##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805181_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14918215
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.03193927691751	37.0	35.0	37.0	35.0	37.0
2	36.10821462219173	37.0	35.0	37.0	35.0	37.0
3	37.962338858905035	39.0	38.0	39.0	35.0	39.0
4	37.93146076792699	39.0	38.0	39.0	35.0	39.0
5	37.90774298399641	39.0	38.0	39.0	35.0	39.0
6	37.88379755889026	39.0	38.0	39.0	35.0	39.0
7	37.81272853354104	39.0	38.0	39.0	35.0	39.0
8	38.750630219500124	40.0	39.0	40.0	36.0	40.0
9	38.636344629702684	40.0	39.0	40.0	36.0	40.0
10	38.56097569313755	40.0	38.0	40.0	36.0	40.0
11	38.61341936686125	40.0	39.0	40.0	36.0	40.0
12	38.45007730482501	40.0	38.0	40.0	36.0	40.0
13	38.66359145514393	40.0	39.0	40.0	36.0	40.0
14	38.68602450092052	40.0	39.0	40.0	36.0	40.0
15	38.642342398202466	40.0	39.0	40.0	36.0	40.0
16	38.601784261723004	40.0	39.0	40.0	36.0	40.0
17	38.15308822134551	40.0	38.0	40.0	34.0	40.0
18	38.28986054967032	40.0	38.0	40.0	35.0	40.0
19	38.381519169686186	40.0	38.0	40.0	35.0	40.0
20	38.319120953813844	40.0	38.0	40.0	35.0	40.0
21	38.30530355005609	40.0	38.0	40.0	35.0	40.0
22	38.22738363805589	40.0	38.0	40.0	35.0	40.0
23	37.796884412779946	40.0	38.0	40.0	34.0	40.0
24	37.79968541812811	40.0	38.0	40.0	34.0	40.0
25	37.770022284837694	39.0	38.0	40.0	34.0	40.0
26	37.53941614328524	39.0	37.0	40.0	34.0	40.0
27	37.52933879824094	39.0	37.0	40.0	33.0	40.0
28	37.46207069679583	39.0	37.0	40.0	33.0	40.0
29	37.24389211443862	39.0	37.0	40.0	33.0	40.0
30	37.14966140386098	38.0	37.0	40.0	33.0	40.0
31	37.009245073891215	38.0	36.0	40.0	33.0	40.0
32	36.81308682037362	38.0	36.0	40.0	32.0	40.0
33	36.71467806302564	38.0	36.0	40.0	32.0	40.0
34	36.601616949480885	38.0	36.0	40.0	32.0	40.0
35	36.34427114772109	38.0	35.0	40.0	31.0	40.0
36	36.19051649275735	38.0	35.0	39.0	31.0	40.0
37	36.00418944223555	38.0	35.0	39.0	31.0	40.0
38	35.91289990122813	38.0	35.0	39.0	31.0	40.0
39	35.777028149815514	38.0	35.0	39.0	30.0	40.0
40	35.68990184147366	37.0	35.0	39.0	30.0	40.0
41	35.45838841979419	37.0	34.0	38.0	30.0	40.0
42	35.28540277774519	37.0	34.0	38.0	30.0	40.0
43	34.976740850027966	37.0	34.0	38.0	29.0	40.0
44	34.83656107650949	36.0	34.0	38.0	29.0	39.0
45	34.75208984452899	36.0	33.0	38.0	29.0	39.0
46	34.64816776001687	36.0	33.0	38.0	29.0	39.0
47	34.440398868095144	36.0	33.0	38.0	28.0	39.0
48	34.272938753061275	36.0	33.0	38.0	28.0	39.0
49	33.934523064589165	36.0	33.0	38.0	27.0	39.0
50	33.69980396448235	35.0	32.0	38.0	27.0	39.0
51	33.38549524859375	35.0	32.0	38.0	26.0	39.0
52	32.888081985679925	34.0	31.0	37.0	26.0	38.0
53	35.04386396093634	37.0	34.0	39.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	12.0
13	52.0
14	211.0
15	639.0
16	1357.0
17	2234.0
18	3333.0
19	5257.0
20	8564.0
21	13636.0
22	19929.0
23	28280.0
24	39098.0
25	52514.0
26	69303.0
27	89335.0
28	114475.0
29	144779.0
30	184684.0
31	236651.0
32	308223.0
33	418815.0
34	603456.0
35	928606.0
36	1636226.0
37	3514481.0
38	6008968.0
39	485094.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.762482978023844	30.027372577751425	22.16992448493335	26.040219959291377
2	23.39057990516962	30.43888963927655	22.20897071130829	23.96155974424554
3	25.901718134508723	23.953770608615038	26.55650156536824	23.588009691508
4	21.343558864113437	24.11450029376839	26.6088335635329	27.933107278585272
5	23.83627665910432	30.397209049474082	25.680518748389137	20.08599554303246
6	28.656551738931235	24.427279000872424	25.812337467987962	21.103831792208382
