FastQCFastQC Report
Thu 26 May 2016
SRR805183_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805183_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13621764
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT948680.69644430780037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT578350.039.0887871
AGCAGTG652400.035.181562
GCAGTGG703500.032.7964483
AGTGGTA703000.032.7863435
TCAACGC699750.032.67331312
CAACGCA700750.032.59650813
GTGGTAT707950.032.563746
GGTATCA710150.032.452938
AACGCAG705350.032.4005814
ACGCAGA706100.032.3628415
GTATCAA711950.032.3510789
TGGTATC714250.032.2534837
ATCAACG709400.032.2354811
CAGTGGT720650.031.9800874
TATCAAC715150.031.97629710
CGCAGAG718200.031.81105416
AGAGTAC714600.031.7772919
CAGAGTA731450.031.20910318
GCAGAGT747250.030.69387617
GAGTACT220200.030.58628320
Produced by FastQC (version 0.11.5)