163817	41.45147685653157	20.616403867450472	18.816270958854137
15	18.887077612871035	41.30328355105773	20.723095401361096	19.08654343471014
16	18.996658464093322	21.371214121277365	20.92712678566978	38.70500062895953
17	38.777204153941355	21.258596913694475	20.906820693011213	19.057378239352957
18	19.087991769342196	21.329124193518386	20.927413511815214	38.655470525324205
19	19.253410699399325	41.05711812727538	20.827353439011766	18.862117734313525
20	38.99483665027696	21.487683751942477	20.896851445493102	18.62062815228746
21	19.31944593627813	40.88368556905104	20.99210334139622	18.80476515327461
22	38.633473483602835	21.305414146876863	21.152405312550247	18.90870705697006
23	19.211982447360572	21.380551100885032	40.4624186938389	18.945047757915496
24	19.125339687772872	40.60476719711738	20.954946573729167	19.314946541380575
25	19.160820210282015	21.419722303522562	21.00038899180397	38.41906849439145
26	19.33030477004232	34.803532671966394	26.8500287718659	19.01613378612538
27	19.73844105818416	21.189157722832714	40.28252376941366	18.789877449569467
28	33.101018987961105	21.037648686805284	26.975644231375064	18.885688093858555
29	33.236316968842935	21.196142077657353	26.73027281845699	18.83726813504272
30	32.94590749523465	21.128915824482135	26.840125691919813	19.085050988363403
31	27.808326365520404	23.09526167399987	28.14210500661345	20.95430695386628
32	24.41880426522051	25.463193222029183	28.889467732611546	21.22853478013876
33	23.86635650249683	24.380177107064057	29.709982385457135	22.043484004981977
34	23.018286731879954	23.877862308076363	30.46138868530107	22.642462274742613
35	22.472036665069087	24.25243693329772	30.553707152177843	22.72181924945535
36	22.49267359558777	24.465040694159587	30.662251378105104	22.380034332147535
37	23.47395339259154	24.002227935669534	30.540635380727103	21.98318329101182
38	23.597142707793196	24.124505608767763	30.43015759130629	21.848194092132754
39	23.640548635040183	24.06016867290421	30.304115718555202	21.995166973500403
40	22.803139195471434	24.631363914367693	30.019029058518527	22.546467831642346
41	23.1601426543445	25.29249559011512	29.620854665942353	21.926507089598026
42	23.2163189229919	25.291253110151583	29.781913888198417	21.710514078658097
43	23.4438250914638	24.49975661361424	30.484944340941205	21.571473953980746
44	23.21726732485756	24.25672312157431	30.08463053020296	22.441379023365165
45	23.297771204152028	23.836184089654413	30.00900099538084	22.857043710812714
46	23.141343711937566	23.954072647141793	29.733493929382586	23.17108971153806
47	23.02422710945712	24.169264295265	29.801323042658456	23.00518555261943
48	22.94855346292032	24.040215473963098	30.028924786512167	22.98230627660442
49	22.43762217566476	25.068981531821933	29.635551218883872	22.857845073629438
50	23.342110829359786	24.72016079602236	29.47177912617487	22.465949248442985
51	23.39413324487464	24.45937969077541	29.46191280599102	22.684574258358932
52	23.449611078039577	24.370288731022864	29.31794687317419	22.86215331776337
53	23.978944302269937	24.6452296966827	28.476368876568	22.899457124479362
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5069.0
1	3052.0
2	1035.0
3	810.0
4	585.0
5	570.5
6	556.0
7	624.0
8	842.5
9	993.0
10	1582.5
11	2172.0
12	3372.0
13	4572.0
14	5525.5
15	6479.0
16	9457.5
17	12436.0
18	17346.5
19	22257.0
20	33697.5
21	45138.0
22	352156.5
23	659175.0
24	433487.5
25	191264.0
26	174728.0
