FastQCFastQC Report
Thu 26 May 2016
SRR805182_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805182_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20024832
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6205943.0991221299634377No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC618000.3086168213546061No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG273820.13674022333870267No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA264760.13221584081204776No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG228460.11408834790723836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3044300.045.4623071
AGCAGTG3126500.044.280572
GTGGTAT3170300.043.7673876
AGTGGTA3172300.043.7531285
GTATCAA3169150.043.711349
GCAGTGG3177650.043.7001723
GGTATCA3172300.043.6664548
TCAACGC3148350.043.65378612
ACGCAGA3157550.043.63897715
CAACGCA3149650.043.6327913
TGGTATC3179900.043.6293457
AACGCAG3153400.043.60623614
TATCAAC3158950.043.5526810
ATCAACG3157700.043.54387711
CGCAGAG3175100.043.3948116
AGAGTAC3164050.043.3398819
CAGTGGT3201550.043.3299034
CAGAGTA3189850.043.13005418
GAGTACT995300.042.98377620
GCAGAGT3221400.042.89293717