FastQCFastQC Report
Thu 26 May 2016
SRR805177_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805177_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19114379
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2676761.4003907738776133No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC236140.12354050319918841No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1397800.044.4411931
AGCAGTG1492400.041.7737542
TCAACGC1540950.040.18309812
AGTGGTA1547550.040.1089135
CAACGCA1535850.040.10231813
GTATCAA1550500.039.953799
AACGCAG1552400.039.92608314
GTGGTAT1560000.039.912346
GCAGTGG1562800.039.8874443
GGTATCA1553450.039.8824548
ATCAACG1556300.039.87736511
ACGCAGA1549700.039.8227715
TGGTATC1556550.039.757737
TATCAAC1562700.039.6508910
AGAGTAC1546450.039.44449619
CGCAGAG1568550.039.33670816
CAGTGGT1587000.039.1873974
GAGTACT499600.039.003620
CAGAGTA1567900.038.80894518
GCAGAGT1595100.038.34900317
Produced by FastQC (version 0.11.5)