FastQCFastQC Report
Thu 26 May 2016
SRR805176_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805176_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12773227
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2685352.102327000060361No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC187850.1470654205080674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1203400.044.974951
AGCAGTG1291400.042.0230372
GTGGTAT1296950.041.638466
AGTGGTA1296700.041.6066175
TCAACGC1299750.041.53429812
GGTATCA1295300.041.4665348
CAACGCA1296300.041.42366813
GTATCAA1296300.041.4073529
TGGTATC1298300.041.3489957
AACGCAG1308450.041.3120114
ACGCAGA1303700.041.30209715
ATCAACG1304100.041.23537411
TATCAAC1304400.041.14481710
GCAGTGG1318750.041.128343
CAGTGGT1315700.041.1040154
CGCAGAG1315550.040.92470616
AGAGTAC1299400.040.80760619
GAGTACT481450.040.44957720
CAGAGTA1313600.040.4237218
GCAGAGT1331600.040.1049517
Produced by FastQC (version 0.11.5)