##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805178_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20593481
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.224726553029086	37.0	37.0	37.0	35.0	37.0
2	36.344486296415845	37.0	37.0	37.0	35.0	37.0
3	38.19016503329379	39.0	39.0	39.0	37.0	39.0
4	38.05661597473492	39.0	39.0	39.0	37.0	39.0
5	37.92331704387422	39.0	39.0	39.0	35.0	39.0
6	37.977242846898974	39.0	39.0	39.0	35.0	39.0
7	37.951613668422546	39.0	38.0	39.0	35.0	39.0
8	38.949280600011235	40.0	40.0	40.0	37.0	40.0
9	38.95565693823205	40.0	40.0	40.0	37.0	40.0
10	38.764091170404846	40.0	39.0	40.0	36.0	40.0
11	38.80780578086823	40.0	39.0	40.0	36.0	40.0
12	38.92721497642871	40.0	40.0	40.0	37.0	40.0
13	38.88196730800393	40.0	40.0	40.0	37.0	40.0
14	39.040420121299555	40.0	40.0	40.0	37.0	40.0
15	39.06472980454349	40.0	40.0	40.0	37.0	40.0
16	39.04212604950081	40.0	40.0	40.0	37.0	40.0
17	39.023405028028044	40.0	40.0	40.0	37.0	40.0
18	38.899391462764356	40.0	40.0	40.0	37.0	40.0
19	38.804853827286415	40.0	40.0	40.0	37.0	40.0
20	38.892752322931706	40.0	40.0	40.0	37.0	40.0
21	38.77910247422473	40.0	40.0	40.0	37.0	40.0
22	38.8528343508317	40.0	40.0	40.0	37.0	40.0
23	38.41100491946942	40.0	38.0	40.0	36.0	40.0
24	38.63341200062292	40.0	39.0	40.0	36.0	40.0
25	38.739462405603014	40.0	39.0	40.0	36.0	40.0
26	38.71808675764918	40.0	39.0	40.0	36.0	40.0
27	38.19346034796157	40.0	39.0	40.0	35.0	40.0
28	38.43940992783104	40.0	39.0	40.0	35.0	40.0
29	38.4379287309416	40.0	39.0	40.0	35.0	40.0
30	38.45118831537029	40.0	39.0	40.0	35.0	40.0
31	38.293314520260076	40.0	39.0	40.0	35.0	40.0
32	38.32076335224725	40.0	39.0	40.0	35.0	40.0
33	38.30621044591733	40.0	38.0	40.0	35.0	40.0
34	38.317647220496625	40.0	38.0	40.0	35.0	40.0
35	38.1204627328425	40.0	38.0	40.0	35.0	40.0
36	38.044579544371345	40.0	38.0	40.0	35.0	40.0
37	38.170911076179884	40.0	38.0	40.0	35.0	40.0
38	38.164494628178694	40.0	38.0	40.0	35.0	40.0
39	38.133453154422995	40.0	38.0	40.0	35.0	40.0
40	38.04420083229251	40.0	38.0	40.0	35.0	40.0
41	37.96955201502845	40.0	38.0	40.0	34.0	40.0
42	37.84256911204084	40.0	38.0	40.0	34.0	40.0
43	37.8413959252445	40.0	38.0	40.0	34.0	40.0
44	37.835581900893786	40.0	37.0	40.0	34.0	40.0
45	37.70768812713111	40.0	37.0	40.0	34.0	40.0
46	37.610070293604075	40.0	37.0	40.0	34.0	40.0
47	37.4460625670813	40.0	37.0	40.0	33.0	40.0
48	37.37577522712163	40.0	37.0	40.0	33.0	40.0
49	37.23544348816016	40.0	36.0	40.0	33.0	40.0
50	37.18039266892275	40.0	36.0	40.0	33.0	40.0
51	37.020762007161395	39.0	36.0	40.0	33.0	40.0
52	36.869184282152204	39.0	36.0	40.0	32.0	40.0
53	36.627255683485465	39.0	35.0	40.0	32.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	12.0
13	42.0
14	153.0
15	454.0
16	1076.0
17	1975.0
18	3493.0
19	5614.0
20	8778.0
21	13472.0
22	18954.0
23	25631.0
24	34488.0
25	44404.0
26	56601.0
27	71900.0
28	91095.0
29	114840.0
30	144146.0
31	183106.0
32	235365.0
33	309891.0
34	432540.0
35	650482.0
36	1048876.0
37	1938016.0
38	3671957.0
39	1.1486117E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.471561121696716	34.88947303275245	28.554240052956565	21.08472579259427
2	23.966603800493953	37.34186561271501	18.589436142437503	20.10209444435353
3	34.46314879937005	24.051980332999552	20.247009235592564	21.237861632037827
4	22.242844713819874	22.69986798249407	21.34369609489527	33.71359120879078
