##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805182_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20024832
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.40593963534875	37.0	37.0	37.0	35.0	37.0
2	36.2774810794917	37.0	37.0	37.0	35.0	37.0
3	38.243617025101635	39.0	39.0	39.0	37.0	39.0
4	38.18845776084414	39.0	39.0	39.0	37.0	39.0
5	38.156542986228295	39.0	39.0	39.0	37.0	39.0
6	38.13869369790468	39.0	39.0	39.0	37.0	39.0
7	38.11172817829383	39.0	39.0	39.0	37.0	39.0
8	39.1088767186661	40.0	40.0	40.0	37.0	40.0
9	39.117902312488816	40.0	40.0	40.0	38.0	40.0
10	38.92977284403685	40.0	39.0	40.0	37.0	40.0
11	39.08642199844673	40.0	40.0	40.0	37.0	40.0
12	39.11070489879765	40.0	40.0	40.0	37.0	40.0
13	39.16469546411176	40.0	40.0	40.0	38.0	40.0
14	39.13247606771433	40.0	40.0	40.0	38.0	40.0
15	39.15601449240623	40.0	40.0	40.0	38.0	40.0
16	38.97880376724259	40.0	40.0	40.0	37.0	40.0
17	39.04427457868311	40.0	40.0	40.0	37.0	40.0
18	39.054382179086446	40.0	40.0	40.0	37.0	40.0
19	39.02915799743039	40.0	40.0	40.0	37.0	40.0
20	39.02055727608601	40.0	40.0	40.0	37.0	40.0
21	38.95703239857393	40.0	40.0	40.0	37.0	40.0
22	38.99358476515558	40.0	40.0	40.0	37.0	40.0
23	38.664468745605454	40.0	39.0	40.0	36.0	40.0
24	38.75789285023715	40.0	39.0	40.0	37.0	40.0
25	38.8294110532363	40.0	40.0	40.0	37.0	40.0
26	38.82320975277096	40.0	40.0	40.0	37.0	40.0
27	38.80754480237337	40.0	40.0	40.0	37.0	40.0
28	38.73335147081384	40.0	40.0	40.0	36.0	40.0
29	38.696462122628546	40.0	40.0	40.0	36.0	40.0
30	38.65038333405244	40.0	40.0	40.0	36.0	40.0
31	38.54912026228235	40.0	39.0	40.0	35.0	40.0
32	38.43939599593145	40.0	39.0	40.0	35.0	40.0
33	38.39580921328079	40.0	39.0	40.0	35.0	40.0
34	38.39041331283079	40.0	39.0	40.0	35.0	40.0
35	38.32059474955895	40.0	39.0	40.0	35.0	40.0
36	38.226304020927614	40.0	38.0	40.0	35.0	40.0
37	38.12624515401677	40.0	38.0	40.0	35.0	40.0
38	37.48364240958426	40.0	37.0	40.0	33.0	40.0
39	37.713541916356654	40.0	37.0	40.0	33.0	40.0
40	37.528437042567944	40.0	37.0	40.0	33.0	40.0
41	37.57703490346386	40.0	37.0	40.0	33.0	40.0
42	37.552302061760116	40.0	37.0	40.0	33.0	40.0
43	37.5413052653825	40.0	37.0	40.0	33.0	40.0
44	37.608231220117105	40.0	37.0	40.0	33.0	40.0
45	37.56942650005753	40.0	37.0	40.0	33.0	40.0
46	37.56165534871903	40.0	37.0	40.0	33.0	40.0
47	37.3313940411585	40.0	36.0	40.0	33.0	40.0
48	37.4838039590045	40.0	37.0	40.0	33.0	40.0
49	37.485729867796145	40.0	37.0	40.0	33.0	40.0
50	37.42307326223761	40.0	36.0	40.0	33.0	40.0
51	37.18684256626972	40.0	36.0	40.0	33.0	40.0
52	37.03026627139743	40.0	36.0	40.0	33.0	40.0
53	36.66214238401601	39.0	35.0	40.0	32.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	24.0
13	60.0
14	199.0
15	561.0
16	1382.0
17	2439.0
18	3943.0
19	6304.0
20	9587.0
21	13907.0
22	19694.0
23	26270.0
24	34457.0
25	43987.0
26	55504.0
27	69769.0
28	87904.0
29	109381.0
30	134331.0
31	166158.0
32	208354.0
33	270015.0
34	370439.0
35	569468.0
36	934914.0
37	1788586.0
38	3523545.0
39	1.1573648E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.71702893687198	37.855613470315255	28.735656808506562	19.691700784306203
2	23.29029776629337	40.96825381606198	17.585900346130245	18.155548071514406
3	37.23887421377617	23.41366459404004	19.570701017616525	19.776760174567258
4	20.87533618259569	22.087760836145844	20.748423757063232	36.28847922419524
5	20.761277797486642	37.20147065403595	22.256790968333718	19.780460580143693
6	35.88528483035463	22.431409162383982	20.999412129899518	20.683893877361868
