FastQCFastQC Report
Thu 26 May 2016
SRR805179_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805179_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20876009
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8230493.9425591357045304No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC992290.47532552797807287No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG535510.2565193375802818No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG456010.2184373459505598No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA428290.20515894585023411No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG356270.1706600145650445No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC282220.13518867519169972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT5040750.046.193941
AGCAGTG5124450.045.409162
GTGGTAT5154950.045.1418656
AGTGGTA5151650.045.1082885
GTATCAA5168350.045.038929
TGGTATC5154200.045.007097
GGTATCA5172500.044.99378
TCAACGC5203750.044.951112
TATCAAC5185000.044.9323610
CAACGCA5214950.044.92125313
GCAGTGG5177650.044.9108163
AACGCAG5235700.044.8886514
ATCAACG5215950.044.87119311
ACGCAGA5208800.044.86826715
CAGTGGT5194150.044.725174
AGAGTAC5149550.044.72372419
CGCAGAG5228350.044.6874616
CAGAGTA5193000.044.61289618
GAGTACT1505650.044.51990520
GCAGAGT5235950.044.44486217
Produced by FastQC (version 0.11.5)