FastQCFastQC Report
Thu 26 May 2016
SRR805181_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805181_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14918215
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2064831.384099907395087No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC195100.13077972130043708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1027550.044.337941
AGCAGTG1101050.041.5958862
AGTGGTA1143850.039.8751145
CAACGCA1142600.039.84675213
TCAACGC1138000.039.8467512
GCAGTGG1150650.039.7620053
AACGCAG1146850.039.7236814
GTGGTAT1144350.039.72016
ACGCAGA1147950.039.67947415
GGTATCA1149700.039.6272478
GTATCAA1151150.039.6099979
ATCAACG1151100.039.51168411
TGGTATC1152000.039.4400187
TATCAAC1163150.039.28014410
CGCAGAG1163050.039.15218716
AGAGTAC1151400.039.09727519
CAGTGGT1169800.039.0588574
CAGAGTA1168000.038.61605518
GAGTACT387900.038.46996720
GCAGAGT1182350.038.19905517
Produced by FastQC (version 0.11.5)