FastQCFastQC Report
Thu 26 May 2016
SRR805178_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805178_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20593481
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4485202.177970785997763No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC353280.17154943353190263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2360450.045.0795441
TCAACGC2421600.043.85774212
AGCAGTG2439100.043.8195882
CAACGCA2412450.043.79419713
GTATCAA2422500.043.783249
AACGCAG2432900.043.7361414
GTGGTAT2440450.043.722166
GGTATCA2426250.043.7146038
ACGCAGA2424200.043.6924415
AGTGGTA2437900.043.6753585
ATCAACG2431700.043.6572211
TATCAAC2433950.043.5743810
TGGTATC2434450.043.5306747
AGAGTAC2412400.043.3586919
CGCAGAG2443800.043.3343216
GCAGTGG2472950.043.2007753
CAGTGGT2477600.043.0381244
CAGAGTA2435250.042.88141318
GAGTACT768650.042.6983720
GCAGAGT2462700.042.6334117