##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805183_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13621764
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.034748216163486	37.0	37.0	37.0	35.0	37.0
2	35.93239605384442	37.0	37.0	37.0	35.0	37.0
3	38.00179594948202	39.0	39.0	39.0	35.0	39.0
4	38.056576813399495	39.0	39.0	39.0	37.0	39.0
5	38.07623740948676	39.0	39.0	39.0	37.0	39.0
6	38.05753190262289	39.0	39.0	39.0	37.0	39.0
7	38.084539711596825	39.0	39.0	39.0	37.0	39.0
8	39.032396758598956	40.0	40.0	40.0	37.0	40.0
9	39.01515361740227	40.0	40.0	40.0	37.0	40.0
10	39.011744440734695	40.0	40.0	40.0	37.0	40.0
11	39.018428376824026	40.0	40.0	40.0	37.0	40.0
12	39.0100161036412	40.0	40.0	40.0	37.0	40.0
13	39.06651091591368	40.0	40.0	40.0	37.0	40.0
14	39.015484705211456	40.0	40.0	40.0	37.0	40.0
15	39.01156810527623	40.0	40.0	40.0	37.0	40.0
16	38.86784736543667	40.0	40.0	40.0	37.0	40.0
17	38.91031528662514	40.0	40.0	40.0	37.0	40.0
18	38.89381918523915	40.0	40.0	40.0	37.0	40.0
19	38.84388806031289	40.0	40.0	40.0	37.0	40.0
20	38.81710797514918	40.0	40.0	40.0	37.0	40.0
21	38.781711384810365	40.0	40.0	40.0	37.0	40.0
22	38.78055595442705	40.0	40.0	40.0	37.0	40.0
23	38.68414311098034	40.0	39.0	40.0	36.0	40.0
24	38.63747881698728	40.0	39.0	40.0	36.0	40.0
25	38.640066293910245	40.0	39.0	40.0	36.0	40.0
26	38.608460328632916	40.0	39.0	40.0	36.0	40.0
27	38.56748266964543	40.0	39.0	40.0	36.0	40.0
28	38.514569038195056	40.0	39.0	40.0	36.0	40.0
29	38.446056178920735	40.0	39.0	40.0	35.0	40.0
30	38.39445045443453	40.0	39.0	40.0	35.0	40.0
31	38.360110188372076	40.0	39.0	40.0	35.0	40.0
32	38.30397891198233	40.0	39.0	40.0	35.0	40.0
33	38.24996483568501	40.0	38.0	40.0	35.0	40.0
34	38.21426387947993	40.0	38.0	40.0	35.0	40.0
35	38.14099062353451	40.0	38.0	40.0	35.0	40.0
36	38.054773963195956	40.0	38.0	40.0	34.0	40.0
37	38.00894803345587	40.0	38.0	40.0	34.0	40.0
38	37.40222881559246	40.0	38.0	40.0	33.0	40.0
39	37.646471778544985	40.0	38.0	40.0	33.0	40.0
40	37.511720214797435	40.0	38.0	40.0	33.0	40.0
41	37.52583850373564	40.0	37.0	40.0	33.0	40.0
42	37.49671510973175	40.0	37.0	40.0	33.0	40.0
43	37.47391622700261	40.0	37.0	40.0	33.0	40.0
44	37.53166733765172	40.0	37.0	40.0	33.0	40.0
45	37.49430205955704	40.0	37.0	40.0	33.0	40.0
46	37.50120175331183	40.0	37.0	40.0	33.0	40.0
47	37.34623078185762	40.0	37.0	40.0	33.0	40.0
48	37.546139985981256	40.0	37.0	40.0	33.0	40.0
49	37.58999282324962	40.0	37.0	40.0	33.0	40.0
50	37.558930179674235	40.0	37.0	40.0	33.0	40.0
51	37.36459844701464	40.0	37.0	40.0	33.0	40.0
52	37.312985307923405	40.0	37.0	40.0	33.0	40.0
53	37.02398235647013	40.0	36.0	40.0	32.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	4.0
10	63.0
11	242.0
12	593.0
13	864.0
14	1260.0
15	1724.0
16	2243.0
17	3130.0
18	4492.0
19	6475.0
20	9527.0
21	13220.0
22	18149.0
23	24063.0
24	30332.0
25	37558.0
26	46291.0
27	56383.0
28	69014.0
29	84432.0
30	103453.0
31	126595.0
32	158223.0
33	199557.0
34	267995.0
35	390474.0
36	609057.0
37	1078606.0
38	2355748.0
39	7921995.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	17.595459736345454	25.117393018995188	34.50655142755373	22.780595817105624
2	24.856545745470264	27.449198209571097	24.62418230120563	23.07007374375301
3	23.155760149713355	25.295791352720542	28.159855067229177	23.388593430336925
4	21.73948983406261	25.662072841667204	27.94418549609287	24.65425182817732
