##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805179_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20876009
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.7256466022792	36.0	35.0	37.0	35.0	37.0
2	36.199504081455416	37.0	36.0	37.0	35.0	37.0
3	37.85701471962385	39.0	39.0	39.0	35.0	39.0
4	37.983737217204684	39.0	39.0	39.0	35.0	39.0
5	37.90414786657737	39.0	39.0	39.0	35.0	39.0
6	37.99711736089019	39.0	39.0	39.0	35.0	39.0
7	37.85975408422175	39.0	38.0	39.0	35.0	39.0
8	38.85982943387311	40.0	39.0	40.0	36.0	40.0
9	38.87556424218825	40.0	39.0	40.0	36.0	40.0
10	38.536512462703	40.0	39.0	40.0	36.0	40.0
11	38.752139980395675	40.0	39.0	40.0	36.0	40.0
12	38.76555121239888	40.0	39.0	40.0	36.0	40.0
13	38.76554383550994	40.0	39.0	40.0	36.0	40.0
14	38.8904146860638	40.0	39.0	40.0	37.0	40.0
15	38.80850444162962	40.0	39.0	40.0	36.0	40.0
16	38.822583856904835	40.0	39.0	40.0	36.0	40.0
17	38.80045419601036	40.0	39.0	40.0	36.0	40.0
18	38.69251991604334	40.0	39.0	40.0	36.0	40.0
19	38.66905762495121	40.0	39.0	40.0	36.0	40.0
20	38.569442847049935	40.0	39.0	40.0	36.0	40.0
21	38.26408495991738	40.0	38.0	40.0	35.0	40.0
22	38.40510741301175	40.0	38.0	40.0	36.0	40.0
23	38.06779356149923	40.0	38.0	40.0	35.0	40.0
24	38.10619798065809	40.0	38.0	40.0	35.0	40.0
25	37.999004646913114	40.0	38.0	40.0	35.0	40.0
26	38.082802943800225	40.0	38.0	40.0	35.0	40.0
27	37.805355947106555	40.0	38.0	40.0	34.0	40.0
28	37.828722673955546	40.0	38.0	40.0	34.0	40.0
29	37.63384505151344	40.0	38.0	40.0	34.0	40.0
30	37.55063494176497	39.0	37.0	40.0	34.0	40.0
31	37.288540352708225	39.0	37.0	40.0	33.0	40.0
32	36.91606350619987	39.0	36.0	40.0	32.0	40.0
33	36.79757452681688	38.0	36.0	40.0	32.0	40.0
34	36.728505242549	38.0	36.0	40.0	32.0	40.0
35	36.50116686575485	38.0	36.0	40.0	31.0	40.0
36	36.36717061196898	38.0	35.0	40.0	31.0	40.0
37	35.94701750703403	38.0	35.0	39.0	31.0	40.0
38	35.83541993107974	38.0	35.0	39.0	31.0	40.0
39	35.673494775749525	38.0	35.0	39.0	30.0	40.0
40	35.51215876559547	37.0	34.0	39.0	30.0	40.0
41	35.34869188837771	37.0	34.0	38.0	30.0	40.0
42	35.09291028759376	37.0	34.0	38.0	30.0	40.0
43	34.89063158575952	36.0	34.0	38.0	29.0	40.0
44	34.87572303690806	36.0	34.0	38.0	29.0	40.0
45	34.59986211923936	36.0	33.0	38.0	28.0	40.0
46	33.60837136063699	36.0	33.0	38.0	25.0	39.0
47	33.50067965577137	35.0	32.0	38.0	25.0	39.0
48	33.69165217355482	35.0	32.0	38.0	26.0	39.0
49	33.66025311638829	35.0	32.0	38.0	27.0	39.0
50	33.474331755653104	35.0	32.0	38.0	27.0	39.0
51	32.834025507461696	35.0	31.0	37.0	25.0	39.0
52	32.35162405802757	34.0	31.0	37.0	23.0	38.0
53	34.2210095330003	36.0	33.0	39.0	26.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	15.0
12	71.0
13	157.0
14	541.0
15	1092.0
16	2275.0
17	3657.0
18	5307.0
19	8328.0
20	13237.0
21	20818.0
22	30417.0
23	42323.0
24	57729.0
25	76887.0
26	100554.0
27	128191.0
28	161544.0
29	200584.0
30	248980.0
31	311827.0
32	403377.0
33	543195.0
34	792595.0
35	1266246.0
36	2348492.0
37	4986182.0
38	8400002.0
39	721385.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.307519650906455	44.45839719651395	25.35929161555736	16.874791537022237
2	21.427165508503084	49.0527523723524	11.753300163838787	17.76678195530573
3	45.50182939660545	21.24269059282356	15.166251365383104	18.08922864518788
4	20.129312073011658	18.738428403628298	16.746553424076414	44.38570609928363
5	18.7023295496759	43.224588569587226	19.26543526590739	18.807646614829494
6	43.34598150441495	19.55400574889578	17.837068378347603	19.26294436834167