5	22.2477054753395	34.042039808617105	22.778572500686018	20.931682215357377
6	33.39459705719495	23.130567386834695	21.605312865755916	21.869522690214442
7	22.394062470545897	22.74174045660372	33.939886122215086	20.9243109506353
8	32.54846035985854	23.882081907376417	22.637984321349073	20.931473411415972
9	32.1817180883601	23.54350388843926	23.15171485578373	21.123063167416912
10	21.0394153373099	23.67448223056607	34.10314652486387	21.18295590726017
11	21.22425538450736	34.45265033143256	23.14838856043813	21.174705723621955
12	20.901065730461013	23.642059348781295	34.14195977843668	21.31491514232101
13	21.20841056448883	23.66504720595804	23.1673654395777	31.95917678997543
14	21.176473273265454	34.640734123580174	23.070373580843377	21.112419022310995
15	21.099201247229647	34.420334279571286	23.09154532932048	21.38891914387859
16	21.105222570191025	23.364660884675107	23.28196481206844	32.24815173306543
17	32.2824441385116	23.262254691181155	23.20204631747299	21.253254852834253
18	21.4184090586725	23.354934505730235	23.135331030242046	32.09132540535522
19	21.529181977539398	34.3137228718156	23.144217337515695	21.01287781312931
20	32.38654989897046	23.32643033977597	23.249216584607527	21.037803176646047
21	21.508724047187556	34.14661659192052	23.213588805117503	21.13107055577442
22	32.094947910943276	23.229652140888664	23.44665285096774	21.228747097200323
23	21.441955344994856	23.329445857162273	34.040757849535005	21.187840948307866
24	21.23348160517399	34.137006754710384	23.21944988319362	21.41006175692201
25	21.195110239012045	23.332252570607174	23.340978632995558	32.13165855738522
26	21.51097718739246	30.648490170263106	26.64699086084572	21.193541781498716
27	21.87543718325231	23.26390569908992	33.65453368471314	21.20612343294463
28	29.057821744657936	23.13190761678417	26.641950430818373	21.168320207739526
29	29.28046987296611	23.18092312805203	26.439051270642395	21.099555728339467
30	28.926634598589718	23.203804155305264	26.532945061594976	21.336616184510042
31	26.116415189836044	24.37421337363994	27.200447559108632	22.30892387741538
32	23.96029597910135	25.7162351522795	27.824654802167736	22.498814066451416
33	23.62217927119752	25.08642905004744	28.207994559054878	23.083397119700162
34	23.223742503756405	24.873618986513257	28.656490857470867	23.24614765225947
35	23.492817945640176	24.981123880901922	28.412331067292605	23.113727106165296
36	23.362781649202482	25.097495658941778	28.425359462055006	23.114363229800734
37	23.355643467949882	24.910164532164327	28.644414220208812	23.089777779676975
38	23.668412348548554	24.93008831289863	28.394849807082156	23.006649531470664
39	23.61412332378387	24.832358356510976	28.423543353355363	23.12997496634979
40	23.37226037696104	24.96677953571812	28.339774125608002	23.321185961712835
41	23.547087546782404	25.30753300037036	28.118145737478766	23.02723371536847
42	23.771585775129516	25.180419959112303	28.14753367825478	22.900460587503396
43	23.581239130965763	24.975369632749313	28.53049467450403	22.912896561780887
44	23.55711013596973	24.94050423044069	28.205552038531028	23.29683359505855
45	23.536958127671568	24.777360369526647	28.201113740799823	23.484567762001966
46	23.546101797942757	24.864208241433296	28.18453082312796	23.405159137495986
47	23.84208381283378	24.94298074230384	27.929042205152204	23.28589323971018
48	23.789159297546636	24.904517113935228	28.010645698995717	23.295677889522416