7	23.312326575263864	24.799709616733637	31.60974017333843	20.278223634664066
8	28.489447296476158	24.885242637942945	25.09796916051954	21.52734090506136
9	27.738546468193416	24.55817938003977	26.119659758221747	21.583614393545073
10	21.610775820029406	24.753919956241415	31.69637252177958	21.938931701949596
11	21.554904524435397	31.272689125341067	25.111248229094436	22.061158121129104
12	21.77874497719734	24.989383783515656	31.383734582186946	21.848136657100063
13	21.68741367516154	25.20476477916426	24.68301334978749	28.42480819588671
14	21.922756844568873	31.63572183401298	24.731390451203445	21.7101308702147
15	21.898745929053845	31.18771247096251	24.91395250705262	21.999589092931025
16	21.853646699688937	24.827943557590505	24.823894815834198	28.49451492688636
17	28.429386491614444	24.986789639377097	24.717085790759818	21.866738078248638
18	21.838564466325227	24.8721177433091	25.08184122564261	28.20747656472306
19	22.150438239427437	31.27844048366376	24.84738958380745	21.723731693101353
20	28.615930257071643	24.66017549686742	24.939471645904018	21.784422600156923
21	21.865792924957844	31.13389906232079	25.16111344420227	21.839194568519087
22	28.377228777035324	24.619332808918493	24.940269328468588	22.0631690855776
23	22.20470746667748	24.84543224507758	31.02870551202004	21.921154776224906
24	21.778416519670753	31.213767867000175	24.894834938362266	22.112980674966813
25	22.111016633022114	24.639140808736165	24.896504038854513	28.353338519387204
26	22.651543767133	28.768549052282726	26.879951790478955	21.69995539010532
27	22.49032474729718	24.46278592981801	31.226577710537086	21.820311612347723
28	26.890093754514194	24.414053558016157	26.815393128467445	21.8804595590022
29	26.946052191900975	24.534597470273756	26.758697337449554	21.760653000375715
30	26.405947360324273	24.534034400228176	27.017689448771183	22.042328790676365
31	24.802290354442537	25.17986233607707	27.326714355571358	22.69113295390903
32	23.831604518368987	26.010102415067752	27.544287302468828	22.61400576409443
33	23.39515820089736	25.63765168956206	27.94540767779523	23.02178243174535
34	23.278763578618488	25.464105457656967	28.08992228627889	23.16720867744566
35	23.27922610044164	25.664578503527398	28.014966938068664	23.041228457962294
36	23.047127287011214	25.687188447143306	28.1678337522284	23.097850513617075
37	23.486435877214532	25.45787146786663	27.981437457497428	23.074255197421405
38	23.46786126892527	25.628495098106573	27.987074861168043	22.91656877180011
39	23.516426060356416	25.547513559765694	27.95465141104348	22.98140896883441
40	23.404790720605646	25.622173966523476	27.74023567833015	23.232799634540726
41	23.395526877712918	25.95721405007235	27.66912797543138	22.97813109678336
42	23.329728120958173	25.857316039485955	27.86849499085514	22.944460848700732
43	23.48437799026224	25.564559835074103	27.913011040529984	23.038051134133674
44	23.44084731316716	25.598618869616775	27.78423558046321	23.176298236752853
45	23.342651919147166	25.446462596228837	27.900670422031055	23.310215062592945
46	23.440545668499883	25.476700798319367	27.687166326534374	23.395587206646372
47	23.395828522380192	25.57875054086565	27.70822782752494	23.31719310922922
48	23.29717730975187	25.50886952628046	27.863641863319437	23.330311300648233
49	23.303129764519415	25.810185736028068	27.60017870770732	23.286505791745192
50	23.578437500733166	25.761071280981003	27.57360716412788	23.086884054157956
