##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805176_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12773227
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.598037363620016	37.0	35.0	37.0	35.0	37.0
2	35.909789515210214	37.0	36.0	37.0	35.0	37.0
3	37.8481869147084	39.0	39.0	39.0	35.0	39.0
4	37.83132351754181	39.0	39.0	39.0	35.0	39.0
5	37.87542411952751	39.0	39.0	39.0	35.0	39.0
6	37.91373057098257	39.0	39.0	39.0	35.0	39.0
7	37.90599853897531	39.0	38.0	39.0	35.0	39.0
8	38.90069846875813	40.0	39.0	40.0	37.0	40.0
9	38.881586383769736	40.0	40.0	40.0	36.0	40.0
10	38.71985098205802	40.0	39.0	40.0	36.0	40.0
11	38.65192445104123	40.0	39.0	40.0	36.0	40.0
12	38.795766410477164	40.0	39.0	40.0	36.0	40.0
13	38.81177700826893	40.0	40.0	40.0	36.0	40.0
14	38.88772116866004	40.0	40.0	40.0	37.0	40.0
15	38.91379797759799	40.0	40.0	40.0	37.0	40.0
16	38.89767417427092	40.0	40.0	40.0	37.0	40.0
17	38.89059859344862	40.0	40.0	40.0	37.0	40.0
18	38.81918007094057	40.0	40.0	40.0	37.0	40.0
19	38.683434890807156	40.0	39.0	40.0	36.0	40.0
20	38.70895584960637	40.0	39.0	40.0	36.0	40.0
21	38.592793896170484	40.0	39.0	40.0	36.0	40.0
22	38.64892372146835	40.0	39.0	40.0	36.0	40.0
23	38.30741284093675	40.0	38.0	40.0	35.0	40.0
24	38.48316623512602	40.0	39.0	40.0	36.0	40.0
25	38.577560549107915	40.0	39.0	40.0	36.0	40.0
26	38.54754691199021	40.0	39.0	40.0	36.0	40.0
27	38.21014180676504	40.0	39.0	40.0	35.0	40.0
28	38.328390390306225	40.0	39.0	40.0	35.0	40.0
29	38.19215872386829	40.0	39.0	40.0	35.0	40.0
30	38.20721881792283	40.0	38.0	40.0	35.0	40.0
31	38.10859738106901	40.0	38.0	40.0	34.0	40.0
32	38.08157108614761	40.0	38.0	40.0	34.0	40.0
33	38.03648999583269	40.0	38.0	40.0	34.0	40.0
34	38.03994581792056	40.0	38.0	40.0	34.0	40.0
35	37.99168307272704	40.0	38.0	40.0	34.0	40.0
36	37.88302548760779	40.0	38.0	40.0	34.0	40.0
37	37.96203199081955	40.0	38.0	40.0	34.0	40.0
38	37.927712550634226	40.0	38.0	40.0	34.0	40.0
39	37.881214747064305	40.0	38.0	40.0	34.0	40.0
40	37.80005843472444	40.0	38.0	40.0	34.0	40.0
41	37.66553557687497	40.0	38.0	40.0	33.0	40.0
42	37.5782501947237	40.0	37.0	40.0	33.0	40.0
43	37.54512598891416	40.0	37.0	40.0	33.0	40.0
44	37.526827558924616	40.0	37.0	40.0	33.0	40.0
45	37.43248272343394	40.0	37.0	40.0	33.0	40.0
46	37.30196879770476	40.0	37.0	40.0	33.0	40.0
47	37.16700071172304	40.0	36.0	40.0	33.0	40.0
48	37.09501028988211	40.0	36.0	40.0	32.0	40.0
49	36.91949630269626	40.0	36.0	40.0	32.0	40.0
50	36.89284180105779	39.0	36.0	40.0	32.0	40.0
51	36.74108931126018	39.0	35.0	40.0	31.0	40.0
52	36.614191151539075	39.0	35.0	40.0	31.0	40.0
53	36.43487037379043	39.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	13.0
11	26.0
12	77.0
13	249.0
14	532.0
15	965.0
16	1622.0
17	2847.0
18	4280.0
19	6524.0
20	9669.0
21	13629.0
22	18173.0
23	23863.0
24	30516.0
25	38326.0
26	47466.0
27	59322.0
28	72873.0
29	89337.0
30	110351.0
31	137340.0
32	173100.0
33	221657.0
34	299887.0
35	434681.0
36	674157.0
37	1184644.0
38	2178239.0
39	6938861.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.623988910554868	38.62720047173671	18.220540510240678	18.528270107467755
2	25.946818294233715	34.19189997954315	22.58502099743471	17.276260728788426
3	31.064483548284233	24.405038758020975	22.452055381149965	22.078422312544827
4	22.25686586482805	26.26903131056858	22.151958937236454	29.322143887366913