49	23.35674090261865	25.240706027310296	28.02648080720302	23.376072262868043
50	23.646342257532858	25.109266374150153	27.976727198281825	23.267664170035168
51	23.566083849544427	25.028415545676808	27.917766792316463	23.487733812462302
52	23.5928933044394	25.096573036875114	27.83089464088174	23.47963901780374
53	23.84363284672465	25.24025442808819	27.373026444630703	23.543086280556455
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2764.0
1	1809.5
2	855.0
3	736.5
4	618.0
5	634.0
6	650.0
7	791.5
8	1221.5
9	1510.0
10	2384.5
11	3259.0
12	4839.0
13	6419.0
14	8230.5
15	10042.0
16	14676.0
17	19310.0
18	27240.0
19	35170.0
20	50440.5
21	65711.0
22	340673.5
23	615636.0
24	426449.0
25	238800.0
26	240338.0
27	259317.5
28	278297.0
29	305565.5
30	332834.0
31	362540.0
32	392246.0
33	438141.5
34	484037.0
35	543973.0
36	603909.0
37	669322.5
38	734736.0
39	799842.0
40	864948.0
41	942623.5
42	1095101.5
43	1169904.0
44	1249224.5
45	1328545.0
46	1386992.5
47	1445440.0
48	1506784.5
49	1568129.0
50	1566383.0
51	1564637.0
52	1537812.5
53	1510988.0
54	1466300.5
55	1421613.0
56	1331891.5
57	1242170.0
58	1126507.5
59	893479.5
60	776114.0
61	679016.5
62	581919.0
63	497495.5
64	413072.0
65	338693.0
66	264314.0
67	213183.0
68	162052.0
69	126698.0
70	91344.0
71	67979.0
72	44614.0
73	33687.5
74	22761.0
75	17401.5
76	8924.0
77	5806.0
78	4219.0
79	2632.0
80	2076.0
81	1520.0
82	1124.0
83	728.0
84	490.5
85	253.0
86	183.0
87	113.0
88	84.0
89	55.0
90	43.0
91	31.0
92	28.0
93	20.0
94	15.0
95	13.0
96	11.0
97	7.0
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.0593481E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.09686156771519
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.20045447670327	30.95619723049876
2	15.292531656784819	13.487053029782848
3	5.506565647211318	7.284667891758402
4	2.5285914609178417	4.460117904536031
5	1.416497385841398	3.123154456723929
6	0.8895887462252351	2.35368430766949
7	0.6191166998226476	1.911077238443798
8	0.44212685242087413	1.5597125285258344
9	0.3510450729924759	1.3931987388999914
>10	2.582527521032384	22.55432792421039
>50	0.1306187525961895	3.8219423067585536
>100	0.03741712242665247	3.055865984135249
>500	0.002110740522436788	0.62284368703824
>1k	6.751979695476585E-4	0.5449187854091996
>5k	7.738881095776344E-5	0.27031233778544367
>10k+	5.5277722112685734E-5	2.6009256478239444
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	448520	2.177970785997763	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	35328	0.17154943353190263	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.60244885262477E-4	0.0	0.0	0.0	4.855905614014454E-6
2	2.2822756385867935E-4	0.0	0.0	0.0	9.711811228028909E-6
3	2.3793937508670827E-4	0.0	0.0	0.0	9.711811228028909E-6
4	2.476511863147372E-4	0.0	0.0	0.0	1.9423622456057818E-5
5	2.573629975427661E-4	0.0	0.0	0.0	1.9423622456057818E-5
6	2.622189031567805E-4	0.0	0.0	0.0	2.9135433684086725E-5
7	2.719307143848094E-4	0.0	0.0	0.0	2.9135433684086725E-5
8	2.767866199988239E-4	0.0	0.0	4.855905614014454E-6	2.9135433684086725E-5
9	2.767866199988239E-4	0.0	0.0	4.855905614014454E-6	3.399133929810118E-5
10	2.8649843122685283E-4	0.0	0.0	4.855905614014454E-6	4.855905614014454E-5
11	2.913543368408673E-4	0.0	0.0	4.855905614014454E-6	6.31267729821879E-5
12	2.9621024245488173E-4	0.0	0.0	4.855905614014454E-6	6.798267859620236E-5