5	24.437789408185314	28.840045973487722	26.571595279436643	20.15056933889032
6	26.278637627255915	25.939760812182623	26.304280414783282	21.477321145778184
7	23.710335900695387	25.6049143121258	30.11305290562955	20.57169688154926
8	25.711919542872714	25.425613011648124	26.380437952089025	22.482029493390137
9	25.34591701926417	25.240446097876895	27.17880004381224	22.23483683904669
10	22.371287595351085	25.643844659179237	29.640603081950328	22.34426466351935
11	21.987299148627155	29.04717773703905	26.482341053625653	22.483182060708142
12	22.00682672229529	26.258999935691147	29.4145090166002	22.31966432541336
13	22.042526944381066	26.075521496334837	26.076351051156077	25.805600508128023
14	22.467207624504432	29.197958502290895	25.670324342720956	22.664509530483716
15	22.414556587531543	29.21308136009404	25.800182707614077	22.57217934476034
16	22.617628671293968	25.87818288439001	25.605362124905405	25.898826319410613
17	25.999488759311934	25.599070722411575	25.690417188258436	22.71102333001805
18	22.752376270797235	25.65406359998602	25.846109211699748	25.747450917516996
19	22.62545438314744	29.04797792708786	25.75819842422758	22.56836926553712
20	26.187313184988376	25.677995889519156	25.757691881903106	22.376999043589365
21	22.788605058786807	28.864675676366147	25.986370047227364	22.360349217619685
22	25.8210170136555	25.709210642615744	25.953136466025985	22.51663587770277
23	22.808808022220912	25.56605737700345	28.99376321598289	22.631371384792747
24	22.37660996035462	28.96606489438519	25.986707742110347	22.670617403149844
25	22.536714040854033	25.710341186354423	25.895676947567143	25.8572678252244
26	23.067915432979163	27.537710974878145	26.725297839545597	22.66907575259709
27	23.082847419761492	25.391784793804973	29.171816513632155	22.35355127280138
28	25.49522954589435	25.143593737198795	26.95691982330629	22.404256893600564
29	25.692692958122016	25.19382952163905	26.65512337462314	22.458354145615793
30	25.326220598154542	25.27077256660738	26.780041116554358	22.62296571868372
31	24.294063529510566	25.664517458972274	27.075737033764497	22.96568197775266
32	23.80898685368503	26.106266413072493	27.077454872951844	23.007291860290636
33	23.477311749051005	25.996478870137523	27.336400777461716	23.189808603349757
34	23.370115647283274	25.83763747485274	27.48715951913423	23.30508735872975
35	23.411681482662598	25.960771306858643	27.327495910221316	23.30005130025744
36	23.23313632507508	26.06495017825885	27.41161130085648	23.29030219580959
37	23.478890105569295	25.90237211568193	27.352382554858533	23.26635522389024
38	23.693590639215305	26.03620206604666	27.032952560329193	23.237254734408847
39	23.583590201680195	25.962180815935437	27.22699497656838	23.227234005815987
40	23.861975585540904	25.898341800665463	27.025949062103848	23.21373355168978
41	23.55189827103156	26.138802580928576	27.075076326384746	23.23422282165511
42	23.45312251775908	26.14826537884521	27.182845041214925	23.215767062180788
43	23.529742550230644	25.921510606115334	27.311381991348547	23.237364852305472
44	23.63253393613338	25.866782011492784	27.121560761146647	23.379123291227184
45	23.44016531192289	25.843972924505227	27.254230803000258	23.46163096057163
46	23.50534776553169	25.872420047799977	27.135193356748804	23.48703882991953
47	23.750367426715073	25.8894662981975	26.975346218008184	23.384820057079246
48	23.460852794102145	25.89769577567193	27.189958657336895	23.451492772889033
