##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805177_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19114379
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.164448502355214	37.0	37.0	37.0	35.0	37.0
2	36.28932171952853	37.0	37.0	37.0	35.0	37.0
3	38.1606676314203	39.0	39.0	39.0	37.0	39.0
4	38.06680007757511	39.0	39.0	39.0	37.0	39.0
5	37.96795893813762	39.0	39.0	39.0	35.0	39.0
6	38.01112105185316	39.0	39.0	39.0	35.0	39.0
7	38.014339571272494	39.0	39.0	39.0	35.0	39.0
8	38.98054919806707	40.0	40.0	40.0	37.0	40.0
9	38.95853231747681	40.0	40.0	40.0	37.0	40.0
10	38.87952818137592	40.0	39.0	40.0	37.0	40.0
11	38.84855301864633	40.0	39.0	40.0	37.0	40.0
12	38.94210839912717	40.0	40.0	40.0	37.0	40.0
13	38.88230891518893	40.0	40.0	40.0	37.0	40.0
14	39.02870603329567	40.0	40.0	40.0	37.0	40.0
15	39.05892652855738	40.0	40.0	40.0	37.0	40.0
16	39.040170020694895	40.0	40.0	40.0	37.0	40.0
17	39.036220899460034	40.0	40.0	40.0	37.0	40.0
18	38.90704735947739	40.0	40.0	40.0	37.0	40.0
19	38.80782498871661	40.0	40.0	40.0	37.0	40.0
20	38.89115387949564	40.0	40.0	40.0	37.0	40.0
21	38.783298793018595	40.0	40.0	40.0	37.0	40.0
22	38.84003236516342	40.0	40.0	40.0	37.0	40.0
23	38.530630160676424	40.0	39.0	40.0	36.0	40.0
24	38.686760684194866	40.0	39.0	40.0	36.0	40.0
25	38.7568497516974	40.0	39.0	40.0	36.0	40.0
26	38.72938205316532	40.0	39.0	40.0	36.0	40.0
27	38.19852196087563	40.0	39.0	40.0	35.0	40.0
28	38.43706970548193	40.0	39.0	40.0	35.0	40.0
29	38.42729512687804	40.0	39.0	40.0	35.0	40.0
30	38.44416980535962	40.0	39.0	40.0	35.0	40.0
31	38.30767528466397	40.0	39.0	40.0	35.0	40.0
32	38.33698086660309	40.0	39.0	40.0	35.0	40.0
33	38.33142227639203	40.0	39.0	40.0	35.0	40.0
34	38.33129645488352	40.0	39.0	40.0	35.0	40.0
35	38.11955695761814	40.0	38.0	40.0	35.0	40.0
36	38.05042774342813	40.0	38.0	40.0	35.0	40.0
37	38.19767343736357	40.0	38.0	40.0	35.0	40.0
38	38.21490658943197	40.0	38.0	40.0	35.0	40.0
39	38.183923474573774	40.0	38.0	40.0	35.0	40.0
40	38.09923115995555	40.0	38.0	40.0	35.0	40.0
41	38.02592367766695	40.0	38.0	40.0	35.0	40.0
42	37.89514637122137	40.0	38.0	40.0	34.0	40.0
43	37.90164707940551	40.0	38.0	40.0	34.0	40.0
44	37.9053230031695	40.0	38.0	40.0	34.0	40.0
45	37.77567714860106	40.0	38.0	40.0	34.0	40.0
46	37.683571566724716	40.0	37.0	40.0	34.0	40.0
47	37.516615894243806	40.0	37.0	40.0	33.0	40.0
48	37.46229568849713	40.0	37.0	40.0	33.0	40.0
49	37.328638508214155	40.0	37.0	40.0	33.0	40.0
50	37.29654769323136	40.0	36.0	40.0	33.0	40.0
51	37.15801957259506	40.0	36.0	40.0	33.0	40.0
52	37.04452961825231	39.0	36.0	40.0	33.0	40.0
53	36.85163007388312	39.0	36.0	40.0	32.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	9.0
13	58.0
14	163.0
15	504.0
16	1140.0
17	2123.0
18	3589.0
19	5602.0
20	8709.0
21	12732.0
22	18141.0
23	24642.0
24	31795.0
25	41509.0
26	52792.0
27	67417.0
28	84410.0
29	106688.0
30	134213.0
31	169113.0
32	215317.0
33	282650.0
34	389415.0
35	578925.0
36	924542.0
37	1687110.0
38	3313535.0
39	1.0957536E7
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.693767817411175	29.921861442634363	22.230887019662006	26.15348372029246
2	23.221821645369698	30.30501278644731	22.201730958667294	24.271434609515698
3	26.162665289832333	23.68794194150906	26.38215450263909	23.767238266019525
4	21.58178405900605	23.85561152679875	26.301660127174415	28.260944287020784
5	24.103519136038894	30.464667463170002	25.377999463126688	20.053813937664415