51	23.34119732152942	25.631551764068288	27.6977305931038	23.329520321298496
52	23.704015527326828	25.623722409148815	27.352347449074838	23.319914614449516
53	23.780646679244132	25.805647659589297	27.145586787695446	23.26811887347112
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4111.0
1	2839.0
2	1567.0
3	1261.5
4	956.0
5	973.5
6	991.0
7	1134.5
8	1575.5
9	1873.0
10	2705.0
11	3537.0
12	5090.5
13	6644.0
14	8670.0
15	10696.0
16	15612.5
17	20529.0
18	28569.5
19	36610.0
20	50285.5
21	63961.0
22	202293.0
23	340625.0
24	261378.5
25	190620.5
26	199109.0
27	220277.5
28	241446.0
29	265203.0
30	288960.0
31	313172.5
32	337385.0
33	372894.0
34	408403.0
35	453410.0
36	498417.0
37	545391.0
38	592365.0
39	637345.5
40	682326.0
41	733020.0
42	829448.0
43	875182.0
44	919837.5
45	964493.0
46	997253.5
47	1030014.0
48	1055994.0
49	1081974.0
50	1077211.0
51	1072448.0
52	1047512.5
53	1022577.0
54	990070.5
55	957564.0
56	905085.5
57	852607.0
58	770999.5
59	609305.5
60	529219.0
61	466277.0
62	403335.0
63	347749.5
64	292164.0
65	241504.0
66	190844.0
67	153833.0
68	116822.0
69	91726.0
70	66630.0
71	49476.5
72	32323.0
73	23900.5
74	15478.0
75	12007.0
76	6460.0
77	4384.0
78	3255.0
79	2126.0
80	1765.5
81	1405.0
82	1059.5
83	714.0
84	450.0
85	186.0
86	133.5
87	81.0
88	55.5
89	30.0
90	32.0
91	34.0
92	22.5
93	8.5
94	6.0
95	3.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.4918215E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.26763701321292
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.38808870293425	34.94001989593489
2	14.152002024667862	13.661673934738452
3	4.854700536255875	7.029747698755459
4	2.383009838431178	4.600890155212451
5	1.390990833328941	3.356992031591392
6	0.9468996990425711	2.7422766576784423
7	0.636576372060702	2.1508226080469752
8	0.5023516057746439	1.9397859968428115
9	0.38762577340271553	1.6838802114811373
>10	2.2376081037825335	19.484917252097837
>50	0.089618018366871	2.8433938291386736
>100	0.028027433141756933	2.655217425101688
>500	0.001999989694380662	0.6368699729028201
>1k	3.7581884192318475E-4	0.3358445528120549
>5k	6.958348544131711E-5	0.26087825904079204
>10k+	5.5666788353053694E-5	1.6767895186239583
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	206483	1.384099907395087	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	19510	0.13077972130043708	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.233989790333495E-4	0.0	0.0	0.0	3.351607414157793E-5
2	6.502118383466119E-4	6.703214828315586E-6	0.0	0.0	3.351607414157793E-5
3	6.770246976598742E-4	6.703214828315586E-6	0.0	0.0	4.0219288969893514E-5
4	6.971343421448209E-4	6.703214828315586E-6	0.0	6.703214828315586E-6	4.69225037982091E-5
5	0.0011864690246118587	6.703214828315586E-6	0.0	6.703214828315586E-6	5.362571862652469E-5
6	0.0012065786690968056	6.703214828315586E-6	0.0	1.3406429656631173E-5	5.362571862652469E-5
7	0.0012065786690968056	6.703214828315586E-6	0.0	1.3406429656631173E-5	6.703214828315586E-5
8	0.0012132818839251211	6.703214828315586E-6	0.0	1.3406429656631173E-5	6.703214828315586E-5
9	0.0012199850987534367	6.703214828315586E-6	0.0	1.3406429656631173E-5	7.373536311147144E-5
10	0.0012199850987534367	1.3406429656631173E-5	0.0	1.3406429656631173E-5	7.373536311147144E-5
11	0.0012736108173799613	2.6812859313262345E-5	0.0	1.3406429656631173E-5	9.38450075964182E-5
12	0.0013004236766932238	2.6812859313262345E-5	0.0	1.3406429656631173E-5	9.38450075964182E-5
13	0.0013540493953197485	3.351607414157793E-5	0.0	1.3406429656631173E-5	1.0725143725304938E-4