7	21.30493778924088	21.902600730932477	37.0628028240137	19.729658655812944
8	35.1029611634195	23.12449862251029	22.043186180038866	19.729354034031346
9	34.790828707077296	22.760865109879575	22.575794893060774	19.87251128998236
10	19.895003363823477	22.987968138758916	37.158469044833936	19.95855945258367
11	19.758602718864257	37.594992057860956	22.597008554179133	20.04939666909565
12	19.703346325202627	23.016322933445835	37.1266335717573	20.15369716959423
13	19.6662423934443	23.078365900897445	22.54854372810718	34.70684797755108
14	20.20787490252103	37.64771659507555	22.230333817532152	19.914074684871263
15	19.93640196332234	37.463959747577405	22.279727490347984	20.319910798752268
16	20.359631481552505	22.632958918207155	22.380866915637547	34.626542684602796
17	34.919823547083936	22.50792915516095	22.41312686168853	20.15912043606658
18	20.101591863542225	22.710997026092404	22.435993470507018	34.75141763985835
19	20.230641635345556	37.43392204239216	22.400452598054255	19.934983724208024
20	35.018196407340646	22.712025748830253	22.488982679105625	19.78079516472348
21	20.22606232102222	37.293061934302365	22.522306304492343	19.95856944018307
22	34.68612370880315	22.533956839188463	22.749504215565953	20.030415236442433
23	20.171719792705378	22.69968107597607	37.124541169683724	20.00405796163483
24	20.06579131350515	37.10225883542993	22.533836987995702	20.29811286306921
25	20.021785950563782	22.6585870982588	22.674976748868605	34.64465020230881
26	20.381938784804788	32.36044127611158	27.216398120094095	20.041221818989545
27	20.55193771413413	22.42476740878525	37.06511994707372	19.9581749300069
28	30.52997398430109	22.347448408056557	27.186839819679886	19.935737787962466
29	30.584301531218838	22.445916150507532	27.019867132967708	19.949915185305926
30	30.303919653358392	22.436582738871415	27.068741450614915	20.190756157155278
31	26.08279560098182	24.02498058410677	28.18628890369717	21.705934911214236
32	23.814691678811588	25.774842954987086	28.76579938348546	21.64466598271586
33	23.368655477359308	25.072480008821046	29.208449788742296	22.350414725077343
34	22.893045994093733	24.639897103755974	29.841683565684846	22.625373336465447
35	22.634307244125694	24.964094580169263	29.643304872670097	22.75829330303495
36	22.566216785239448	25.07973599978267	29.717612612180716	22.636434602797166
37	23.091579494899133	24.710539394288052	29.868924743039045	22.328956367773774
38	23.38288780649945	24.869092534708905	29.461136053476007	22.28688360531564
39	23.380705516031295	24.72017742770576	29.533551142900976	22.36556591336197
40	23.17855650424433	24.92136762995065	29.263671225806036	22.63640463999898
41	23.05932454264785	25.48513765308992	29.19792285897829	22.257614945283937
42	23.116518530592415	25.31847957575874	29.307137258379996	22.257864635268852
43	23.070845238551815	24.91256356108256	29.856824766370078	22.15976643399555
44	23.080193631587022	24.83731199342896	29.357964151709236	22.724530223274783
45	23.029821174030324	24.52541424567257	29.396830894761067	23.047933685536037
46	22.945975277095958	24.644666182467848	29.330902751144176	23.078455789292015
47	23.401779350758098	24.762300128160874	29.017821472859296	22.818099048221725
48	23.170781158114085	24.719558196543172	29.25547140670144	22.854189238641304
49	22.606656575196237	25.380887090588327	29.154401894607656	22.858054439607784
50	23.194941161054437	25.03050712235688	29.095529989964458	22.679021726624224