49	23.417826061294264	26.091040778565834	27.015957698283422	23.47517546185648
50	23.638289431530307	26.06036193256615	26.97618311402253	23.325165521881015
51	23.607294914226966	25.965447646868643	26.99333214112357	23.433925297780817
52	23.58627707835784	25.934732094903424	26.965890761284662	23.51310006545408
53	23.678710040784733	26.055384603638704	26.779277632471093	23.486627723105467
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9892.0
1	6242.0
2	2592.0
3	1998.5
4	1405.0
5	1420.5
6	1436.0
7	1636.5
8	2150.0
9	2463.0
10	3514.5
11	4566.0
12	6691.5
13	8817.0
14	10771.0
15	12725.0
16	18070.5
17	23416.0
18	31900.5
19	40385.0
20	54193.5
21	68002.0
22	151005.0
23	234008.0
24	196870.5
25	177202.0
26	194671.0
27	217827.5
28	240984.0
29	267026.0
30	293068.0
31	316048.5
32	339029.0
33	371019.5
34	403010.0
35	444075.0
36	485140.0
37	525620.0
38	566100.0
39	603572.0
40	641044.0
41	683497.5
42	765548.0
43	805145.0
44	842683.0
45	880221.0
46	906629.0
47	933037.0
48	949169.0
49	965301.0
50	962657.0
51	960013.0
52	934695.0
53	909377.0
54	877674.5
55	845972.0
56	796366.0
57	746760.0
58	677683.0
59	540488.0
60	472370.0
61	418992.0
62	365614.0
63	315736.0
64	265858.0
65	218854.0
66	171850.0
67	140072.5
68	108295.0
69	85535.0
70	62775.0
71	46417.0
72	30059.0
73	21966.0
74	13873.0
75	10716.5
76	5772.0
77	3984.0
78	3011.0
79	2038.0
80	1736.0
81	1434.0
82	1118.0
83	802.0
84	538.0
85	274.0
86	197.0
87	120.0
88	94.5
89	69.0
90	57.0
91	45.0
92	33.5
93	15.5
94	9.0
95	7.5
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.3621764E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.34712910831932
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.05624761637958	38.76631955252287
2	14.030735664287846	14.689374626063529
3	4.652555848735367	7.306438250922493
4	2.170502574740305	4.54478314039481
5	1.2327914661022974	3.226654701984562
6	0.769137758503676	2.4157292127885155
7	0.5271455671477169	1.9316189943651825
8	0.4037781377468479	1.690930104620079
9	0.2991560803179483	1.4093965763948149
>10	1.7535012787085997	16.568310505628844
>50	0.07628917188382832	2.6340478567682077
>100	0.025348449490768483	2.627301111449753
>500	0.0023735265859048623	0.8035594037596506
>1k	3.2412925171679025E-4	0.276299190524353
>5k	7.045632353460299E-5	0.2506933967749021
>10k+	4.22737941207618E-5	0.8585433750375928
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	94868	0.69644430780037	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6884744149142503E-4	0.0	0.0	0.0	0.0
2	2.2023579324968485E-4	7.341193108322828E-6	0.0	0.0	0.0
3	2.2757698635800767E-4	7.341193108322828E-6	0.0	0.0	0.0
4	2.4225937257465332E-4	7.341193108322828E-6	0.0	0.0	0.0
5	7.2677811772396E-4	7.341193108322828E-6	0.0	7.341193108322828E-6	1.4682386216645656E-5
6	7.634840832655742E-4	1.4682386216645656E-5	0.0	7.341193108322828E-6	1.4682386216645656E-5
7	8.075312419155111E-4	1.4682386216645656E-5	0.0	7.341193108322828E-6	1.4682386216645656E-5
8	8.148724350238339E-4	1.4682386216645656E-5	0.0	7.341193108322828E-6	1.4682386216645656E-5
9	8.295548212404796E-4	1.4682386216645656E-5	0.0	7.341193108322828E-6	2.2023579324968486E-5
10	8.882843661070622E-4	1.4682386216645656E-5	0.0	7.341193108322828E-6	2.2023579324968486E-5
11	9.39672717865322E-4	1.4682386216645656E-5	0.0	7.341193108322828E-6	2.2023579324968486E-5
12	0.0010204258420568732	1.4682386216645656E-5	0.0	7.341193108322828E-6	2.2023579324968486E-5