6	29.088049368488505	24.17902773613519	25.606455747267542	21.12646714810876
7	23.34883074150617	24.544804725280375	31.815608553121187	20.290755980092264
8	28.90662573971145	24.609557025106596	24.92947848318797	21.554338751993985
9	28.061225530790196	24.309923958293385	25.974911348153135	21.653939162763276
10	21.655252310315706	24.476515820890647	31.873894516792827	21.994337352000816
11	21.867218390929676	31.293352507031486	24.80974139939362	22.02968770264522
12	21.58247987025893	24.740264907376798	31.673176512823147	22.004078709541126
13	22.018868622412477	24.89760195714441	24.384443773977697	28.699085646465416
14	21.945667185944153	31.757615562608656	24.508319103644435	21.788398147802763
15	21.94520679955127	31.320196172734672	24.723936885420134	22.010660142293926
16	21.8662871548168	24.58384863039495	24.591962940569505	28.957901274218745
17	28.80816583159725	24.60630816203864	24.699159726821364	21.886366279542745
18	22.012187788052128	24.586658033724245	24.825258513499183	28.57589566472445
19	22.395809981585067	31.387841582507075	24.5286545798846	21.68769385602326
20	29.05364071728409	24.380901937750632	24.750267848094882	21.815189496870392
21	22.092687395180352	31.209080870479756	24.875759761800264	21.822471972539624
22	28.77486106140304	24.410204485324897	24.714169369562043	22.100765083710016
23	22.086498337194215	24.63085512744097	31.203326040568726	22.07932049479609
24	21.80851912583715	31.38648134998265	24.673268223885277	22.131731300294923
25	22.12790172257231	24.381639602311957	24.68840342655129	28.802055248564447
26	22.465966589864102	28.941662190542523	26.814138194078918	21.778233025514457
27	22.753896425303694	24.268829241065067	31.06823402423903	21.90904030939221
28	27.217776732375143	24.180325188696948	26.658538056611725	21.943360022316185
29	27.22693737526079	24.326733293297156	26.62352776409843	21.82280156734362
30	26.713376354000307	24.344269829535136	26.875845665715847	22.066508150748714
31	25.27327725373657	24.987267438821842	27.068841734277633	22.67061357316395
32	23.826141565990714	25.865234753375983	27.543908175096877	22.764715505536433
33	23.453008857886516	25.508806747004442	27.934315836261277	23.103868558847765
34	23.36438970892018	25.3082718512592	28.04675474939573	23.280583690424887
35	23.535470338848047	25.464452703381053	27.903108963152818	23.096967994618083
36	23.421106173525178	25.480608080440387	28.011618896957103	23.086666849077336
37	23.516903164889634	25.338871851395222	27.931558749567536	23.2126662341476
38	23.612449036403433	25.472797206752045	27.868867725182177	23.04588603166234
39	23.563893966944992	25.40000384004105	27.927080445564044	23.10902174744992
40	23.60746849269861	25.421485050599863	27.644690941829708	23.326355514871814
41	23.619187419062897	25.758545438489005	27.593577588892632	23.028689553555466
42	23.700424690752445	25.635303140112477	27.703777350025337	22.96049481910974
43	23.56537452773119	25.40720784075695	27.860612160091623	23.166805471420233
44	23.551735580842045	25.425665149780695	27.726147943388586	23.29645132598867
45	23.554654849106004	25.295328715622933	27.818308928581985	23.331707506689074
46	23.70127222024843	25.312472877094255	27.551603952186987	23.434650950470324
47	23.714843155511357	25.440533537605383	27.51806898879634	23.326554318086924
48	23.57283487996131	25.336972757524585	27.75805585941348	23.332136503100624
49	23.531645992788988	25.625263577749507	27.461551327406454	23.381539102055054