5	21.971268497772726	34.44016926967633	21.942755734318354	21.64580649823259
6	30.195157417933622	23.845391614820592	25.238939228121442	20.72051173912434
7	21.0548438542586	24.536203733011245	32.37593757630707	22.03301483642309
8	29.597258390538272	24.14466602683879	24.630729572096385	21.627346010526548
9	29.975620099760224	24.55881352456979	24.500488404378938	20.965077971291045
10	20.717278413669465	25.31872329521741	33.55858312077285	20.405415170340273
11	21.14038997349691	33.84281043466933	24.676223165845247	20.340576425988516
12	20.714107719216138	24.473643191340763	33.63290263298382	21.17934645645928
13	21.071096599160104	24.92803893644104	24.24983130731177	29.751033157087086
14	20.894735527678325	33.924512576187674	24.381105886554742	20.799646009579256
15	21.05155572667737	33.2422730763338	24.291293030336032	21.414878166652795
16	21.482175177815286	24.371679920821887	24.074127861346238	30.07201704001659
17	30.193442894266266	24.35580296192967	24.24509483781976	21.205659305984305
18	21.183206091929627	24.536446428142238	24.189752519077597	30.090594960850538
19	21.910524255147113	33.40783030004869	23.87127387620998	20.810371568594217
20	30.366077421155985	24.57560646185964	24.198732238924432	20.859583878059944
21	21.75079954345131	33.09575567708927	24.185955514608796	20.967489264850613
22	30.34407045298733	24.27313786876253	24.398000599222108	20.984791079028035
23	21.237781180902836	24.613239864914323	33.109863310187784	21.039115643995054
24	21.14833628181821	33.1185063884013	24.125430480488603	21.607726849291883
25	21.35230979610712	24.441599605174165	24.155211521724308	30.050879076994402
26	21.462015824192274	29.835827704306826	27.805690762404833	20.896465709096066
27	21.785661524687537	23.996802061060997	33.00785306641775	21.209683347833717
28	27.548426094674433	24.19095816585738	27.312448138594892	20.948167600873298
29	27.659705726673455	24.12168044927096	27.34260496583988	20.8760088582157
30	27.14505895808475	24.05938608935706	27.537810139912178	21.257744812646013
31	25.167579030733577	25.099898404686616	27.78178137756418	21.950741187015623
32	23.546673052941124	26.035143664165684	28.436729418493854	21.98145386439934
33	23.241855797285996	25.47096360222832	28.69878535784262	22.58839524264307
34	23.05822952962474	25.46390195680387	28.890647602207338	22.58722091136406
35	22.835615463500336	25.56043198793852	28.990575365175925	22.613377183385218
36	23.08409613326374	25.47251371951661	28.845154008458472	22.598236138761177
37	23.224162539348907	25.5097869943124	28.79363218081069	22.472418285528004
38	23.14083199179033	25.532592507750785	28.92041298569265	22.406162514766237
39	23.16655767567585	25.36896901620867	28.87464538131202	22.589827926803462
40	23.158650511730514	25.666020027671944	28.541620688335062	22.633708772262484
41	23.11290639397546	25.963094525760795	28.581164336936936	22.342834743326804
42	23.279708408846098	25.66058678828772	28.62657181305867	22.433132989807508
43	23.202100769053896	25.62574829367708	28.726812731035	22.445338206234027
44	23.047496141734584	25.522281879121074	28.790007411596147	22.6402145675482
45	23.17187348193217	25.240505003160123	28.69040063251048	22.89722088239722
46	23.3815151018611	25.398914463823434	28.418425508291676	22.801144926023785
47	23.17146638042211	25.46563213822161	28.573030135610995	22.789871345745286