51	23.106026557426297	25.003855213367082	28.936082959397613	22.954035269809005
52	23.269089098974714	24.96394975997801	28.783077930441564	22.98388321060571
53	23.55268698384086	25.16473546444734	28.19208171134719	23.090495840364603
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5983.0
1	3712.0
2	1441.0
3	1169.5
4	898.0
5	929.5
6	961.0
7	1122.0
8	1591.0
9	1899.0
10	2861.5
11	3824.0
12	5753.0
13	7682.0
14	9677.5
15	11673.0
16	17272.5
17	22872.0
18	31993.0
19	41114.0
20	59899.5
21	78685.0
22	451060.0
23	823435.0
24	549685.0
25	272300.5
26	268666.0
27	287212.5
28	305759.0
29	332651.5
30	359544.0
31	387754.5
32	415965.0
33	460148.5
34	504332.0
35	560558.5
36	616785.0
37	678369.5
38	739954.0
39	800487.5
40	861021.0
41	928749.5
42	1063198.5
43	1129919.0
44	1202239.0
45	1274559.0
46	1326649.5
47	1378740.0
48	1442969.5
49	1507199.0
50	1486887.5
51	1466576.0
52	1429401.5
53	1392227.0
54	1345940.0
55	1299653.0
56	1233659.5
57	1167666.0
58	1043510.0
59	806359.5
60	693365.0
61	606969.0
62	520573.0
63	447556.5
64	374540.0
65	306032.0
66	237524.0
67	192739.0
68	147954.0
69	116597.0
70	85240.0
71	63255.0
72	41270.0
73	30639.0
74	20008.0
75	15317.0
76	7951.0
77	5276.0
78	3957.5
79	2639.0
80	2307.5
81	1976.0
82	1510.5
83	1045.0
84	717.0
85	389.0
86	270.5
87	152.0
88	124.0
89	96.0
90	67.0
91	38.0
92	27.5
93	15.0
94	13.0
95	9.5
96	6.0
97	4.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.0024832E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.280883464171566
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.4664752040319	30.931321849008114
2	14.650302649388102	12.68156083366021
3	5.097496263197606	6.61872425179517
4	2.3631833071297765	4.091226452814377
5	1.3196355895833631	2.855749708396544
6	0.8385925256192244	2.1777015225150578
7	0.6054462082684791	1.8342972748724813
8	0.4478275805797647	1.5505898661691757
9	0.3591574265837964	1.3990185652738543
>10	2.6749141023823633	22.095126553726146
>50	0.13036491816777074	3.765652907046206
>100	0.04053650323858949	3.2720489835970143
>500	0.004644356326851284	1.3612800938518297
>1k	0.0011921175797945625	0.8866059882234626
>5k	1.1562396137892162E-4	0.3342626992975378
>10k+	1.1562396137889647E-4	4.1448324497527995
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	620594	3.0991221299634377	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	61800	0.3086168213546061	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	27382	0.13674022333870267	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA	26476	0.13221584081204776	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	22846	0.11408834790723836	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.142373628902354E-4	0.0	0.0	0.0	0.0
2	0.0010586855360384547	0.0	0.0	0.0	0.0
3	0.0010836545345299276	0.0	0.0	0.0	4.993799698294597E-6
4	0.0010886483342282221	0.0	0.0	0.0	1.4981399094883793E-5
5	0.001093642133926517	0.0	0.0	4.993799698294597E-6	1.997519879317839E-5
6	0.001153567730306052	0.0	0.0	4.993799698294597E-6	2.4968998491472987E-5
7	0.0011735429290992304	0.0	0.0	9.987599396589194E-6	3.495659788806218E-5
8	0.0011885243281941143	0.0	0.0	9.987599396589194E-6	4.9937996982945975E-5
9	0.0012384623251770603	0.0	0.0	9.987599396589194E-6	4.9937996982945975E-5
10	0.0012384623251770603	0.0	0.0	1.4981399094883793E-5	5.493179668124057E-5
11	0.0012584375239702386	0.0	0.0	1.4981399094883793E-5	5.992559637953517E-5
12	0.0012784127227634169	0.0	0.0	1.997519879317839E-5	6.991319577612436E-5