50	23.627871980565	25.602877289395593	27.50611986923562	23.263130860803795
51	23.502400993513834	25.503297805280518	27.557374477088686	23.43692672411696
52	23.671404653010175	25.46628901728903	27.353847069789712	23.508459259911085
53	23.727979862699176	25.697287889917845	27.098724996506558	23.47600725087642
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3667.0
1	2609.5
2	1552.0
3	1339.5
4	1127.0
5	1167.0
6	1207.0
7	1377.5
8	1917.0
9	2286.0
10	3326.0
11	4366.0
12	6460.0
13	8554.0
14	10715.5
15	12877.0
16	18272.5
17	23668.0
18	33003.5
19	42339.0
20	58818.5
21	75298.0
22	254106.5
23	432915.0
24	325805.0
25	230898.0
26	243101.0
27	268694.0
28	294287.0
29	324467.5
30	354648.0
31	385742.0
32	416836.0
33	461694.0
34	506552.0
35	564845.5
36	623139.0
37	679517.5
38	735896.0
39	794630.5
40	853365.0
41	918075.5
42	1044646.0
43	1106506.0
44	1170528.5
45	1234551.0
46	1278637.5
47	1322724.0
48	1357685.5
49	1392647.0
50	1390713.5
51	1388780.0
52	1360908.5
53	1333037.0
54	1293968.0
55	1254899.0
56	1187819.0
57	1120739.0
58	1017495.5
59	809499.5
60	704747.0
61	619508.5
62	534270.0
63	458753.5
64	383237.0
65	315958.0
66	248679.0
67	201350.0
68	154021.0
69	121527.0
70	89033.0
71	66939.0
72	44845.0
73	33488.0
74	22131.0
75	17073.0
76	9039.0
77	6063.0
78	4456.0
79	2849.0
80	2378.0
81	1907.0
82	1475.5
83	1044.0
84	683.0
85	322.0
86	242.5
87	163.0
88	122.5
89	82.0
90	68.5
91	55.0
92	46.5
93	28.0
94	18.0
95	13.0
96	8.0
97	7.5
98	7.0
99	4.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.9114379E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.635112669144284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.80025570977286	32.3097764631971
2	14.902401335004727	13.601455279274937
3	5.252757801055592	7.191305822246956
4	2.4510692516476835	4.474192858352688
5	1.4652035122844382	3.3432363683163135
6	0.9250863771280173	2.5329852629356546
7	0.6533705614615243	2.087164743089911
8	0.5306066382088125	1.937143497412403
9	0.3975626758146031	1.6328535753461306
>10	2.4515493915213806	20.87856939288487
>50	0.13083381106121708	3.954391775036023
>100	0.036389366253277836	3.02915689230834
>500	0.0023028916968650197	0.696414191741066
>1k	4.8406221097302285E-4	0.36591226048672104
>5k	5.755221741496135E-5	0.1892900396172158
>10k+	6.906266089794868E-5	1.7761515777534795
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	267676	1.4003907738776133	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	23614	0.12354050319918841	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.3945973866061776E-4	0.0	0.0	5.231663555483544E-6	1.0463327110967089E-5
2	4.551547293270684E-4	0.0	0.0	1.0463327110967089E-5	3.1389981332901266E-5
3	4.7084971999351903E-4	0.0	0.0	1.5694990666450633E-5	3.662164488838481E-5
4	4.8654471065996967E-4	0.0	0.0	1.5694990666450633E-5	4.70849719993519E-5
5	9.99247739097357E-4	0.0	0.0	1.5694990666450633E-5	7.324328977676963E-5
6	0.0010201743933192912	0.0	0.0	2.0926654221934177E-5	8.370661688773671E-5
7	0.0010411010475412253	0.0	0.0	2.0926654221934177E-5	9.41699439987038E-5
8	0.001056796038207676	0.0	0.0	2.0926654221934177E-5	9.41699439987038E-5
9	0.0010724910288741267	0.0	0.0	2.615831777741772E-5	9.940160755418734E-5
10	0.0010829543559850937	0.0	0.0	2.615831777741772E-5	1.0463327110967089E-4
11	0.001182355963539281	0.0	0.0	3.662164488838481E-5	1.2555992533160506E-4
12	0.0012137459448721822	0.0	0.0	5.231663555483544E-5	1.3602325244257217E-4