14	0.0013808622546330107	4.0219288969893514E-5	0.0	2.0109644484946757E-5	1.1395465208136497E-4
15	0.0013875654694613263	4.0219288969893514E-5	0.0	2.0109644484946757E-5	1.2736108173799613E-4
16	0.0013942686842896418	4.69225037982091E-5	0.0	2.0109644484946757E-5	1.3406429656631172E-4
17	0.0014009718991179574	4.69225037982091E-5	0.0	2.0109644484946757E-5	1.474707262229429E-4
18	0.0014210815436029043	4.69225037982091E-5	0.0	2.0109644484946757E-5	1.474707262229429E-4
19	0.0014344879732595354	4.69225037982091E-5	0.0	2.6812859313262345E-5	1.474707262229429E-4
20	0.001441191188087851	4.69225037982091E-5	0.0	2.6812859313262345E-5	1.5417394105125847E-4
21	0.0014613008325727978	4.69225037982091E-5	0.0	2.6812859313262345E-5	1.6087715587957406E-4
22	0.0014814104770577445	5.362571862652469E-5	0.0	2.6812859313262345E-5	1.6758037070788964E-4
23	0.0015015201215426912	6.0328933454840275E-5	0.0	3.351607414157793E-5	1.876900151928364E-4
24	0.001521629766027638	6.0328933454840275E-5	0.0	3.351607414157793E-5	1.876900151928364E-4
25	0.0015484426253409003	6.0328933454840275E-5	0.0	3.351607414157793E-5	1.9439323002115198E-4
26	0.0015618490549975316	6.0328933454840275E-5	0.0	4.0219288969893514E-5	1.9439323002115198E-4
27	0.0015752554846541627	6.0328933454840275E-5	0.0	4.0219288969893514E-5	2.010964448494676E-4
28	0.0016087715587957407	6.0328933454840275E-5	0.0	4.0219288969893514E-5	2.010964448494676E-4
29	0.001682506921907212	6.0328933454840275E-5	0.0	4.0219288969893514E-5	2.0779965967778318E-4
30	0.0016959133515638432	6.0328933454840275E-5	0.0	4.0219288969893514E-5	2.2120608933441435E-4
31	0.0017428358553620523	6.703214828315586E-5	0.0	4.0219288969893514E-5	2.2120608933441435E-4
32	0.0017562422850186834	6.703214828315586E-5	0.0	4.0219288969893514E-5	2.3461251899104552E-4
33	0.0017696487146753148	7.373536311147144E-5	6.703214828315586E-6	4.0219288969893514E-5	2.7483180796093905E-4
34	0.0018031647888168926	7.373536311147144E-5	6.703214828315586E-6	4.0219288969893514E-5	2.815350227892546E-4
35	0.0018366808629584706	7.373536311147144E-5	6.703214828315586E-6	4.0219288969893514E-5	3.0834788210251695E-4
36	0.0018366808629584706	7.373536311147144E-5	6.703214828315586E-6	4.0219288969893514E-5	3.1505109693083256E-4
37	0.0018701969371000486	7.373536311147144E-5	6.703214828315586E-6	4.0219288969893514E-5	3.1505109693083256E-4
38	0.0019238226557265732	7.373536311147144E-5	6.703214828315586E-6	4.69225037982091E-5	3.1505109693083256E-4
39	0.00194393230021152	8.043857793978703E-5	6.703214828315586E-6	4.69225037982091E-5	3.217543117591481E-4
40	0.0019908548040097293	8.043857793978703E-5	6.703214828315586E-6	4.69225037982091E-5	3.217543117591481E-4
41	0.002037777307807938	8.043857793978703E-5	6.703214828315586E-6	4.69225037982091E-5	3.217543117591481E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	102755	0.0	44.33794	1
AGCAGTG	110105	0.0	41.595886	2
AGTGGTA	114385	0.0	39.875114	5
CAACGCA	114260	0.0	39.846752	13
TCAACGC	113800	0.0	39.84675	12
GCAGTGG	115065	0.0	39.762005	3
AACGCAG	114685	0.0	39.72368	14
GTGGTAT	114435	0.0	39.7201	6
ACGCAGA	114795	0.0	39.679474	15
GGTATCA	114970	0.0	39.627247	8
GTATCAA	115115	0.0	39.609997	9
ATCAACG	115110	0.0	39.511684	11
TGGTATC	115200	0.0	39.440018	7
TATCAAC	116315	0.0	39.280144	10
CGCAGAG	116305	0.0	39.152187	16
AGAGTAC	115140	0.0	39.097275	19
CAGTGGT	116980	0.0	39.058857	4
CAGAGTA	116800	0.0	38.616055	18
GAGTACT	38790	0.0	38.469967	20
GCAGAGT	118235	0.0	38.199055	17
>>END_MODULE