13	0.0011158613524650698	1.4682386216645656E-5	0.0	1.4682386216645656E-5	5.13883517582598E-5
14	0.0011745908973316524	1.4682386216645656E-5	0.0	1.4682386216645656E-5	5.13883517582598E-5
15	0.0012480028284148808	1.4682386216645656E-5	0.0	2.2023579324968486E-5	5.8729544866582625E-5
16	0.0012920499870648177	1.4682386216645656E-5	0.0	2.9364772433291312E-5	5.8729544866582625E-5
17	0.001321414759498109	1.4682386216645656E-5	0.0	2.9364772433291312E-5	8.07531241915511E-5
18	0.0013728031112563688	1.4682386216645656E-5	0.0	2.9364772433291312E-5	8.809431729987394E-5
19	0.0014315326561229514	1.4682386216645656E-5	0.0	3.670596554161414E-5	8.809431729987394E-5
20	0.0014829210078812112	1.4682386216645656E-5	0.0	3.670596554161414E-5	1.027767035165196E-4
21	0.0015049445872061798	2.2023579324968486E-5	0.0	3.670596554161414E-5	1.1011789662484242E-4
22	0.0015269681665311483	2.2023579324968486E-5	0.0	3.670596554161414E-5	1.1011789662484242E-4
23	0.0015930389045060538	2.9364772433291312E-5	0.0	3.670596554161414E-5	1.2480028284148808E-4
24	0.0016517684493726364	2.9364772433291312E-5	0.0	3.670596554161414E-5	1.3948266905813373E-4
25	0.0017251803804558645	2.9364772433291312E-5	0.0	4.404715864993697E-5	1.3948266905813373E-4
26	0.00179125111843077	2.9364772433291312E-5	0.0	4.404715864993697E-5	1.3948266905813373E-4
27	0.001835298277080707	2.9364772433291312E-5	0.0	5.13883517582598E-5	1.4682386216645655E-4
28	0.0018646630495139983	2.9364772433291312E-5	0.0	5.13883517582598E-5	1.4682386216645655E-4
29	0.001923392594380581	3.670596554161414E-5	0.0	7.341193108322828E-5	1.615062483831022E-4
30	0.001996804525463809	3.670596554161414E-5	0.0	7.341193108322828E-5	1.6884744149142503E-4
31	0.0020188281047887778	3.670596554161414E-5	0.0	7.341193108322828E-5	1.761886345997479E-4
32	0.002084898842763683	3.670596554161414E-5	0.0	7.341193108322828E-5	1.8352982770807071E-4
33	0.00212894600141362	3.670596554161414E-5	0.0	7.341193108322828E-5	1.9087102081639354E-4
34	0.002187675546280203	3.670596554161414E-5	0.0	7.341193108322828E-5	1.9821221392471637E-4
35	0.002261087477363431	3.670596554161414E-5	0.0	7.341193108322828E-5	2.1289460014136202E-4
36	0.0024299349188548563	3.670596554161414E-5	0.0	7.341193108322828E-5	2.1289460014136202E-4
37	0.002481323270613116	3.670596554161414E-5	0.0	7.341193108322828E-5	2.2023579324968485E-4
38	0.002606123553454604	3.670596554161414E-5	0.0	7.341193108322828E-5	2.2023579324968485E-4
39	0.002730923836296092	3.670596554161414E-5	0.0	7.341193108322828E-5	2.2023579324968485E-4
40	0.002819018153595966	3.670596554161414E-5	0.0	8.809431729987394E-5	2.2757698635800767E-4
41	0.0029217948571124857	3.670596554161414E-5	0.0	8.809431729987394E-5	2.4225937257465332E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	57835	0.0	39.088787	1
AGCAGTG	65240	0.0	35.18156	2
GCAGTGG	70350	0.0	32.796448	3
AGTGGTA	70300	0.0	32.786343	5
TCAACGC	69975	0.0	32.673313	12
CAACGCA	70075	0.0	32.596508	13
GTGGTAT	70795	0.0	32.56374	6
GGTATCA	71015	0.0	32.45293	8
AACGCAG	70535	0.0	32.40058	14
ACGCAGA	70610	0.0	32.36284	15
GTATCAA	71195	0.0	32.351078	9
TGGTATC	71425	0.0	32.253483	7
ATCAACG	70940	0.0	32.23548	11
CAGTGGT	72065	0.0	31.980087	4
TATCAAC	71515	0.0	31.976297	10
CGCAGAG	71820	0.0	31.811054	16
AGAGTAC	71460	0.0	31.77729	19
CAGAGTA	73145	0.0	31.209103	18
GCAGAGT	74725	0.0	30.693876	17
GAGTACT	22020	0.0	30.586283	20
>>END_MODULE