48	23.24260736930456	25.317588108314364	28.627072861071053	22.81273166131002
49	23.133692057613946	25.85555709610422	28.306801405784142	22.70394944049769
50	23.10335516623951	25.738938171223293	28.512747796621795	22.644958865915402
51	23.169955407509786	25.499859980567162	28.409524077196778	22.920660534726267
52	23.291741390018355	25.690774931033484	28.113091546873786	22.904392132074378
53	23.33470625707975	25.84817446679684	27.902776643678216	22.91434263244519
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3401.0
1	2434.5
2	1468.0
3	1255.5
4	1043.0
5	1099.0
6	1155.0
7	1299.0
8	1714.5
9	1986.0
10	2657.5
11	3329.0
12	4826.5
13	6324.0
14	8017.0
15	9710.0
16	13764.5
17	17819.0
18	24625.5
19	31432.0
20	42915.0
21	54398.0
22	226685.5
23	398973.0
24	285288.5
25	176092.0
26	180580.0
27	195869.0
28	211158.0
29	232817.0
30	254476.0
31	274983.5
32	295491.0
33	324540.5
34	353590.0
35	394011.5
36	434433.0
37	474711.0
38	514989.0
39	553788.0
40	592587.0
41	634508.5
42	716186.0
43	755942.0
44	794397.0
45	832852.0
46	861756.5
47	890661.0
48	911698.5
49	932736.0
50	922818.0
51	912900.0
52	886549.5
53	860199.0
54	828833.5
55	797468.0
56	752686.0
57	707904.0
58	635152.0
59	493882.5
60	425365.0
61	370527.0
62	315689.0
63	271445.0
64	227201.0
65	187363.5
66	147526.0
67	118184.0
68	88842.0
69	68782.5
70	48723.0
71	36331.0
72	23939.0
73	17922.0
74	11905.0
75	8982.5
76	4545.5
77	3031.0
78	2368.5
79	1706.0
80	1428.0
81	1150.0
82	920.5
83	691.0
84	474.5
85	258.0
86	178.0
87	98.0
88	80.0
89	62.0
90	57.5
91	53.0
92	41.5
93	17.5
94	5.0
95	6.0
96	7.0
97	5.0
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.2773227E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.97419136857842
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.25685654192601	34.18490072065698
2	15.242109704951815	14.624557756924494
3	5.105963462716357	7.348634048439713
4	2.338975084610475	4.488417532617593
5	1.315952792444108	3.156588554836439
6	0.8718554137232435	2.5095935078214
7	0.626903211016701	2.1052622230424056
8	0.47996281862618845	1.8420662488460047
9	0.38926975408175113	1.680741150868565
>10	2.248401509796577	18.80462861496637
>50	0.09197884738723926	2.947961630185465
>100	0.02958134408548183	2.6039918226668632
>500	0.0015991283243683462	0.510400324197969
>1k	4.4279421212224034E-4	0.3993401140493142
>5k	3.2798243903853095E-5	0.10500546903713968
>10k+	1.1479385366348583E-4	2.687910280843255
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	268535	2.102327000060361	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	18785	0.1470654205080674	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.480212635381803E-5	0.0	0.0	0.0	1.5657750386805152E-5
2	8.611762712742833E-5	0.0	0.0	0.0	2.3486625580207728E-5
3	8.611762712742833E-5	0.0	0.0	0.0	2.3486625580207728E-5
4	1.0177537751423348E-4	0.0	0.0	0.0	3.1315500773610304E-5
5	1.0177537751423348E-4	0.0	0.0	0.0	3.9144375967012876E-5
6	1.0960425270763606E-4	0.0	0.0	0.0	3.9144375967012876E-5
7	1.1743312790103864E-4	0.0	0.0	0.0	3.9144375967012876E-5
8	1.2526200309444122E-4	0.0	0.0	0.0	3.9144375967012876E-5
9	1.2526200309444122E-4	0.0	0.0	0.0	4.6973251160415456E-5
10	1.2526200309444122E-4	0.0	0.0	0.0	4.6973251160415456E-5
11	1.2526200309444122E-4	0.0	0.0	0.0	4.6973251160415456E-5
12	1.4091975348124635E-4	0.0	0.0	0.0	4.6973251160415456E-5
13	1.644063790614541E-4	0.0	0.0	0.0	5.480212635381803E-5
