FastQCFastQC Report
Thu 26 May 2016
SRR805174_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805174_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18905937
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10046405.313886320471712No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC826250.4370320286161961No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG578090.30577167373402336No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG446090.23595233603073998No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA422800.2236334544011228No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG383870.2030420391224196No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC291100.1539727970108014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT4928050.046.0968251
AGCAGTG5003050.045.4222372
AGTGGTA5040550.045.1906055
GTGGTAT5041150.045.1740386
GGTATCA5040700.045.121668
GTATCAA5041100.045.1194769
TGGTATC5048000.045.1113437
TCAACGC5005400.045.0896312
CAACGCA5007350.045.071613
ACGCAGA5019400.045.0710815
TATCAAC5011750.045.04422810
AACGCAG5014550.045.02844614
ATCAACG5020550.044.98212411
GCAGTGG5066900.044.969973
AGAGTAC5006000.044.95184319
CGCAGAG5033950.044.93194216
CAGTGGT5069050.044.9073224
CAGAGTA5035600.044.83695618
GAGTACT1565600.044.74396520
GCAGAGT5084900.044.6328517