FastQCFastQC Report
Thu 26 May 2016
SRR805180_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805180_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22004805
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7259803.2991885181441054No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC847830.3852931212069364No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG514330.23373531371898093No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG388150.17639329228320816No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA305120.1386606243499999No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG253060.11500215521110049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3627850.045.834021
AGCAGTG3754800.044.2774852
AGTGGTA3769050.044.085145
GTGGTAT3770950.044.079756
GTATCAA3783300.043.9420749
GGTATCA3783500.043.9360248
TGGTATC3783300.043.929037
TCAACGC3786100.043.910212
CAACGCA3790250.043.870813
ACGCAGA3792350.043.8359715
AACGCAG3796700.043.81050514
TATCAAC3799200.043.76188710
ATCAACG3802250.043.75088511
GCAGTGG3804900.043.690153
CAGTGGT3804650.043.648554
CGCAGAG3810800.043.62312316
AGAGTAC3766700.043.61852619
CAGAGTA3793450.043.45528418
GAGTACT1112050.043.225820
GCAGAGT3839750.043.135117