FastQCFastQC Report
Thu 26 May 2016
SRR805175_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805175_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19954016
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10616025.320242301098686No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC1012480.5074066293221374No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG594040.29770448214534856No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG460930.23099610624748423No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA444360.2226920134773872No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG406380.20365825105081603No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC304770.15273617100437326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT5398450.046.2142641
AGCAGTG5471450.045.6049772
GTGGTAT5525700.045.2401666
AGTGGTA5530850.045.211645
GTATCAA5530900.045.1487739
TGGTATC5538200.045.1329737
GGTATCA5534100.045.1239438
TCAACGC5496700.045.11117612
CAACGCA5500500.045.07531413
GCAGTGG5549850.045.073793
TATCAAC5502350.045.07040810
ACGCAGA5515700.045.0461115
ATCAACG5508150.045.0442811
AACGCAG5508950.045.01640714
AGAGTAC5500200.044.92652519
CGCAGAG5532300.044.91094216
GAGTACT1671550.044.85884520
CAGAGTA5527400.044.84999518
CAGTGGT5573600.044.8269164
GTACTTT1660400.044.75105322