7	19.70391946085097	18.902880335029554	42.87738139986431	18.51581880425516
8	42.830509413940185	20.60753566450369	18.715833088594664	17.84612183296146
9	42.49677704201028	20.290075559940597	19.05195576415013	18.16119163389899
10	18.377942833805065	20.218567639053997	43.27319939361973	18.13029013352121
11	18.11571359257414	44.07604921036391	19.429475241172774	18.378761955889175
12	18.093846386059713	20.209389639561852	43.338111226144804	18.35865274823363
13	18.114937582178662	20.27066092949088	19.536119188298876	42.07828230003158
14	18.3377292086816	44.16978839202455	19.327271797976326	18.165210601317522
15	18.261052675346136	43.89541602516075	19.366244764504557	18.47728653498856
16	18.283097118802736	19.79785025001666	19.610410208196406	42.3086424229842
17	42.32789418705462	19.796652703110063	19.547562946538296	18.327890163297017
18	18.30178843092087	19.97193045854694	19.522318657747274	42.203962452784914
19	18.388447715269717	43.933196234970005	19.59665278933344	18.08170326042684
20	42.52112077552754	19.873755563144275	19.61679073811474	17.988332923213434
21	18.38573167888556	43.71415532537852	19.672395236081762	18.227717759654155
22	42.132310826269524	19.751150710847078	19.901969768263655	18.214568694619743
23	18.3251453857871	19.947213090394815	43.40955208440464	18.31808943941344
24	18.306789386802812	43.57150353786493	19.595091188167242	18.526615887165025
25	18.39719938806311	19.880490566946968	19.797505356507557	41.92480468848237
26	18.659184329725093	36.51438356823854	26.557178625473867	18.269253476562497
27	19.125973743352954	19.773425083309746	42.98550551496697	18.115095658370333
28	35.5776959092133	19.642710443361086	26.584746155263684	18.194847492161937
29	35.57977964083077	19.76026643790008	26.426636432279754	18.233317488989396
30	35.22828525318225	19.794506699053443	26.524179022915728	18.453029024848572
31	28.350600921852447	22.3472503772153	28.271893348963395	21.030255351968854
32	24.78453137283089	25.202633319424223	29.063955663172976	20.94887964457191
33	23.951982392803146	24.048758553418903	29.976860040633248	22.022399013144707
34	23.0483613989628	23.221138676458704	31.008915545112092	22.7215843794664
35	22.66648285120015	23.809210850598888	30.76042935218125	22.763876946019714
36	22.508210261836926	24.08860812428276	30.966239763548675	22.43694185033164
37	23.58175837153548	23.24096526304429	30.9521901432405	22.225086222179726
38	23.739154356563077	23.45645664360463	30.825317233768196	21.9790717660641
39	23.927978762607356	23.439782000477198	30.647744978458284	21.98449425845716
40	22.859479510667004	23.906044493466162	30.430835702360543	22.80364029350629
41	23.21615688132727	24.823810911367204	29.934007022127652	22.026025185177875
42	23.4194093325022	24.6862606736757	30.158082418914457	21.736247574907637
43	23.370415293459587	23.75507215004554	31.187493739823545	21.687018816671326
44	23.271938616236465	23.639025064608855	30.40797213681983	22.68106418233485
45	23.325732423280716	23.19694343875786	30.332996120091728	23.1443280178697
46	23.302974241867783	23.37991902571033	29.88448606244613	23.43262066997576
47	23.314825166055446	23.508808604173336	29.939199585514647	23.237166644256572
48	23.327988601652738	23.484795393602294	30.22534623356409	22.961869771180883
49	22.369879223562318	24.642947797157973	29.982919627980614	23.00425335129909
50	23.382764397160397	24.07481717410641	29.82569608970757	22.716722339025626