27	181200.5
28	187673.0
29	203804.5
30	219936.0
31	235376.5
32	250817.0
33	278745.5
34	306674.0
35	344160.0
36	381646.0
37	420883.0
38	460120.0
39	503143.0
40	546166.0
41	593133.5
42	689157.5
43	738214.0
44	795854.5
45	853495.0
46	892359.5
47	931224.0
48	985302.5
49	1039381.0
50	1029278.0
51	1019175.0
52	996253.0
53	973331.0
54	947427.5
55	921524.0
56	904916.5
57	888309.0
58	774837.0
59	571149.5
60	480934.0
61	414073.5
62	347213.0
63	297708.0
64	248203.0
65	203635.5
66	159068.0
67	127520.5
68	95973.0
69	74846.5
70	53720.0
71	39891.0
72	26062.0
73	19688.0
74	13314.0
75	10262.0
76	5420.5
77	3631.0
78	2688.5
79	1746.0
80	1403.5
81	1061.0
82	788.0
83	515.0
84	342.5
85	170.0
86	114.5
87	59.0
88	54.0
89	49.0
90	40.5
91	32.0
92	22.0
93	10.5
94	9.0
95	7.0
96	5.0
97	4.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.3601829E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.322512459728294
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.72800606759739	32.678104675620084
2	13.558268711171612	12.01873067766496
3	4.577027482003794	6.085960727988959
4	2.1176798860165413	3.7544357253473652
5	1.2433918862010622	2.755512618423582
6	0.8312669107470357	2.21063028053672
7	0.5588516893419472	1.733879767479906
8	0.4205676678535946	1.4912492558879853
9	0.3208971060577432	1.2800669383381564
>10	2.4998515978134837	21.913061298022278
>50	0.1008246413548057	2.947882223882615
>100	0.03889379809252024	3.2627135700544283
>500	0.0027094547811892054	0.807445938484185
>1k	0.0014969769239228353	1.3479207882612587
>5k	1.1642926893597697E-4	0.32800479373361097
>10k+	1.496947743462561E-4	5.384400720274083
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	518060	3.808752484684229	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	50084	0.36821518635471745	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	43929	0.32296391904353455	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	35606	0.26177361882729155	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA	23102	0.1698448054302109	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	19531	0.14359098324203312	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC	15188	0.11166145376478413	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGCTGGTGAGATGGCTCAGT	14738	0.1083530751636416	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1320662096251908E-4	7.351952446983417E-6	0.0	0.0	7.351952446983417E-6
2	0.0051610706177823585	7.351952446983417E-6	0.0	0.0	7.351952446983417E-6
3	0.0051831264751233085	7.351952446983417E-6	0.0	0.0	1.4703904893966834E-5
4	0.005778634623328965	1.4703904893966834E-5	0.0	0.0	2.205585734095025E-5
5	0.0057859865757759485	1.4703904893966834E-5	0.0	0.0	2.9407809787933667E-5
6	0.005793338528222933	2.205585734095025E-5	0.0	0.0	2.9407809787933667E-5
7	0.005793338528222933	3.675976223491708E-5	0.0	0.0	2.9407809787933667E-5
8	0.005793338528222933	3.675976223491708E-5	0.0	0.0	2.9407809787933667E-5
9	0.005793338528222933	3.675976223491708E-5	0.0	0.0	2.9407809787933667E-5
10	0.005808042433116899	3.675976223491708E-5	0.0	0.0	2.9407809787933667E-5
11	0.005808042433116899	4.41117146819005E-5	0.0	7.351952446983417E-6	4.41117146819005E-5
12	0.005830098290457849	4.41117146819005E-5	0.0	7.351952446983417E-6	4.41117146819005E-5
13	0.005837450242904833	4.41117146819005E-5	0.0	1.4703904893966834E-5	4.41117146819005E-5
14	0.005837450242904833	4.41117146819005E-5	0.0	1.4703904893966834E-5	5.8815619575867335E-5