13	3.0592205368291063E-4	0.0	0.0	4.855905614014454E-6	7.769448982423127E-5
14	3.107779592969251E-4	0.0	4.855905614014454E-6	4.855905614014454E-6	8.740630105226018E-5
15	3.1563386491093953E-4	0.0	4.855905614014454E-6	4.855905614014454E-6	9.226220666627463E-5
16	3.20489770524954E-4	0.0	4.855905614014454E-6	1.4567716842043362E-5	9.711811228028908E-5
17	3.544811098230552E-4	0.0	4.855905614014454E-6	1.4567716842043362E-5	1.0197401789430355E-4
18	3.641929210510841E-4	0.0	4.855905614014454E-6	1.4567716842043362E-5	1.0197401789430355E-4
19	3.73904732279113E-4	0.0	4.855905614014454E-6	1.4567716842043362E-5	1.06829923508318E-4
20	3.9818426034918524E-4	0.0	4.855905614014454E-6	1.4567716842043362E-5	1.165417347363469E-4
21	4.3217559964728644E-4	0.0	4.855905614014454E-6	1.4567716842043362E-5	1.165417347363469E-4
22	4.4188741087531534E-4	0.0	4.855905614014454E-6	1.9423622456057818E-5	1.2139764035036136E-4
23	4.5159922210334424E-4	0.0	4.855905614014454E-6	1.9423622456057818E-5	1.262535459643758E-4
24	5.098700894715177E-4	0.0	9.711811228028909E-6	1.9423622456057818E-5	1.408212628064192E-4
25	5.147259950855322E-4	0.0	9.711811228028909E-6	1.9423622456057818E-5	1.4567716842043364E-4
26	5.632850512256768E-4	0.0	9.711811228028909E-6	1.9423622456057818E-5	1.4567716842043364E-4
27	6.069882017518068E-4	0.0	9.711811228028909E-6	1.9423622456057818E-5	1.505330740344481E-4
28	6.40979541049908E-4	0.0	9.711811228028909E-6	1.9423622456057818E-5	1.699566964905059E-4
29	6.652590691199802E-4	0.0	9.711811228028909E-6	2.427952807007227E-5	1.699566964905059E-4
30	6.94394502804067E-4	0.0	9.711811228028909E-6	2.427952807007227E-5	1.7966850771853482E-4
31	7.186740308741393E-4	0.0	9.711811228028909E-6	2.9135433684086725E-5	1.7966850771853482E-4
32	7.672330870142838E-4	0.0	9.711811228028909E-6	2.9135433684086725E-5	1.7966850771853482E-4
33	8.352157656104862E-4	0.0	9.711811228028909E-6	2.9135433684086725E-5	1.9423622456057817E-4
34	8.789189161366162E-4	0.0	9.711811228028909E-6	2.9135433684086725E-5	1.9909213017459262E-4
35	9.031984442066885E-4	0.0	9.711811228028909E-6	3.399133929810118E-5	1.9909213017459262E-4
36	9.663252171888765E-4	0.0	9.711811228028909E-6	3.399133929810118E-5	2.2822756385867935E-4
37	0.0010003165564869776	0.0	9.711811228028909E-6	3.399133929810118E-5	2.2822756385867935E-4
38	0.00106829923508318	0.0	9.711811228028909E-6	4.370315052613009E-5	2.330834694726938E-4
39	0.0011265701024513535	0.0	9.711811228028909E-6	4.370315052613009E-5	2.330834694726938E-4
40	0.0011702732529774835	0.0	9.711811228028909E-6	4.855905614014454E-5	2.330834694726938E-4
41	0.001272247270871787	0.0	9.711811228028909E-6	4.855905614014454E-5	2.330834694726938E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	236045	0.0	45.079544	1
TCAACGC	242160	0.0	43.857742	12
AGCAGTG	243910	0.0	43.819588	2
CAACGCA	241245	0.0	43.794197	13
GTATCAA	242250	0.0	43.78324	9
AACGCAG	243290	0.0	43.73614	14
GTGGTAT	244045	0.0	43.72216	6
GGTATCA	242625	0.0	43.714603	8
ACGCAGA	242420	0.0	43.69244	15
AGTGGTA	243790	0.0	43.675358	5
ATCAACG	243170	0.0	43.65722	11
TATCAAC	243395	0.0	43.57438	10
TGGTATC	243445	0.0	43.530674	7
AGAGTAC	241240	0.0	43.35869	19
CGCAGAG	244380	0.0	43.33432	16
GCAGTGG	247295	0.0	43.200775	3
CAGTGGT	247760	0.0	43.038124	4
CAGAGTA	243525	0.0	42.881413	18
GAGTACT	76865	0.0	42.69837	20
GCAGAGT	246270	0.0	42.63341	17
>>END_MODULE