51	23.271205717529632	23.93836867956897	29.70620006917989	23.084225533721508
52	23.53933167972863	23.73613174817083	29.55119438777786	23.173342184322685
53	23.98045047786672	24.115342161425588	28.66463125207505	23.239576108632644
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4102.0
1	2714.0
2	1326.0
3	1052.5
4	779.0
5	765.5
6	752.0
7	816.0
8	1051.0
9	1222.0
10	1891.5
11	2561.0
12	3845.5
13	5130.0
14	6494.0
15	7858.0
16	11756.5
17	15655.0
18	21855.5
19	28056.0
20	43648.5
21	59241.0
22	550014.5
23	1040788.0
24	711757.0
25	323667.5
26	264609.0
27	261094.5
28	257580.0
29	272140.5
30	286701.0
31	307188.0
32	327675.0
33	364226.0
34	400777.0
35	452194.5
36	503612.0
37	563892.0
38	624172.0
39	687023.5
40	749875.0
41	828144.0
42	991015.0
43	1075617.0
44	1177476.0
45	1279335.0
46	1362063.5
47	1444792.0
48	1553254.0
49	1661716.0
50	1639545.5
51	1617375.0
52	1594510.5
53	1571646.0
54	1535743.5
55	1499841.0
56	1448611.5
57	1397382.0
58	1226329.5
59	916636.5
60	777996.0
61	676297.5
62	574599.0
63	495355.0
64	416111.0
65	339765.5
66	263420.0
67	213317.0
68	163214.0
69	130676.5
70	98139.0
71	73434.0
72	48729.0
73	37279.0
74	25829.0
75	20567.5
76	11807.0
77	8308.0
78	6353.0
79	4398.0
80	3562.5
81	2727.0
82	1944.5
83	1162.0
84	762.5
85	363.0
86	245.0
87	127.0
88	94.5
89	62.0
90	41.5
91	21.0
92	17.5
93	10.0
94	6.0
95	4.0
96	2.0
97	3.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.0876009E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.35055903752129
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.74094304067007	27.12954712451149
2	14.71836528763644	11.289150737986105
3	5.211187561306854	5.995558686764854
4	2.4509775566456415	3.7598543794311343
5	1.3918018366135594	2.668818925178944
6	0.9167907827476247	2.1095663423290882
7	0.6337238333440346	1.7012564298901294
8	0.4633196110284862	1.4214852876791488
9	0.36630566791757596	1.2643224428926498
>10	2.816681599861398	23.1491080298763
>50	0.22402107814127736	5.482078892954393
>100	0.057235081608116446	4.102094744058282
>500	0.0058309780993663005	1.5126316207378627
>1k	0.002428737790762102	1.8025646426267312
>5k	1.8076174149380235E-4	0.47111509683775776
>10k+	2.065848474214808E-4	6.140846616244963
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	823049	3.9425591357045304	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	99229	0.47532552797807287	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	53551	0.2565193375802818	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	45601	0.2184373459505598	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA	42829	0.20515894585023411	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	35627	0.1706600145650445	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC	28222	0.13518867519169972	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1017431540674274E-4	0.0	0.0	4.790187626380119E-6	1.9160750505520475E-5
2	1.3891544116502344E-4	0.0	0.0	4.790187626380119E-6	2.3950938131900596E-5
3	1.3891544116502344E-4	0.0	0.0	9.580375252760238E-6	3.3531313384660834E-5
4	1.6765656692330417E-4	0.0	0.0	9.580375252760238E-6	3.3531313384660834E-5
5	1.7244675454968427E-4	0.0	0.0	9.580375252760238E-6	5.7482251516561426E-5
6	1.9160750505520477E-4	0.0	0.0	1.4370562879140357E-5	6.227243914294155E-5
7	2.01187880307965E-4	0.0	0.0	1.4370562879140357E-5	6.706262676932167E-5
8	2.01187880307965E-4	0.0	0.0	1.4370562879140357E-5	6.706262676932167E-5
9	2.01187880307965E-4	0.0	0.0	1.9160750505520475E-5	7.66430020220819E-5