15	0.005844802195351816	4.41117146819005E-5	0.0	1.4703904893966834E-5	5.8815619575867335E-5
16	0.0058595061002457835	4.41117146819005E-5	0.0	1.4703904893966834E-5	6.616757202285075E-5
17	0.005866858052692767	4.41117146819005E-5	0.0	1.4703904893966834E-5	6.616757202285075E-5
18	0.005866858052692767	4.41117146819005E-5	0.0	2.205585734095025E-5	6.616757202285075E-5
19	0.005866858052692767	4.41117146819005E-5	0.0	2.205585734095025E-5	6.616757202285075E-5
20	0.0058962658624807	4.41117146819005E-5	0.0	2.205585734095025E-5	8.087147691681758E-5
21	0.0058962658624807	5.146366712888392E-5	0.0	2.205585734095025E-5	8.8223429363801E-5
22	0.005903617814927683	5.8815619575867335E-5	0.0	2.205585734095025E-5	9.557538181078441E-5
23	0.00591832171982165	5.8815619575867335E-5	0.0	2.205585734095025E-5	1.0292733425776784E-4
24	0.00591832171982165	5.8815619575867335E-5	0.0	2.205585734095025E-5	1.1763123915173467E-4
25	0.0059330256247156175	6.616757202285075E-5	0.0	2.205585734095025E-5	1.1763123915173467E-4
26	0.005940377577162601	6.616757202285075E-5	0.0	2.9407809787933667E-5	1.1763123915173467E-4
27	0.005947729529609584	6.616757202285075E-5	0.0	2.9407809787933667E-5	1.323351440457015E-4
28	0.005984489291844501	6.616757202285075E-5	0.0	3.675976223491708E-5	1.3968709649268493E-4
29	0.005984489291844501	6.616757202285075E-5	7.351952446983417E-6	3.675976223491708E-5	1.3968709649268493E-4
30	0.005984489291844501	7.351952446983417E-5	7.351952446983417E-6	3.675976223491708E-5	1.3968709649268493E-4
31	0.006013897101632435	7.351952446983417E-5	7.351952446983417E-6	3.675976223491708E-5	1.4703904893966833E-4
32	0.006035952958973385	7.351952446983417E-5	7.351952446983417E-6	5.146366712888392E-5	1.4703904893966833E-4
33	0.006065360768761319	7.351952446983417E-5	7.351952446983417E-6	5.146366712888392E-5	1.4703904893966833E-4
34	0.006102120530996236	7.351952446983417E-5	7.351952446983417E-6	5.8815619575867335E-5	1.4703904893966833E-4
35	0.006146232245678136	7.351952446983417E-5	7.351952446983417E-6	5.8815619575867335E-5	1.4703904893966833E-4
36	0.00617564005546607	7.351952446983417E-5	7.351952446983417E-6	5.8815619575867335E-5	1.5439100138665176E-4
37	0.0061976959128070205	7.351952446983417E-5	7.351952446983417E-6	5.8815619575867335E-5	1.6174295383363516E-4
38	0.006227103722594954	7.351952446983417E-5	7.351952446983417E-6	5.8815619575867335E-5	1.76446858727602E-4
39	0.006249159579935904	7.351952446983417E-5	7.351952446983417E-6	5.8815619575867335E-5	1.76446858727602E-4
40	0.006307975199511771	7.351952446983417E-5	7.351952446983417E-6	5.8815619575867335E-5	1.76446858727602E-4
41	0.006352086914193672	7.351952446983417E-5	7.351952446983417E-6	5.8815619575867335E-5	1.9850271606855225E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	276280	0.0	45.64416	1
AGCAGTG	282000	0.0	44.791668	2
GTGGTAT	283350	0.0	44.539284	6
AGTGGTA	283575	0.0	44.501453	5
GTATCAA	283695	0.0	44.45944	9
TCAACGC	283830	0.0	44.451538	12
GGTATCA	283835	0.0	44.427574	8
CAACGCA	283055	0.0	44.41965	13
TGGTATC	284090	0.0	44.40424	7
ACGCAGA	283520	0.0	44.36752	15
AACGCAG	283670	0.0	44.34158	14
ATCAACG	284700	0.0	44.34129	11
TATCAAC	284465	0.0	44.33744	10
GCAGTGG	285380	0.0	44.299862	3
AGAGTAC	277450	0.0	44.18796	19
CGCAGAG	284705	0.0	44.182858	16
CAGTGGT	286085	0.0	44.114304	4
CAGAGTA	280800	0.0	43.96375	18
GCAGAGT	285945	0.0	43.80881	17
GAGTACT	83630	0.0	43.748833	20
>>END_MODULE