14	1.7223525425485666E-4	0.0	0.0	0.0	5.480212635381803E-5
15	1.8006412944825923E-4	0.0	0.0	0.0	6.263100154722061E-5
16	2.1137963022186955E-4	0.0	0.0	0.0	7.828875193402575E-5
17	2.1137963022186955E-4	7.828875193402576E-6	0.0	0.0	8.611762712742833E-5
18	2.1137963022186955E-4	7.828875193402576E-6	0.0	0.0	9.394650232083091E-5
19	2.1137963022186955E-4	7.828875193402576E-6	0.0	0.0	9.394650232083091E-5
20	2.2703738060867468E-4	7.828875193402576E-6	0.0	0.0	9.394650232083091E-5
21	2.5835288138228497E-4	7.828875193402576E-6	0.0	0.0	9.394650232083091E-5
22	2.5835288138228497E-4	1.5657750386805152E-5	0.0	0.0	1.0960425270763606E-4
23	2.896683821558953E-4	2.3486625580207728E-5	0.0	0.0	1.0960425270763606E-4
24	3.0532613254270047E-4	2.3486625580207728E-5	0.0	0.0	1.0960425270763606E-4
25	3.209838829295056E-4	2.3486625580207728E-5	0.0	7.828875193402576E-6	1.0960425270763606E-4
26	3.3664163331631073E-4	3.1315500773610304E-5	0.0	7.828875193402576E-6	1.0960425270763606E-4
27	3.6012825889651846E-4	3.1315500773610304E-5	7.828875193402576E-6	7.828875193402576E-6	1.0960425270763606E-4
28	3.6795713408992105E-4	3.1315500773610304E-5	7.828875193402576E-6	7.828875193402576E-6	1.0960425270763606E-4
29	3.914437596701288E-4	3.1315500773610304E-5	7.828875193402576E-6	7.828875193402576E-6	1.0960425270763606E-4
30	3.914437596701288E-4	3.1315500773610304E-5	7.828875193402576E-6	7.828875193402576E-6	1.0960425270763606E-4
31	4.149303852503365E-4	3.9144375967012876E-5	7.828875193402576E-6	7.828875193402576E-6	1.0960425270763606E-4
32	4.384170108305442E-4	4.6973251160415456E-5	7.828875193402576E-6	7.828875193402576E-6	1.0960425270763606E-4
33	4.6973251160415454E-4	4.6973251160415456E-5	7.828875193402576E-6	7.828875193402576E-6	1.0960425270763606E-4
34	4.7756138679755714E-4	4.6973251160415456E-5	7.828875193402576E-6	7.828875193402576E-6	1.0960425270763606E-4
35	4.932191371843622E-4	4.6973251160415456E-5	7.828875193402576E-6	1.5657750386805152E-5	1.2526200309444122E-4
36	5.167057627645699E-4	4.6973251160415456E-5	1.5657750386805152E-5	1.5657750386805152E-5	1.4091975348124635E-4
37	5.558501387315828E-4	4.6973251160415456E-5	1.5657750386805152E-5	1.5657750386805152E-5	1.4091975348124635E-4
38	5.558501387315828E-4	4.6973251160415456E-5	1.5657750386805152E-5	1.5657750386805152E-5	1.4091975348124635E-4
39	6.106522650854009E-4	4.6973251160415456E-5	1.5657750386805152E-5	2.3486625580207728E-5	1.4091975348124635E-4
40	6.341388906656087E-4	4.6973251160415456E-5	1.5657750386805152E-5	2.3486625580207728E-5	1.4091975348124635E-4
41	6.576255162458164E-4	4.6973251160415456E-5	1.5657750386805152E-5	2.3486625580207728E-5	1.4091975348124635E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	120340	0.0	44.97495	1
AGCAGTG	129140	0.0	42.023037	2
GTGGTAT	129695	0.0	41.63846	6
AGTGGTA	129670	0.0	41.606617	5
TCAACGC	129975	0.0	41.534298	12
GGTATCA	129530	0.0	41.466534	8
CAACGCA	129630	0.0	41.423668	13
GTATCAA	129630	0.0	41.407352	9
TGGTATC	129830	0.0	41.348995	7
AACGCAG	130845	0.0	41.31201	14
ACGCAGA	130370	0.0	41.302097	15
ATCAACG	130410	0.0	41.235374	11
TATCAAC	130440	0.0	41.144817	10
GCAGTGG	131875	0.0	41.12834	3
CAGTGGT	131570	0.0	41.104015	4
CGCAGAG	131555	0.0	40.924706	16
AGAGTAC	129940	0.0	40.807606	19
GAGTACT	48145	0.0	40.449577	20
CAGAGTA	131360	0.0	40.42372	18
GCAGAGT	133160	0.0	40.10495	17
>>END_MODULE