13	0.0012922208982044355	5.231663555483544E-6	0.0	5.754829911031899E-5	1.3602325244257217E-4
14	0.0013236108795373369	5.231663555483544E-6	0.0	6.801162622128609E-5	1.4648657955353925E-4
15	0.0013602325244257217	5.231663555483544E-6	0.0	7.847495333225317E-5	1.621815702199899E-4
16	0.0013759275150921721	5.231663555483544E-6	0.0	9.41699439987038E-5	1.8310822444192405E-4
17	0.0013968541693141065	5.231663555483544E-6	0.0	9.940160755418734E-5	1.9357155155289116E-4
18	0.0014230124870915242	5.231663555483544E-6	0.0	9.940160755418734E-5	2.0403487866385824E-4
19	0.0014544024684244254	5.231663555483544E-6	0.0	9.940160755418734E-5	2.0926654221934177E-4
20	0.0014857924497573268	5.231663555483544E-6	0.0	1.0986493466515444E-4	2.1449820577482533E-4
21	0.0015014874404237772	5.231663555483544E-6	0.0	1.0986493466515444E-4	2.4065652355224304E-4
22	0.0015067191039792609	5.231663555483544E-6	0.0	1.0986493466515444E-4	2.6681484132966076E-4
23	0.001527645758201195	5.231663555483544E-6	0.0	1.1509659822063798E-4	2.929731591070785E-4
24	0.0015538040759786127	5.231663555483544E-6	0.0	1.2032826177612152E-4	2.929731591070785E-4
25	0.001585194057311514	5.231663555483544E-6	0.0	1.2032826177612152E-4	2.9820482266256203E-4
26	0.0015956573844224812	5.231663555483544E-6	0.0	1.2032826177612152E-4	3.0343648621804556E-4
27	0.001621815702199899	5.231663555483544E-6	0.0	1.2555992533160506E-4	3.0343648621804556E-4
28	0.001642742356421833	5.231663555483544E-6	0.0	1.2555992533160506E-4	3.0866814977352914E-4
29	0.0016532056835328	5.231663555483544E-6	0.0	1.2555992533160506E-4	3.3482646755094683E-4
30	0.0016793640013102178	5.231663555483544E-6	0.0	1.3079158888708862E-4	3.400581311064304E-4
31	0.0017055223190876356	5.231663555483544E-6	0.0	1.3602325244257217E-4	3.5052145821739747E-4
32	0.0017369123004205367	5.231663555483544E-6	0.0	1.5171824310902278E-4	3.714481124393317E-4
33	0.0017839972724198888	5.231663555483544E-6	0.0	1.6741323377547342E-4	3.871431031057823E-4
34	0.0018310822444192406	5.231663555483544E-6	0.0	1.7264489733095697E-4	3.9237476666126585E-4
35	0.0018624722257521418	5.231663555483544E-6	0.0	1.8310822444192405E-4	3.976064302167494E-4
36	0.0018938622070850432	5.231663555483544E-6	0.0	1.8310822444192405E-4	4.1853308443868354E-4
37	0.0019461788426398787	5.231663555483544E-6	0.0	1.8310822444192405E-4	4.237647479941671E-4
38	0.001961873833306329	5.231663555483544E-6	0.0	1.8310822444192405E-4	4.446914022161013E-4
39	0.002003727141750198	1.0463327110967089E-5	0.0	1.988032151083747E-4	4.446914022161013E-4
40	0.002066507104416	1.0463327110967089E-5	0.0	2.0403487866385824E-4	4.551547293270684E-4
41	0.002092665422193418	1.0463327110967089E-5	0.0	2.0926654221934177E-4	4.917763742154532E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	139780	0.0	44.441193	1
AGCAGTG	149240	0.0	41.773754	2
TCAACGC	154095	0.0	40.183098	12
AGTGGTA	154755	0.0	40.108913	5
CAACGCA	153585	0.0	40.102318	13
GTATCAA	155050	0.0	39.95379	9
AACGCAG	155240	0.0	39.926083	14
GTGGTAT	156000	0.0	39.91234	6
GCAGTGG	156280	0.0	39.887444	3
GGTATCA	155345	0.0	39.882454	8
ATCAACG	155630	0.0	39.877365	11
ACGCAGA	154970	0.0	39.82277	15
TGGTATC	155655	0.0	39.75773	7
TATCAAC	156270	0.0	39.65089	10
AGAGTAC	154645	0.0	39.444496	19
CGCAGAG	156855	0.0	39.336708	16
CAGTGGT	158700	0.0	39.187397	4
GAGTACT	49960	0.0	39.0036	20
CAGAGTA	156790	0.0	38.808945	18
GCAGAGT	159510	0.0	38.349003	17
>>END_MODULE