13	0.001313369320651479	0.0	0.0	2.4968998491472987E-5	8.489459487100816E-5
14	0.0013483259185395413	0.0	0.0	2.4968998491472987E-5	8.988839456930276E-5
15	0.0013782887167293088	0.0	0.0	2.4968998491472987E-5	8.988839456930276E-5
16	0.0013932701158241927	0.0	0.0	2.9962798189767586E-5	9.987599396589195E-5
17	0.001463183311600317	0.0	0.0	3.995039758635678E-5	1.1985119275907034E-4
18	0.0014931461097900847	0.0	0.0	3.995039758635678E-5	1.2484499245736495E-4
19	0.0015031337091866738	4.993799698294597E-6	0.0	4.494419728465138E-5	1.3483259185395412E-4
20	0.0015830345043593875	4.993799698294597E-6	0.0	4.9937996982945975E-5	1.5480779064713253E-4
21	0.0016179911022474495	4.993799698294597E-6	0.0	4.9937996982945975E-5	1.598015903454271E-4
22	0.0016479539004372172	4.993799698294597E-6	0.0	5.992559637953517E-5	1.647953900437217E-4
23	0.0017078794968167523	4.993799698294597E-6	0.0	5.992559637953517E-5	1.847705888369001E-4
24	0.0017927740916877605	4.993799698294597E-6	0.0	5.992559637953517E-5	1.897643885351947E-4
25	0.0018427120886707065	4.993799698294597E-6	0.0	5.992559637953517E-5	1.897643885351947E-4
26	0.0018726748868604741	4.993799698294597E-6	0.0	5.992559637953517E-5	1.997519879317839E-4
27	0.00192261288384342	4.993799698294597E-6	0.0	5.992559637953517E-5	2.047457876300785E-4
28	0.0019525756820331875	4.993799698294597E-6	0.0	6.991319577612436E-5	2.1972718672496228E-4
29	0.0020075074787144283	4.993799698294597E-6	0.0	7.990079517271355E-5	2.2472098642325688E-4
30	0.002032476477205901	4.993799698294597E-6	4.993799698294597E-6	7.990079517271355E-5	2.347085858198461E-4
31	0.002087408273887142	4.993799698294597E-6	4.993799698294597E-6	8.489459487100816E-5	2.397023855181407E-4
32	0.002167309069059855	9.987599396589194E-6	4.993799698294597E-6	8.988839456930276E-5	2.496899849147299E-4
33	0.0022921540615172204	9.987599396589194E-6	9.987599396589194E-6	8.988839456930276E-5	2.6966518370790825E-4
34	0.0023520796578967553	9.987599396589194E-6	9.987599396589194E-6	8.988839456930276E-5	2.7965278310449745E-4
35	0.0024269866533711743	9.987599396589194E-6	9.987599396589194E-6	9.987599396589195E-5	2.8964038250108666E-4
36	0.002466937050957531	9.987599396589194E-6	9.987599396589194E-6	1.0486979366418655E-4	3.0462178159597044E-4
37	0.0025018936488455934	9.987599396589194E-6	9.987599396589194E-6	1.0486979366418655E-4	3.196031806908542E-4
38	0.002551831645828539	9.987599396589194E-6	9.987599396589194E-6	1.0486979366418655E-4	3.295907800874434E-4
39	0.00260676344250978	9.987599396589194E-6	9.987599396589194E-6	1.0486979366418655E-4	3.295907800874434E-4
40	0.002706639436475672	9.987599396589194E-6	9.987599396589194E-6	1.0486979366418655E-4	3.3458457978573805E-4
41	0.0028065154304415638	9.987599396589194E-6	9.987599396589194E-6	1.0486979366418655E-4	3.445721791823272E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	304430	0.0	45.462307	1
AGCAGTG	312650	0.0	44.28057	2
GTGGTAT	317030	0.0	43.767387	6
AGTGGTA	317230	0.0	43.753128	5
GTATCAA	316915	0.0	43.71134	9
GCAGTGG	317765	0.0	43.700172	3
GGTATCA	317230	0.0	43.666454	8
TCAACGC	314835	0.0	43.653786	12
ACGCAGA	315755	0.0	43.638977	15
CAACGCA	314965	0.0	43.63279	13
TGGTATC	317990	0.0	43.629345	7
AACGCAG	315340	0.0	43.606236	14
TATCAAC	315895	0.0	43.55268	10
ATCAACG	315770	0.0	43.543877	11
CGCAGAG	317510	0.0	43.39481	16
AGAGTAC	316405	0.0	43.33988	19
CAGTGGT	320155	0.0	43.329903	4
CAGAGTA	318985	0.0	43.130054	18
GAGTACT	99530	0.0	42.983776	20
GCAGAGT	322140	0.0	42.892937	17
>>END_MODULE