10	2.1076825556072524E-4	0.0	0.0	1.9160750505520475E-5	9.101356490122225E-5
11	2.1076825556072524E-4	0.0	0.0	1.9160750505520475E-5	1.3412525353864333E-4
12	2.1555844318710534E-4	0.0	0.0	1.9160750505520475E-5	1.6286637929692404E-4
13	2.251388184398656E-4	0.0	0.0	1.9160750505520475E-5	1.772369421760644E-4
14	2.3471919369262584E-4	0.0	0.0	1.9160750505520475E-5	1.8681731742882464E-4
15	2.4429956894538604E-4	0.0	0.0	2.3950938131900596E-5	1.9639769268158487E-4
16	2.6825050707728667E-4	0.0	0.0	2.3950938131900596E-5	2.1076825556072524E-4
17	2.778308823300469E-4	0.0	0.0	2.3950938131900596E-5	2.299290060662457E-4
18	3.065720080883276E-4	0.0	0.0	2.8741125758280713E-5	2.299290060662457E-4
19	3.257327585938481E-4	0.0	0.0	2.8741125758280713E-5	2.3471919369262584E-4
20	3.3531313384660834E-4	0.0	4.790187626380119E-6	3.3531313384660834E-5	2.3950938131900594E-4
21	3.736346348576493E-4	0.0	4.790187626380119E-6	3.3531313384660834E-5	2.3950938131900594E-4
22	3.8321501011040954E-4	0.0	4.790187626380119E-6	4.311168863742107E-5	2.490897565717662E-4
23	4.1674632349507037E-4	0.0	4.790187626380119E-6	4.311168863742107E-5	2.6346031945090657E-4
24	4.263266987478306E-4	0.0	4.790187626380119E-6	5.269206389018131E-5	2.8262106995642703E-4
25	4.4069726162697094E-4	0.0	4.790187626380119E-6	5.269206389018131E-5	2.8262106995642703E-4
26	4.646481997588715E-4	0.0	4.790187626380119E-6	5.269206389018131E-5	3.017818204619475E-4
27	4.8380895026439204E-4	0.0	4.790187626380119E-6	5.269206389018131E-5	3.065720080883276E-4
28	4.981795131435324E-4	0.0	4.790187626380119E-6	5.7482251516561426E-5	3.2094257096746797E-4
29	5.221304512754329E-4	0.0	4.790187626380119E-6	6.227243914294155E-5	3.257327585938481E-4
30	5.60451952286474E-4	0.0	9.580375252760238E-6	6.227243914294155E-5	3.496836967257487E-4
31	5.844028904183745E-4	0.0	1.4370562879140357E-5	6.227243914294155E-5	3.544738843521288E-4
32	6.514655171876962E-4	0.0	1.4370562879140357E-5	6.227243914294155E-5	3.592640719785089E-4
33	7.185281439570178E-4	0.0	1.4370562879140357E-5	6.706262676932167E-5	3.784248224840294E-4
34	8.095417088582401E-4	0.0	1.4370562879140357E-5	6.706262676932167E-5	3.8321501011040954E-4
35	8.718141480011817E-4	0.0	1.4370562879140357E-5	7.185281439570178E-5	3.9279538536316974E-4
36	9.29296399517743E-4	0.0	1.9160750505520475E-5	7.66430020220819E-5	3.9279538536316974E-4
37	9.963590262870648E-4	0.0	1.9160750505520475E-5	9.101356490122225E-5	4.119561358686902E-4
38	0.001049051090177246	0.0	1.9160750505520475E-5	1.005939401539825E-4	4.1674632349507037E-4
39	0.0010969529664410472	0.0	1.9160750505520475E-5	1.005939401539825E-4	4.1674632349507037E-4
40	0.0011831763437158893	0.0	1.9160750505520475E-5	1.1975469065950297E-4	4.1674632349507037E-4
41	0.001288560471496252	0.0	1.9160750505520475E-5	1.1975469065950297E-4	4.311168863742107E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	504075	0.0	46.19394	1
AGCAGTG	512445	0.0	45.40916	2
GTGGTAT	515495	0.0	45.141865	6
AGTGGTA	515165	0.0	45.108288	5
GTATCAA	516835	0.0	45.03892	9
TGGTATC	515420	0.0	45.00709	7
GGTATCA	517250	0.0	44.9937	8
TCAACGC	520375	0.0	44.9511	12
TATCAAC	518500	0.0	44.93236	10
CAACGCA	521495	0.0	44.921253	13
GCAGTGG	517765	0.0	44.910816	3
AACGCAG	523570	0.0	44.88865	14
ATCAACG	521595	0.0	44.871193	11
ACGCAGA	520880	0.0	44.868267	15
CAGTGGT	519415	0.0	44.72517	4
AGAGTAC	514955	0.0	44.723724	19
CGCAGAG	522835	0.0	44.68746	16
CAGAGTA	519300	0.0	44.612896	18
GAGTACT	150565	0.0	44.519905	20
GCAGAGT	523595	0.0	44.444862	17
>>END_MODULE